Genomic and proteomic investigation of preeclampsia

Abstract

Abstract. The aim of this study was to use proteomic and transcriptomic approaches to examine differences in protein and gene expression in maternal plasma between normal and preeclamptic pregnancies. Preeclampsia and control groups were compared with respect to the expression of CD34 and CD133 genes by quantitative polymerase chain reaction (qPCR) and heat shock protein (Hsp)27 and 70 by western blotting in blood samples from the pregnant women. Blood samples were obtained at gestational week 12-14 from 65 healthy pregnant women. Fetal DNA was isolated from the maternal blood and CD34 and CD133 were amplified by qPCR. Western blot analysis was used to examine the expression levels of Hsp27 and Hsp70 proteins. The analysis of CD133 by qPCR was unsuccessful in 7 women as the levels of fetal DNA were in the collected maternal blood samples were insufficient. Measurements of CD34 and CD133 were performed in 57 and 50 women, respectively. Preeclampsia developed in 6 (10.5%) of 57 women. qPCR results of 8 healthy pregnant women were used for the calibration of CD34 and CD133 levels, and the results for the remaining women were compared with the calibration values. CD34 expression was decreased in 30 (52.6%) and increased in 27 (47.4%) of 57 women. CD133 expression was decreased in 14 (28%) and increased in 36 (72%) of 50 women. CD34 expression was increased in 2 (33%) and 25 (49%) and decreased in 4 (66%) and 25 (51%) women with and without preeclampsia, respectively (P=0.467). CD133 expression was increased in 4 (66%) and 32 (72%); and decreased in 2 (33%) and 12 (28%) women with and without preeclampsia, respectively (P=0.756). Western blotting showed that the expression of Hsp27 and Hsp70 in the maternal serum of the preeclampsia group was significantly higher than that in the normal pregnancy group. CD34 and CD133 were found to be inadequate for use in the prediction of preeclampsia. However, it is noteworthy that CD133 levels were increased in 66 and 72% of women with and without preeclampsia, respectively. Hsps are expressed under various pathological conditions. These results suggest that conditions of oxidative stress increased the Hsp27 and Hsp70 protein levels.

Innate Immune System at the Maternal–Fetal Interface: Mechanisms of Disease and Targets of Therapy in Pregnancy Syndromes

“…82 Recently, a higher expression of Hsp70 and Hsp27 was documented in the maternal serum of PE women than that in the normal pregnancy. 83 …”

Study of Combination of Nanoherbal Andaliman (Zanthoxylum acanthopodium) and Extra Virgin Olive Oil (EVOO) Effects in the Expression of Malondialdehyde (MDA), Heat Shock Protein-70 (HSP70) and Placental Histology of Preeclamptic Rats

“…Pre-eclampsia (PE) is one of the major causes of morbidity and maternal and perinatal mortality, with characteristics such as hypertension and proteinuria in pregnancies over 20 weeks and complex disorders involving the maternal and placental components. 1,2 According to the Indonesian Demographic and Health Survey, PE contributed to 24% of pregnant women and is the second cause of maternal death in Indonesia. 3 This disease is one of the most complicated hypertensive disorders during pregnancy.…”

Rekombinan Vascular Endothelial Growth Factor-121 Menekan Ekspresi Heat Shock Protein-70 pada Plasenta Mencit Model Preeklampsia (VASCULAR ENDOTHELIAL GROWTH FACTOR-121 RECOMBINANT DEPRESSIS HEAT SHOCK PROTEIN-70 EXPRESSION IN PREECLAMPSIA MICE PLACENTA

“…Preeklampsia merupakan penyebab utama morbiditas dan mortalitas maternal maupun perinatal dengan karakteristik adanya hipertensi dan proteinuria pada usia kehamilan di atas 20 minggu dan terdapat kelainan yang kompleks termasuk komponen maternal dan plasental (Sahin et al, 2015;Sheikhi et al, 2015). Ghulmiyyah dan Sibai (2012) menyatakan bahwa preeklampsia merupakan sindrom klinis dengan karakteristik onset baru dari hipertensi dan proteinuria setelah 20 minggu usia kehamilan/gestasi pada wanita yang sebelumnya normotensi.…”

“…Soluble Fms-like tyrosine kinase-1 merupakan antagonis dari PlGF dan VEGF yang terikat pada reseptor yang sama. Konsentrasi PlGF dan VEGF menurun pada preeklampsia (Sahin et al, 2015;Creasy et al, 2014;Tal, 2012). Kadar sFlt-1 meningkat secara gradual pada preeklampsia antara 25-28 minggu dan meningkat 5-6 minggu sebelum onset preeklampsia (Muller dan Schiffer, 2014).…”

“…Stres oksidatif menyebabkan meningkatnya kadar HSP-70 yang merupakan respons dari stres protein. Pada preeklampsia adanya stres protein pada plasenta menyebabkan HSP-70 meningkat ekspresinya untuk memproteksi dan mempertahankan kehidupan sel dari apoptosis karena stress (Sahin et al, 2015). Senyawa HSP-70 sangat tinggi kadarnya pada preeklampsia early onset.…”