Genomic features of a high-risk mcr-1.1-positive Escherichia coli ST10 isolated from cattle farm environment

Ali, Arslan; Fontana, Herrison; Sano, Elder; Li, Ruichao; Humayon, Muhammad; Rahman, Sadeequr; Lincopán, Nilton; Mohsin, Mashkoor

doi:10.1007/s11356-021-15437-6

Cited by 10 publications

(8 citation statements)

References 28 publications

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“…In Pakistan, 200 samples from poultry chicken were obtained and analyzed for ESBL production. Results showed that 87% bacterial isolates were E. coli having blaOXA-48, blaNDM, blaCTXM and blaSHV2 as the most reported genes ( Ali et al, 2021 ) making it in line with our results.…”

Section: Discussionsupporting

confidence: 90%

Extended spectrum beta lactamases-producing Escherichia coli in retail chicken meat from Khyber Pakhtunkhwa, Pakistan

Zainab

Ibrar

Sadiq

et al. 2022

Saudi Journal of Biological Sciences

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Section: Discussionsupporting

confidence: 90%

Extended spectrum beta lactamases-producing Escherichia coli in retail chicken meat from Khyber Pakhtunkhwa, Pakistan

Zainab

Ibrar

Sadiq

et al. 2022

Saudi Journal of Biological Sciences

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“…The high prevalence of ST10 in our study was similar to that in a previous report ( Dadashi et al, 2022 ), however, it was different from a report that ST131 was the most common ST for extraintestinal pathogenic E. coli (ExPEC) strains ( Manges et al, 2019 ). To date, E. coli ST10 has been shown to be a high-risk mcr -1-positive isolate from cattle farm environments ( Ali et al, 2021 ); its clonal spread with ∼50% fosfomycin resistance has been found among diarrheal calves in Xinjiang province, China ( He et al, 2021 ). It also has been isolated from poultry in Poland ( Ćwiek et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

et al. 2023

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ObjectiveThe worldwide dissemination of colistin-resistant Escherichia coli (E. coli) endangers public health. This study aimed to better understand the global genomic epidemiology of E. coli isolates carrying mobilized colistin resistance (mcr) genes, providing information to assist in infection and prevention.MethodsEscherichia coli genomes were downloaded from NCBI, and mcr was detected using BLASTP. Per software was used to extract information on hosts, resources, collection data, and countries of origin from GenBank. Sequence types (STs), prevalence of plasmids, antimicrobial resistance genes (ARG), and virulence factors (VF) in these genomes were analyzed. Statistical analyses were performed to assess the relationships between mcr, ARGs, plasmids, and STs.ResultsIn total, 778 mcr-positive isolates were identified. Four mcr variants were detected, with mcr-1 (86.1%) being the most widespread, followed by mcr-9 (5.7%), mcr-5 (4.4%), and mcr-3 (3.0%). Multiple ARGs were identified, with blaCTX–M (53.3%), fosA (28.8%), qnr (26.1%), blaNDM (19.8%), and aac (6’)-Ib-cr (14.5%) being the most common. Overall, 239 distinct STs were identified, of which ST10 (13.8%) was the most prevalent. A total of 113 different VFs were found, terC (99.9%) and gad (83.0%) were most frequently detected. Twenty types of plasmids were identified; IncFIB (64.1%), IncX (42.3%), and IncX (42.3%) were the most common replicons. IncI2 and IncX4 were frequently detected in mcr-1-positive isolates, whereas IncFII, IncI1-I, and IncHI2 were dominant plasmids in mcr-3, mcr-5, and mcr-9-positive isolates, respectively. A higher frequency of ARGs and VFs was observed among ST156 and ST131 isolates.ConclusionOur data indicated that more than half of the mcr-positive E. coli strains carried endemic ARGs and VFs. ST10 and ST156 isolates deserved further attention, given the rapid transmission of ST10 and the convergence of ARGs and VFs in ST156.

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“…Interestingly, all these resistant isolates were harbouring plasmid-mediated gene, mcr-1 indicating its ability of fast dissemination and horizontal transfer. Previous reports from Pakistan have already detected the mcr-1 gene from clinical, cattle, wild birds, poultry and the environment (Ali et al, 2021;Bilal et al, 2021;Mohsin et al, 2017Mohsin et al, , 2019. On top of this, the co-occurrence of ESBL and colistin is limiting the option for treatment in near future presenting a huge challenge for healthcare community.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, prevailed practices indicate that a major share of antibiotics usage is aimed for disease prevention and as growth promoters in Pakistan. On top of this, the availability of antibiotics over the counter encourages its overuse and accumulating pool of MGEs in the environment (Ali et al, 2021;Mohsin et al, 2017Mohsin et al, , 2019Wang et al, 2020) that contribute to the emergence and dissemination of MDRs.…”

Section: Introductionmentioning

confidence: 99%

Incidence and molecular characterization of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan

Shafiq

Rahman

Bilal

et al. 2022

Journal of Applied Microbiology

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Objectives: To investigate the occurrence and molecular features of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan. Methods: A total of 153 E. coli isolates were recovered from 250 faecal samples collected from livestock and poultry. The antibiotic susceptibility, resistant determinants and mobile genetic elements were determined for all the isolates. The clonal relatedness was analysed by MLST. Plasmids harbouring, localization and transferability of mcr-1 gene were carried out by Southern hybridization, S1-PFGE and transconjugation.Results: Out of 153 E. coli strains, 49.01% isolates were ESBLs producers, whereas 18.95% were resistant to colistin and 84.31% of the isolates. Multidrug resistance was found in 84% of the isolates. The ESBL-producing E. coli in buffaloes, cattle, sheep, goat and broilers faecal samples were 60%, 74%, 54%, 50% and 68%, respectively. Among the ESBLs genes, bla CTX-M was the most prevalent group detected in 98.66%, while only mcr-1 of the colistin-resistant genes could be PCR amplified in 29 isolates. The common MGEs found were ISECP1 (35.13%), ISCR1 (33.78%), ISApl1 (20.27%) and Inti1 (58.10%). The most predominant Inc. types found were IncFIB 46.66%, followed by IncFIA 30.66%, IncFIC 26.66%, IncFrepB 26.66%, IncHI2 26.66%, IncP 22.66% and IncX4 21.33%. The most frequent sequence type detected was ST58.Southern blot and S1-PFGE confirmed the plasmid harbouring of mcr-1 gene. Conclusion:The co-occurrence of mcr-1 and ESBLs-encoding genes, along with MGEs in E. coli from healthy food animals in Pakistan, is a major concern.

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Genomic features of a high-risk mcr-1.1-positive Escherichia coli ST10 isolated from cattle farm environment

Cited by 10 publications

References 28 publications

Extended spectrum beta lactamases-producing Escherichia coli in retail chicken meat from Khyber Pakhtunkhwa, Pakistan

Extended spectrum beta lactamases-producing Escherichia coli in retail chicken meat from Khyber Pakhtunkhwa, Pakistan

Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates

Incidence and molecular characterization of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan

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