Genomic characterization of Salmonella Uzaramo for human invasive infection

Xu, Xuebin; Chen, Yan; Pan, Hang; Pang, Zaiyuan; Li, Fang; Peng, Xianqi; Ed-Dra, Abdelaziz; Li, Yan; Yue, Min

doi:10.1099/mgen.0.000401

Cited by 37 publications

(36 citation statements)

References 44 publications

(57 reference statements)

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“…We also noticed that the dominant plasmid was IncFIB(K)-1-Kpn3. Most of the S. enterica isolates in this study that carried the IncFIB(K)-1-Kpn3 plasmids replicon were multidrug-resistant, which is consistent with the findings described previously (47)(48)(49)(50). We also observed that IncFIB(K)-1-Kpn3-positive strains carry more antimicrobial resistance than isolates with other replicon types.…”

Section: Discussionsupporting

confidence: 92%

Characterization of Salmonella Resistome and Plasmidome in Pork Production System in Jiangsu, China

et al. 2020

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The prevalence of antimicrobial resistance in zoonotic Salmonella is a significant ongoing concern over the world. Several reports have investigated the prevalence of Salmonella infections in the farm animals in China; however, there is only limited knowledge about the Salmonella cross-contamination in the slaughterhouses. Moreover, the application of genomic approaches for understanding the cross-contamination in the food-animal slaughterhouses is still in its infancy in China. In the present study, we have isolated 105 Salmonella strains from pig carcasses and environment samples collected from four independent slaughterhouses in Jiangsu, China. All the Salmonella isolates were subjected to whole genome sequencing, bioinformatics analysis for serovar predictions, multi-locus sequence types, antimicrobial resistance genes, and plasmid types by using the in-house Galaxy platform. The antimicrobial resistance of Salmonella isolates was determined using a minimal inhibitory concentration assay with 14 antimicrobials. We found that the predominant serovar and serogroup was S. Derby and O:4(B), with a prevalence of 41.9 and 55%, respectively. All the isolates were multidrug-resistant and the highest resistance was observed against antimicrobials tetracycline (95.4%) and trimethoprim and sulfamethoxazole (90.9%). Additionally, the colistin-resistant determinant mcr-1 gene was detected in five (4.8%) strains. Our study demonstrated the prevalence of antimicrobial resistance in Salmonella strains isolated from pig slaughterhouses in China and suggested that the genomic platform can serve as routine surveillance along with the food-chain investigation.

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Section: Discussionsupporting

confidence: 92%

Characterization of Salmonella Resistome and Plasmidome in Pork Production System in Jiangsu, China

et al. 2020

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“…Javiana isolates harboring cdtB , pltA , and pltB caused cytoplasmic and nuclear enlargement together with cell cycle arrest in G2/M phase, increased invasion and cytotoxicity toward HeLa cells that are characteristic of CDT activity ( Mezal et al, 2014a ). Recently, Xu et al (2020) reported the presence of the cdtB, pltA , and pltB genes in iNTS serovar S . Uzaramo, which showed a higher killing rate than the classic gastrointestinal infectious agent S .…”

Section: Discussionmentioning

confidence: 99%

Global Genomic Characterization of Salmonella enterica Serovar Telelkebir

Qiu

Nambiar

et al. 2021

Front. Microbiol.

