Antimicrobial Resistance and Type III Secretion System Virulotypes of Pseudomonas aeruginosa Isolates from Dogs and Cats in Primary Veterinary Hospitals in Japan: Identification of the International High-Risk Clone Sequence Type 235

Hayashi, Wataru; Izumi, Katsutoshi; Yoshida, Satoshi; Takizawa, Shino; Sakaguchi, Kanae; Iyori, Keita; Minoshima, Kenichi; Takano, Shinya; Kitagawa, Maki; Nagano, Yukiko; Nagano, Noriyuki

doi:10.1128/spectrum.00408-21

Cited by 12 publications

(18 citation statements)

References 49 publications

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“…An earlier report from a tertiary hospital in Daejeon, Korea indicates a lack of any resistance genes in ST1455 but on the contrary, all our ST1455 isolates were carbapenem-resistant [41]. Carbapenem resistance has been observed in ST3045 isolates isolated from dogs in Japan and it was true for our isolates except that they were isolated from the human host [42]. P. aeruginosa strain ST663 has been observed in France and also been isolated from healthy human volunteers in Spain where it has an imipenem breakpoint of 4mg/L which groups it into sensitive category.…”

Section: Plasmid Analysiscontrasting

confidence: 47%

Genomic epidemiology and mechanisms of antibiotic resistance in Pseudomonas aeruginosa isolated from children’s hospital in Shenzhen, China

Patil

Chen

Mai

et al. 2022

Preprint

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Background The emergence of multi-drug-resistant Pseudomonas aeruginosa poses a global threat worldwide. We studied the molecular epidemiology and antibiotic resistance mechanisms in 294 clinical isolates of P. aeruginosa from a paediatric hospital. Methods Isolates were identified using an API-20 kit and antimicrobial susceptibility testing was performed using the VITEK®2 compact. β-lactamases, plasmid types and sequence types were determined by PCR and sequencing. The double-disc diffusion method for ESBL was performed. Results 56% (n = 164) isolates were resistant to piperacillin-tazobactam followed by cefepime 40% (n = 117), ceftazidime 39% (n = 115), imipenem 36% (n = 106), meropenem 33% (n = 97), ciprofloxacin 32% (n = 94). 42% (n = 126) isolates were positive for ESBL by double-disc diffusion. The blaCTX-M-15 cephalosporinase was observed in 32% (n = 40/126) while 26% (n = 33/126) were positive for blaNDM-1 carbapenemase. Aminoglycoside resistance gene aac(3)IIIa was observed in 16% (n = 20/126) and glycylcycline resistance gene tetA(Aa) in 12% (n = 15/126) of the isolates. A total of 23 sequence types were detected, ST1963, 12% (n = 16) followed by ST381, 11% (n = 14); ST234, 10% (n = 13; ST145, 58% (n = 10); ST304, 57% (n = 9); ST663 5% (n = 7) and a novel strain. In ESBL-producing P. aeruginosa, 12 different Incompatibility groups (Inc) were observed, the most common being IncFI, IncFIS and IncA/C. The MOBP was the most common plasmid type followed by MOBH, MOBF and MOBQ. Conclusion The spread of antibiotic resistance is likely due to clonal spread and circulation of different plasmids in clinical strains of P. aeruginosa.

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Section: Plasmid Analysiscontrasting

confidence: 47%

Genomic epidemiology and mechanisms of antibiotic resistance in Pseudomonas aeruginosa isolated from children’s hospital in Shenzhen, China

Patil

Chen

Mai

et al. 2022

Preprint

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“…However, some rare cases of carbapenemase expression, especially VIM-2, have been reported in different countries in P. aeruginosa strains isolated from dogs, cattle, and fowl [ 90 , 91 ], but to our knowledge not yet in horses. Some studies even suggest the existence of zoonotic transmission from animals to humans [ 92 ] and from humans to animals [ 93 , 94 ], but in an anecdotal manner, including the case of a transmission of a VIM-2 strain from humans to animals in Brazil [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples

