Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene

Nishizawa, Tsutomu; Furuhashi, Makoto; Nagai, Tadashi; Nakai, Toshiko; Muroga, Kiyokuni

doi:10.1128/aem.63.4.1633-1636.1997

Cited by 390 publications

(251 citation statements)

References 8 publications

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“…Therefore, these results clearly suggest that human cell lines can support the binding of the betanodavirus, possibly by sialic acid, as is the case with fish E-11 cells. Desler et al reported that viral coat protein expression was detected in HeLa cells when these cells were infected with Dicentrarchus labrax encephalitis virus (DlEV) [7], a member of the betanodavirus genus belonging to the RGNNV genotype [15,30]. However, the data presented by Desler et al did not confirm that the human cell line HeLa is susceptible to betanodavirus infection, as their results showed only a limited number of cells which were diffusely stained with antibody against the betanodavirus.…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that the RNA2-encoded coat protein controls host specificity. Indeed, a variable region of RNA2, encoding the C-terminal region of the coat protein, is used to divide betanodaviruses into four genotypes [25,29,30]. In addition, the C-terminal sequences of the coat protein are displayed on the surface of the virion [38], suggesting that this region interacts with the cell surface.…”

Section: Discussionmentioning

confidence: 99%

“…The betanodaviruses are classified into four genotypes based on the phylogenetic analysis of their genomic RNA2 (coat protein) sequences. The genotypes are: striped jack nervous necrosis virus (SJNNV), barfin flounder nervous necrosis virus (BFNNV), tiger puffer nervous necrosis virus (TPNNV) and redspotted grouper nervous necrosis virus (RGNNV) [25,29,30].…”

Section: Introductionmentioning

confidence: 99%

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Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

et al. 2007

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The determination of the host ranges of viruses is important because of the possible emergence of infectious agents, which may result from the zoonotic transmission of animal viruses to humans. The family Nodaviridae, whose members are non-enveloped, positive-stranded bipartite RNA viruses, is comprised of the genera Alphanodavirus and Betanodavirus, whose members predominantly infect insects and fish, respectively. The alphanodaviruses can also infect suckling mice and suckling hamsters, resulting in paralysis and death. Pigs near the site of isolation of the Nodamura virus (NoV), an alphanodavirus, have been reported to have high levels of NoV neutralizing antibody, suggesting that they may be part of the natural host range of this virus. Betanodaviruses are the causative agents of viral nervous necrosis, which occurs in several species of fish. However, little is known regarding the mechanism of infection of these viruses. Whether betanodaviruses can infect hosts other than fish remains unclear. In this study, we examined the possibility that a betanodavirus, redspotted grouper nervous necrosis virus (RGNNV), can infect human cell lines and showed that this virus can attach to the cells but cannot penetrate them, although human cells can support the replication of the betanodavirus when viral RNAs are transfected. The betanodavirus in its present form cannot infect human cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

et al. 2007

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“…Betanodaviruses have been classified into four genotypes, designated SJNNV (striped jack nervous necrosis virus), TPNNV (tiger puffer nervous necrosis virus), RGNNV (red grouper nervous necrosis virus) and BFNNV (barfin flounder nervous necrosis virus), using a partial sequence of RNA2, the T4 region, which is a highly variable region of around 400 nt [77,78]. These types exhibit different capabilities for infecting fish species.…”

Section: Betanodaviruses -The Role Of Mutation and Reassortment In Homentioning

confidence: 99%

Host range, host specificity and hypothesized host shift events among viruses of lower vertebrates

Bandı́n

Dopazo

2011

Vet Res

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The successful replication of a viral agent in a host is a complex process that often leads to a species specificity of the virus and can make interspecies transmission difficult. Despite this difficulty, natural host switch seems to have been frequent among viruses of lower vertebrates, especially fish viruses, since there are several viruses known to be able to infect a wide range of species. In the present review we will focus on well documented reports of broad host range, variations in host specificity, and host shift events hypothesized for viruses within the genera Ranavirus, Novirhabdovirus, Betanodavirus, Isavirus, and some herpesvirus.

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“…Betanodaviruses have been isolated from more than 30 marine fish species from 14 families and are classified into four genotypes based on a phylogenetic analysis of the coat protein sequences. The genotypes are: striped jack nervous necrosis virus (SJNNV), barfin flounder nervous necrosis virus (BFNNV), tiger puffer nervous necrosis virus (TPNNV) and redspotted grouper nervous necrosis virus (RGNNV) [18,20,21]. Effective control of betanodavirus infection is urgently needed to reduce the significant economic loss caused by the virus in the fish industry.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of betanodavirus infection by inhibitors of endosomal acidification

et al. 2007

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Betanodaviruses, members of the family Nodaviridae, have small positive-stranded bipartite RNA genomes and are the causal agent of viral nervous necrosis (VNN) in many species of marine farmed fish. In the aquaculture industry, outbreaks of betanodavirus infection and spread in larval and juvenile fish result in devastating damage and heavy economic loss. Although an urgent need exists to develop drugs that inhibit betanodavirus infection, there have been no reports about anti-betanodavirus drugs. Recently, it was reported that betanodaviruses were detected in the endosomes of infected cells, suggesting that betanodaviruses enter fish cells by endocytosis. This finding prompted us to examine whether blocking this endosomal pathway could provide a target for antiviral drug development. In this study, we examined the inhibitory effect of several lysosomotropic agents against betanodavirus infection in fish E-11 cells. The presence of 1 mM NH4Cl or 1 microM chloroquine in the medium inhibited the entry of betanodaviruses into cells and inhibited viral infection. The lysosomotropic agents bafilomycin A1 and monensin also inhibited virus-induced cytopathology and virus production. Our data demonstrate that inhibitors of endosomal acidification are candidates as antiviral agents against betanodavirus.

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Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene

Cited by 390 publications

References 8 publications

Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

Potential for the replication of the betanodavirus redspotted grouper nervous necrosis virus in human cell lines

Host range, host specificity and hypothesized host shift events among viruses of lower vertebrates

Inhibition of betanodavirus infection by inhibitors of endosomal acidification

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