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Non-typhoidal Salmonella (NTS) is a common cause for self-limiting gastroenteritis, representing a public health concern globally. NTS is one of the leading causes of foodborne illnesses in China; however, the invasive infection caused by NTS is largely underappreciated. Here, we reported an NTS invasive infection caused by an infrequently reported serovar Telelkebir (13,23:d:e,n,z15) strain FJ001 in China, which carries antimicrobial-resistant genes [fosA7 and aac(6′)-Iaa] and typhoid-toxin genes (cdtB, pltA, and pltB). By conducting the whole genomic sequencing, we also investigated the relatedness of this strain with an additional 120 global contextual Salmonella enterica serovar Telelkebir (S. Telelkebir) isolates, and assessed the antimicrobial-resistant determinants and key virulence factors using the available genomic dataset. Notably, all 121 (100%) of the S. Telelkebir strains possessed the typhoid toxin genes cdtB, pltA, and pltB, and 58.67% (71/121) of S. Telelkebir harbored antimicrobial-resistant gene fosaA7. The study by core genome multilocus sequence typing (cgMLST) and core single-nucleotide polymorphism (SNP)-based phylogenomic analysis demonstrated that the S. Telelkebir isolates from different sources and locations clustered together. This suggests that regular international travels might increase the likelihood of rapid and extensive transmissions of potentially pathogenic bacteria. For the first time, our study revealed the antimicrobial resistance, virulence patterns, and genetic diversity of the serovar S. Telelkebir isolate in humans and similar isolates over the world. The present study also suggests that genomic investigation can facilitate surveillance and could offer added knowledge of a previously unknown threat with the unique combination of virulent and antimicrobial-resistant determinants.

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“…The genomic DNA library was constructed using Nextera XT DNA library construction kit (Illumina, USA, no: FC-131-1024), followed by genomic sequencing using Miseq Reagent Kit v2 300cycle kit (Illumina, USA, No: MS-102-2002). High-throughput genome sequencing was achieved by the Illumina Miseq sequencing platform, as previously described [ 26 , 27 , 28 ]. The quality of sequencing was checked with FastQC toolkit, while low-quality sequences and joint sequences were removed with trimmomatic [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Multidrug Resistance Patterns of Emerging Salmonella enterica Serovar Rissen along the Food Chain in China

Biswas

et al. 2020

Antibiotics

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Salmonella spp. are recognized as important foodborne pathogens globally. Salmonella enterica serovar Rissen is one of the important Salmonella serovars linked with swine products in numerous countries and can transmit to humans by food chain contamination. Worldwide emerging S. Rissen is considered as one of the most common pathogens to cause human salmonellosis. The objective of this study was to determine the antimicrobial resistance properties and patterns of Salmonella Rissen isolates obtained from humans, animals, animal-derived food products, and the environment in China. Between 2016 and 2019, a total of 311 S. Rissen isolates from different provinces or province-level cities in China were included here. Bacterial isolates were characterized by serotyping and antimicrobial susceptibility testing. Minimum inhibitory concentration (MIC) values of 14 clinically relevant antimicrobials were obtained by broth microdilution method. S. Rissen isolates from humans were found dominant (67%; 208/311). S. Rissen isolates obtained from human patients were mostly found with diarrhea. Other S. Rissen isolates were acquired from food (22%; 69/311), animals (8%; 25/311), and the environment (3%; 9/311). Most of the isolates were resistant to tetracycline, trimethoprim-sulfamethoxazole, chloramphenicol, streptomycin, sulfisoxazole, and ampicillin. The S. Rissen isolates showed susceptibility against ceftriaxone, ceftiofur, gentamicin, nalidixic acid, ciprofloxacin, and azithromycin. In total, 92% of the S. Rissen isolates were multidrug-resistant and ASSuT (27%), ACT (25%), ACSSuT (22%), ACSSuTAmc (11%), and ACSSuTFox (7%) patterns were among the most prevalent antibiotic resistance patterns found in this study. The widespread dissemination of antimicrobial resistance could have emerged from misuse of antimicrobial agents in animal husbandry in China. These findings could be useful for rational antimicrobial usage against Salmonella Rissen infections.

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Genomic characterization of Salmonella Uzaramo for human invasive infection

Abstract: Salmonella is composed of a wide variety of serovars, causing human self-limited gastrointestinal illnesses or invasive infections. Invasive non-typhoida… Show more

Cited by 37 publications

References 44 publications

Characterization of Salmonella Resistome and Plasmidome in Pork Production System in Jiangsu, China

Characterization of Salmonella Resistome and Plasmidome in Pork Production System in Jiangsu, China

Global Genomic Characterization of Salmonella enterica Serovar Telelkebir

Characterization of Multidrug Resistance Patterns of Emerging Salmonella enterica Serovar Rissen along the Food Chain in China

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