et al. 2022

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Pseudomonas aeruginosa is one of the leading causes of healthcare-associated infections in humans. This bacterium is less represented in veterinary medicine, despite causing difficult-to-treat infections due to its capacity to acquire antimicrobial resistance, produce biofilms, and persist in the environment, along with its limited number of veterinary antibiotic therapies. Here, we explored susceptibility profiles to antibiotics and to didecyldimethylammonium chloride (DDAC), a quaternary ammonium widely used as a disinfectant, in 168 P. aeruginosa strains isolated from animals, mainly Equidae. A genomic study was performed on 41 of these strains to determine their serotype, sequence type (ST), relatedness, and resistome. Overall, 7.7% of animal strains were resistant to carbapenems, 10.1% presented a multidrug-resistant (MDR) profile, and 11.3% showed decreased susceptibility (DS) to DDAC. Genomic analyses revealed that the study population was diverse, and 4.9% were ST235, which is considered the most relevant human high-risk clone worldwide. This study found P. aeruginosa populations with carbapenem resistance, multidrug resistance, and DS to DDAC in equine and canine isolates. These strains, which are not susceptible to antibiotics used in veterinary and human medicine, warrant close the setting up of a clone monitoring, based on that already in place in human medicine, in a one-health approach.

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“…P. aeruginosa expresses six types of secretion systems ( Figure 1 ) that participate in the transport of several virulence factors, including enzymes, exotoxins, and other proteins [ 20 , 44 , 48 , 54 , 55 , 56 , 57 , 58 ].…”

Section: Virulencementioning

confidence: 99%

“…In two-step secretion systems, the protein is temporarily stored in the periplasmic space located between the inner and outer membranes, before being released to the external environment ( Figure 1 ). These differences affect the efficiency and specificity of protein transport [ 20 , 44 , 55 , 56 , 57 , 58 ].…”

Section: Virulencementioning

confidence: 99%

“…P. aeruginosa infections can range from mild to life-threatening conditions, associated with treatment failure [ 1 , 5 ]. This species is also a major threat in veterinary medicine, being associated with chronic otitis externa, pyoderma, conjunctivitis, septicemia, lower urinary tract infections, pneumonia, and bacterial endocarditis [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms of Antibiotic and Biocide Resistance That Contribute to Pseudomonas aeruginosa Persistence in the Hospital Environment

Verdial¹,

Serrano

Tavares

et al. 2023

Biomedicines

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Pseudomonas aeruginosa is an opportunistic bacterial pathogen responsible for multiple hospital- and community-acquired infections, both in human and veterinary medicine. P. aeruginosa persistence in clinical settings is worrisome and is a result of its remarkable flexibility and adaptability. This species exhibits several characteristics that allow it to thrive under different environmental conditions, including the ability to colonize inert materials such as medical equipment and hospital surfaces. P. aeruginosa presents several intrinsic mechanisms of defense that allow it to survive external aggressions, but it is also able to develop strategies and evolve into multiple phenotypes to persevere, which include antimicrobial-tolerant strains, persister cells, and biofilms. Currently, these emergent pathogenic strains are a worldwide problem and a major concern. Biocides are frequently used as a complementary/combination strategy to control the dissemination of P. aeruginosa-resistant strains; however, tolerance to commonly used biocides has also already been reported, representing an impediment to the effective elimination of this important pathogen from clinical settings. This review focuses on the characteristics of P. aeruginosa responsible for its persistence in hospital environments, including those associated with its antibiotic and biocide resistance ability.

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Antimicrobial Resistance and Type III Secretion System Virulotypes of Pseudomonas aeruginosa Isolates from Dogs and Cats in Primary Veterinary Hospitals in Japan: Identification of the International High-Risk Clone Sequence Type 235

Cited by 12 publications

References 49 publications

Genomic epidemiology and mechanisms of antibiotic resistance in Pseudomonas aeruginosa isolated from children’s hospital in Shenzhen, China

Genomic epidemiology and mechanisms of antibiotic resistance in Pseudomonas aeruginosa isolated from children’s hospital in Shenzhen, China

Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples

Mechanisms of Antibiotic and Biocide Resistance That Contribute to Pseudomonas aeruginosa Persistence in the Hospital Environment

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