Genomic DNA methylation signatures in different tissues after ambient air particulate matter exposure

Li, Zhigang; Li, Nannan; Guo, Chen; Li, Xiaoqian; Qian, Yan; Wu, Jing; Yang, Yi‐Ning; Wei, Yongjie

doi:10.1016/j.ecoenv.2019.04.049

Cited by 20 publications

(5 citation statements)

References 41 publications

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“…2.5 exposure(Real et al, 2021). At present, most studies have found that, at the transcriptional level, particulate matter exposure induces DNA hypomethylation, and our research also con rmed this(Li et al, 2019a). m 6 A RNA methylation is a newly discovered and important modi cation method in recent years, which regulates gene expression at the post-transcriptional level(Zhao et al, 2017).…”

supporting

confidence: 76%

TGF-β regulates m 6 A RNA methylation after PM 2.5 exposure

Xue

Zhang

et al. 2022

Preprint

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Atmospheric particulate matter exposure has adverse effects on human health, but its molecular mechanism is complex and need to be explored in depth over time. m6A RNA methylation is an important epigenetic modification that regulates gene expression at the post-transcriptional level. Our previous animal exposure studies found that PM2.5 exposure up-regulated m6A RNA methylation in lung, but the regulatory pathway is currently unclear. PM2.5 can activate transforming growth factor-β (TGF-β), and Smad2/3, a downstream factor of TGF-β, can affect m6A RNA methylation by binding to the RNA methyltransferase complex. Based on the above evidences, the current study aimed to investigate the role of TGF-β signal pathway in PM2.5-induced m6A RNA methylation through animal and A549 cell exposure model. Our results showed that PM2.5 could induce upregulation of m6A RNA methylation, accompanied by increased expression of TGF-β, Smad3, methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14) in both lungs of mice and A549 cell line. Furthermore, the TGF-β inhibition cellular experiments determined that PM2.5 exposure altered the level of m6A RNA methylation, expression of TGF-β, Smad3. Accordingly, it is clear that TGF-β plays an indispensable role in m6A RNA methylation after PM2.5 exposure. Our study demonstrates that PM2.5 exposure influence RNA m6A methylation through the TGF-β signal pathway.

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supporting

confidence: 76%

TGF-β regulates m 6 A RNA methylation after PM 2.5 exposure

Xue

Zhang

et al. 2022

Preprint

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“…The major DNA modification was the methylation of cytosine (5 mC) ( Li Z. et al, 2019 ). The DNA methylation process is mediated by DNMTs ( Wu et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Nrf2−/− regulated lung DNA demethylation and CYP2E1 DNA methylation under PM2.5 exposure

Jiang

Ding

et al. 2023

Front. Genet.

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Cytochrome P450 (CYP450) can mediate fine particulate matter (PM2.5) exposure leading to lung injury. Nuclear factor E2-related factor 2 (Nrf2) can regulate CYP450 expression; however, the mechanism by which Nrf2−/− (KO) regulates CYP450 expression via methylation of its promoter after PM2.5 exposure remains unclear. Here, Nrf2−/− (KO) mice and wild-type (WT) were placed in a PM2.5 exposure chamber (PM) or a filtered air chamber (FA) for 12 weeks using the real-ambient exposure system. The CYP2E1 expression trends were opposite between the WT and KO mice following PM2.5 exposure. After exposure to PM2.5,CYP2E1 mRNA and protein levels were increased in WT mice but decreased in KO mice, and CYP1A1 expression was increased after exposure to PM2.5 in both WT and KO mice. CYP2S1 expression decreased after exposure to PM2.5 in both the WT and KO groups. We studied the effect of PM2.5 exposure on CYP450 promoter methylation and global methylation levels in WT and KO mice. In WT and KO mice in the PM2.5 exposure chamber, among the methylation sites examined in the CYP2E1 promoter, the CpG2 methylation level showed an opposite trend with CYP2E1 mRNA expression. The same relationship was evident between CpG3 unit methylation in the CYP1A1 promoter and CYP1A1 mRNA expression, and between CpG1 unit methylation in the CYP2S1 promoter and CYP2S1 mRNA expression. This data suggests that methylation of these CpG units regulates the expression of the corresponding gene. After exposure to PM2.5, the expression of the DNA methylation markers ten-eleven translocation 3 (TET3) and 5-hydroxymethylcytosine (5hmC) was decreased in the WT group but significantly increased in the KO group. In summary, the changes in CYP2E1, CYP1A1, and CYP2S1 expression in the PM2.5 exposure chamber of WT and Nrf2−/− mice might be related to the specific methylation patterns in their promoter CpG units. After exposure to PM2.5, Nrf2 might regulate CYP2E1 expression by affecting CpG2 unit methylation and induce DNA demethylation via TET3 expression. Our study revealed the underlying mechanism for Nrf2 to regulate epigenetics after lung exposure to PM2.5.

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“…New study designs include meeting-in-the-middle (MITM) (94) and environment-wide association studies. As an example, exposure to PM 2.5 is associated with epigenetic changes in DNA methylation (95,96), and exposure to PFAS is associated with plasma metabolites related to kidney injury (97). Alone, these findings are suggestive of associations between exposures and outcomes.…”

Section: Mind the Gapmentioning

confidence: 99%

Environmental Exposures and Kidney Disease

et al. 2022

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Accumulating evidence underscores the large role played by the environment in the health of communities and individuals. We review the currently known contribution of environmental exposures and pollutants on kidney disease and its associated morbidity. We review air pollutants, such as particulate matter; water pollutants such as trace elements, per- and polyfluorooakyl substances, and pesticides; and extreme weather events and natural disasters. We also discuss gaps in the evidence which at present relies heavily on observational studies and animal models, and propose using recently developed quantitative methods to help bridge the gaps. With the expected increase in the intensity and frequency of many environmental exposures in the decades to come, an improved understanding of their potential effect on kidney disease is crucial to mitigate potential morbidity and mortality.

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Genomic DNA methylation signatures in different tissues after ambient air particulate matter exposure

Cited by 20 publications

References 41 publications

TGF-β regulates m 6 A RNA methylation after PM 2.5 exposure

TGF-β regulates m 6 A RNA methylation after PM 2.5 exposure

Nrf2−/− regulated lung DNA demethylation and CYP2E1 DNA methylation under PM2.5 exposure

Environmental Exposures and Kidney Disease

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