Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors

Abstract

Breast cancer is the most common type of cancer among women worldwide. Research using breast cancer cell lines derived from primary tumors may provide valuable additional knowledge regarding this type of cancer. Therefore, the aim of this study was to investigate the phenotypic profiles of MACL-1 and MGSO-3, the only Brazilian breast cancer cell lines available for comparative studies. We evaluated the presence of hormone receptors, proliferation, differentiation and stem cell markers, using immunohistochemical staining of the primary tumor, cultured cells and xenografts implanted in immunodeficient mice. We also investigated the ability of the cell lines to form colonies and copy number alterations by array comparative genomic hybridization. Histopathological analysis showed that the invasive primary tumor from which the MACL-1 cell line was derived, was a luminal A subtype carcinoma, while the ductal carcinoma in situ (DCIS) that gave rise to the MGSO-3 cell line was a HER2 subtype tumor, both showing different proliferation levels. The cell lines and the tumor xenografts in mice preserved their high proliferative potential, but did not maintain the expression of the other markers assessed. This shift in expression may be due to the selection of an ‘establishment’ phenotype in vitro. Whole-genome DNA evaluation showed a large amount of copy number alterations (CNAs) in the two cell lines. These findings render MACL-1 and MGSO-3 the first characterized Brazilian breast cancer cell lines to be potentially used for comparative research.

Translocation of Intracellular CD24 Towards Cell Membrane Constitutes a Triggering Event for Drug Resistance in Breast Tumor Cell: Correlation With Dynamics of p38MAPK Activation

“…Concerning MACL-1 and MGSO-3 breast cell lines, obtained from Brazilian patients, the percentage of CD24 + cells was about 5% and 46% respectively, corroborating with their classi cation in other study [24]. The presence of intracellular CD24 was also detected in the whole population of both cell lines, like observed in MDA-MB-231 population (Fig 2a,b).…”

Comparative genomic hybridization analysis of invasive ductal breast carcinomas in the Chinese population

“…Several studies have provided evidence that loss or deletion on chromosome 17p12-13 is associated with chemotherapy resistance in breast cancer, as well as esophageal, lung, liver and stomach cancer ( 1 , 2 , 5 , 7 – 10 ). A study performed in Brazil that investigated only two patients demonstrated loss of considerable portions of chromosomes 17, 19 and 22 ( 18 ). The findings of this previous study are consistent with the present results.…”

Flash from the Back; Molecular and Epidemiological aspects of Breast Cancer in Last 15 Years

“…This condition is caused by the low levels of BRCA-2 protein in cells. This lead to the accumulation of damaged DNA in cell [8].…”

“…ILC also called as infiltrating lobular carcinoma, this carcinoma is the second most common type of breast cancer after IDC, according to American society of cancer; more than 180,000 women in US having ILC each year. This carcinoma spread to the adjacent tissues and begin in milk producing lobules which empty out in the duct that carry the milk to nipple [8].…”