Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare blaTEM-61

Hayashi, Wataru; Yoshida, Satoshi; Izumi, Katsutoshi; Koide, Shota; Soga, Eiji; Takizawa, Shino; Arakawa, Yoshichika; Nagano, Yukiko; Nagano, Noriyuki

doi:10.1016/j.jgar.2021.03.010

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…21 In our study, Carbapenems more specifically imipenem had low resistance rates (36.7%) as compared to most of other drugs, which is comparable to the study done by Hayashi et al in June 2021 in which all the isolates included in study were sensitive to Carbapenems. 22 The work done by Xu Q et al in Tertiary hospital in China in year 2020 also supported our study findings by demonstrating Amikacin to be the most sensitive drug. 23 Aminoglycosides were also found to be most sensitive for this bacterium in study done by Ferreira et al 15 An extensive 8-year study done in Taiwan, revealed Ceftazidime and Imipenem with consistently high susceptibility rates to Serratia species while ciprofloxacin had highest resistance rate.…”

Section: Discussionsupporting

confidence: 89%

Antibiotic Sensitivity Pattern of Serratia Species from Clinical Samples at a Tertiary Care Hospital in Rawalpindi

Irfan¹,

Sadiq²,

Amer³

et al. 2022

J. Bahria Uni. Med. Dent. Coll.

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Objective. To determine the antibiotic resistance profile of Serratia spp isolated from various clinical specimens. Study Design and Settings: A descriptive cross-sectional study on antibiotic resistance profile of Serratia spp isolated from various clinical specimens was carried out in the Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, from 1st July 2017 to 30th June 2021. Methodology: 464 clinical specimens yielding growth of Serratia spp were included in the study. Serratia spp were identified by using Standard Microbiological procedures. Modified Kirby Bauer disc diffusion method was used for Antibiotic Susceptibility testing. The clinical data was analyzed prospectively from July 2017 to June 2021 for a period of 4 years. The spectrum of diseases caused by Serratia spp along with resistance profiles were analyzed. . Data obtained was analysed using SPSS 24. Results: High yield of this bug was obtained from pus and tissue specimens 150 (32%).130(28%) isolates were retrieved from blood cultures, whereas respiratory specimens contributed to 89(19 %) isolates of Serratia spp. According to the antimicrobial susceptibility pattern, 154 (33.3%) isolates were sensitive to Meropenem, 150(32.2%) were susceptible to Doxycycline and 118 (25.5%) to Amikacin, making them the preferred antibiotics to be used in our setup. Conclusion: Serratia marcescens isolates are increasingly resistant to antibiotics. Clinical isolates of Serratia exhibited highest resistance to Ciprofloxacin, Ceftriaxone, Gentamicin and Piperacillin/tazobactam

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Section: Discussionsupporting

confidence: 89%

Antibiotic Sensitivity Pattern of Serratia Species from Clinical Samples at a Tertiary Care Hospital in Rawalpindi

Irfan¹,

Sadiq²,

Amer³

et al. 2022

J. Bahria Uni. Med. Dent. Coll.

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“…This gene array ( int I1 -bla VIM-1 -aac(6’)Ib-dfrB1-aadA1-catB2-qacEΔ1/sul1 ) has been detected in a variety of plasmids and species of Enterobacterales collected in our hospital for years 16,19 . The IS 26 - msr(E) - mph(E) -IS 26 transposon conferring resistance to macrolides and triamilides but not lincosamides (and widely spread in the plasmids of Enterobacterales and water systems 32 ) was detected upstream from the class 1 integron ( Fig. 5C ).…”

Section: Resultsmentioning

confidence: 99%

Long-term dynamics of the “Serratia marcescenscomplex” in the hospital-built environment

Aracil-Gisbert,

Fernández-De-Bobadilla,

Guerra-Pinto

et al. 2023

Preprint

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Serratia marcescensis an opportunistic pathogen historically associated with abrupt, unpredictable, and severe outbreaks in hospital intensive care units (ICUs) and, more recently, with the spread of acquired genes encoding carbapenem resistance. However, the population biology and ecology ofS. marcescensin the hospital ecosystem is still poorly understood. Here, we combine epidemiological information of 1417Serratiaisolates collected from the sinks of a large ICU ward that underwent significant demographic and operational changes (2019-2020) and 99 non-redundant outbreak/non-outbreak isolates from the same hospital (2003-2019), with genomic data of 165 isolates. We first report hospital sinks as reservoirs of heterogeneous and coexistent populations of theS. marcescens complex(SMC). A novel SMC clade congruent with the recently describedSerratia neveispecies is predominant, exhibiting chromosomal AmpC β-lactamase with an unusual basal expression in contrast to one of the major features ofS. marcescens. PersistentSerratiasink strains are identical to those involved in clonal and polyclonal outbreaks of VIM-1 and OXA-48 producers since at least 2017. The 'source-sink' dynamics of SMC populations able to acquire the highly conserved plasmids such as IncL carryingblaVIM-1 orblaOXA-48 offer novel insights that might improve interventions to control outbreaks and treatSerratiainfections in high-risk hospital areas.

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“…WGS and de novo assembly of three representative carbapenem-resistant isolates were conducted in a manner like that described previously ( 50 ). Briefly, the genome was sequenced using the 150-bp paired-end method with the NovaSeq6000 platform (Illumina, San Diego, CA), and the resulting raw reads were assembled de novo into scaffolds using the SPAdes v.3.14.1.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Resistance and Type III Secretion System Virulotypes of Pseudomonas aeruginosa Isolates from Dogs and Cats in Primary Veterinary Hospitals in Japan: Identification of the International High-Risk Clone Sequence Type 235

et al. 2021

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Pseudomonas aeruginosa is an environmentally ubiquitous and important opportunistic human pathogen responsible for life-threatening health care-associated infections. Because of its extensive repertoire of virulence determinants and intrinsic and acquired resistance mechanisms, the organism could be one of the most clinically and epidemiologically important causes of morbidity and mortality.

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Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare blaTEM-61

Cited by 6 publications

References 19 publications

Antibiotic Sensitivity Pattern of Serratia Species from Clinical Samples at a Tertiary Care Hospital in Rawalpindi

Antibiotic Sensitivity Pattern of Serratia Species from Clinical Samples at a Tertiary Care Hospital in Rawalpindi

Long-term dynamics of the “Serratia marcescenscomplex” in the hospital-built environment

Antimicrobial Resistance and Type III Secretion System Virulotypes of Pseudomonas aeruginosa Isolates from Dogs and Cats in Primary Veterinary Hospitals in Japan: Identification of the International High-Risk Clone Sequence Type 235

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