Genomic and Phenotypic Evolution of Achromobacter xylosoxidans during Chronic Airway Infections of Patients with Cystic Fibrosis

Khademi, Seyed Mohammad Hossein; Gabrielaitė, Miglė; Paulsson, Magnus; Knulst, Mattis; Touriki, Eleni; Marvig, Rasmus L.; Påhlman, Lisa I.

doi:10.1128/msystems.00523-21

Cited by 15 publications

(13 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Nielsen et al ( 2019) have found that inactivation of the agellar M-ring protein, FliF, in A. xylosoxidans resulted in a 39% reduction of in vitro bio lm formation on peg-lids. Moreover, Khademi et al (2021) showed that the swimming motility of A. xylosoxidans is gradually lost over time following the initial colonization.…”

Section: Bio Lm Forming Capacity and Motilitymentioning

confidence: 99%

Isolation And Characterization Of A Newly Chrysene-Degrading A. aegrifaciens

Lazzem

Lekired

Ouzari

et al. 2022

Preprint

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are being considered as substances of potential human health hazards due to their resistance toward biodegradation and carcinogenic index. Chrysene is a high molecular weight (HMW)PAHs; the biodegradation of Chrysene using bacteria is recognized as an efficient, environmentally friendly, and cost-effective approach. In our study, a potential chrysene degrading bacterial strain Achromobacter (A.) aegrifaciens was isolated from crude oil-contaminated seawater. A. aegrifaciens was capable of utilizing Chrysene as sole sources of carbon and energy by showing 86% chrysene degradation on day 7, under the conditions of pH 7.0, temperature 30°C, and rotary speed 120 rpm. Bacterial counts have reached a maximum level (over 25 × 1032 CFU/mL). Furthermore, the results revealed that A. aegrifaciens produced biofilm on polystyrene at 20°Cand 30°C, as well as in the air-liquid (A-L) interface. Moreover, A. aegrifaciens were able to swim and to produce biosurfactant with an emulsification activity (E24%) over 10%. Based on the above results, A. aegrifaciens can thus be predicted as a potential candidate for bioremediation of HMW PAHs impacted environments.

show abstract

Section: Bio Lm Forming Capacity and Motilitymentioning

confidence: 99%

Isolation And Characterization Of A Newly Chrysene-Degrading A. aegrifaciens

Lazzem

Lekired

Ouzari

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…During colonization or infection, bacterial populations experience selection to adapt to different host niches 9 . Increased biofilm capacity is often found as a within-host adaptation throughout prolonged chronic infections, for example, for Pseudomonas aeruginosa and other bacteria in cystic fibrosis lungs 10,11 . Since biofilm communities embedded in extracellular matrix are much more recalcitrant to antibiotic treatment and immune factors 1 , they do not experience the same host-related bottlenecks as non-biofilm populations.…”

Section: Introductionmentioning

confidence: 99%

Rapid evolution ofKlebsiella pneumoniaebiofilmsin vitrodelineates adaptive changes selected during infection

Zaborskytė,

Coelho,

Wrande

et al. 2024

Preprint

View full text Add to dashboard Cite

Biofilm formation facilitates infection by the opportunistic pathogenKlebsiella pneumoniae, primarily through indwelling medical devices. Here, we address howK. pneumoniaecan increase its biofilm capacity by experimental evolution of surface-attached biofilms to mimic catheter-associated infections. We observed rapid convergent evolution that altered or abolished capsule, modified the fimbrial adhesin MrkD, or increased production of fimbriae and cellulose via upregulated c-di-GMP-dependent pathways. However, evolutionary trajectories and resulting phenotypes showed strain differences, illustrating the importance of genetic background on biofilm adaptation. Multiple biofilm aspects, such as early attachment, biofilm topology, surface preference, and extracellular matrix composition, were affected in a mutation-specific manner. Acute virulence was linked to the underlying genetic change rather than the overall biofilm capacity. Single mutations conferring hypermucoidy and c-di-GMP-related changes extensively overlapped with previously identified adaptive changes in UTI and wound isolates, confirming biofilm as an important selective traitin vivo.

show abstract

“…Recent studies have highlighted the cytotoxicity of A. xylosoxidans for murine macrophages based on the potential cytotoxic role of the T3SS ubiquitin-activated phospholipase effector AxoU ( 26 ) and a giant repeats-in-toxin (RTX) adhesin ( 27 ). Comparative genomic studies of A. xylosoxidans isolated from acute and chronic infections indicate that the bacteria can adapt to chronic lung infection, as evidenced by increased antimicrobial resistance, decreased motility, alteration in biofilm formation, and reduced cytotoxicity ( 26 , 28 – 31 ).…”

Section: Introductionmentioning

confidence: 99%