Genomic changes in transgenic rice (Oryza sativa L.) plants produced by infecting calli with Agrobacterium tumefaciens

Labra, Massimo; Cristian, Savini; Bracale, Marcella; Pelucchi, Nilla; Colombo, Lucia; Bardini, Mauro; Sala, F.

doi:10.1007/s002990100329

Cited by 58 publications

(36 citation statements)

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“…Also, the level of polymorphism in 10 randomly selected transgenic rice (cv Taipei 309) was examined using either RAPD or AFLP analysis. 32 They found that "the value of the genomic similarity was 100% in the case of the control plants and 96-98% in the case of the transgenic population. "…”

Section: Resultsmentioning

confidence: 99%

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Ismail¹

2013

GM Crops & Food

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Section: Resultsmentioning

confidence: 99%

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Ismail¹

2013

GM Crops & Food

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“…There are several examples of attempts to compare different transformation techniques with respect to their impact on the genome, which typically employ the analysis of variability using AFLP (amplified fragment length polymorphism) and RAPD (random amplified polymorphic DNA) techniques. For instance, Labra et al (2001) compared transgenic rice populations obtained with different transformation techniques with non-transgenic rice. In this study, comparison data were also taken from other experiments reported by Arencibia and coworkers (1998) and Bao and coworkers (1996).…”

Section: The Effect Of Integrated Dna On the Host Genome -Insertion Ementioning

confidence: 99%

Unintended consequences of plant transformation: A molecular insight

Filipecki¹,

Malepszy²

2006

J Appl Genet

112

104

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Plant genomes are dynamic structures having both the system to maintain and accurately reproduce the information encoded therein and the ability to accept more or less random changes, which is one of the foundations of evolution. Crop improvement and various uncontrolled stress factors can induce unintended genetic and epigenetic variations. In this review it is attempted to summarize factors causing such changes and the molecular nature of these variations in transgenic plants. Unintended effects in transgenic plants can be divided into three main groups: first, pleiotropic effects of integrated DNA on the host plant genome; second, the influence of the integration site and transgene architecture on transgene expression level and stability; and third, the effect of various stresses related to tissue handling, regeneration and clonal propagation. Many of these factors are recently being redefined due to new researches, which apply modern highly sensitive analytical techniques and sequenced model organisms. The ability to inspect large portions of genomes clearly shows that tissue culture contributes to a vast majority of observed genetic and epigenetic changes. Nevertheless, monitoring of thousands transcripts, proteins and metabolites reveals that unintended variation most often falls in the range of natural differences between landraces or varieties. We expect that an increasing amount of evidence on many important crop species will support these observations in the nearest future.

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“…It has been observed in different transgenic plant populations, such as rice (Bao et al 1993;Arenciba et al 1998;Labra et al 2001), barley (Bregitzer et al 1998), tomato (Soniya et al 2001, potato (Dale and McPartlan 1992;Beaujean et al 1998), poplar (Wang et al 1996), cotton (Sachs et al 1998), tobacco (Touraev et al 1997) and sugarcane (Arencibia et al 1999). The analysis of three successive generations of self pollinated transgenic rice plants has shown that genomic changes are carried to the progeny, where they segregate upon sexual reproduction (Bao et al 1993).…”

Section: Introductionmentioning

confidence: 99%

“…Variation has been correlated with the stress imposed by the presently utilised transformation procedure, depending on plant differentiation from in vitro cell culture. The extent of somaclonal variation, calculated as frequency of DNA changes, has been correlated with the extent and severity of the in vitro stress (Sala et al 2000;Labra et al 2001). …”

Section: Introductionmentioning

confidence: 99%

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

et al. 2004

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The occurrence of DNA modification is an undesired phenomenon accompanying plant cell transformation. The event has been correlated with the stress imposed by the presently utilised transformation procedures, all depending on plant differentiation from in vitro cell culture, but other causes have not been excluded. In this work, transgenic Arabidopsis thaliana plants have been produced by an approach that does not require cell dedifferentiation, being based on in planta Agrobacterium-mediated gene transfer by flower infiltration, which is followed by recovery and selection of transgenic progeny. Genomic DNA changes in transgenic and control plants have been investigated by AFLP and RAMP analysis. Results show no statistically relevant genomic modifications in transgenic plants, as compared with control untreated plants. Variations were observed in callus-derived A. thaliana plants, thus supporting the conclusion that somaclonal variation is essentially correlated with the stress imposed by the in vitro cell culture, rather than with the integration of a foreign gene.

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Genomic changes in transgenic rice (Oryza sativa L.) plants produced by infecting calli with Agrobacterium tumefaciens

Cited by 58 publications

References 0 publications

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Evaluation of genetically modified sugarcane lines carryingCry1AC gene using molecular marker techniques

Unintended consequences of plant transformation: A molecular insight

Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

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