Genomic and clinical evidence uncovers the enterohepatic species <i>Helicobacter valdiviensis</i> as a potential human intestinal pathogen

Fresia, Pablo; Jara, Ronald; Sierra, Rafael Tojo; Ferrés, Ignacio; Greif, Gonzalo; Iraola, Gregorio; Collado, Luís

doi:10.1111/hel.12425

Cited by 14 publications

(14 citation statements)

References 25 publications

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“…Although EHH are frequently detected in clinical samples by PCR (Fox, ; Mateos‐Muñoz et al, ; Fresia et al, ), only occasionally they are isolated using medium and atmospheric conditions designed for Campylobacter spp. According to the Bergey’s Manual, there is no ideal method for the isolation of EHH and the recovery of these species is often optimized by the simultaneous use of selective and nonselective procedures (On et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…Enterohepatic species of the genus Helicobacter (EHH) have become increasingly important as emerging pathogens (Collado et al, 2014;Fresia et al, 2017) due to their association with acute gastroenteritis, inflammatory bowel disease (Crohn's disease and ulcerative colitis) and liver, gallbladder and bile ducts diseases (Man et al, 2008;Martel et al, 2009;Bascuñana et al, 2011;Thomson et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml−1. Our findings suggest that Columbia or Brucella media, regardless of the use of hydrogen, can be used for the EHH isolation. In addition, the concentration of antibiotics included in commercial campylobacteria supplements is suitable for EHH species recovery. Significance and Impact of the Study Enterohepatic Helicobacter (EHH) infections have been associated with several diseases in humans such as acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases. Although they are frequently detected in clinical samples by molecular methods, only occasionally they are isolated using culture conditions described for the taxonomic related pathogen Campylobacter sp. This is because the optimal conditions for the isolation of EHH have not yet been described, which results in an underestimation of the prevalence and clinical importance of these emerging pathogens. Therefore, this study provides insight for culturing EHH species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Ochoa

Martínez

Fernández

et al. 2019

Lett Appl Microbiol

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“…Another genome sequencing study, coming from Chile and Uruguay, compared the genome of Helicobacter valdiviensis to other Helicobacter species. The study showed that H. valdiviensis belongs to a monophyletic clade formed by Helicobacter canadensis , Helicobacter pullorum , Helicobacter winghamensis , Helicobacter rodentium , and Helicobacter apodemus . Additional PCR analyses revealed the presence of H. valdiviensis in some of stools samples of patients with acute diarrhea suggesting a potential role as an intestinal pathogen.…”

Section: Taxonomy Prevalence and Diagnosis Of Non‐helicobacter Pylomentioning

confidence: 88%

“…The study showed that H. valdiviensis belongs to a monophyletic clade formed by Helicobacter canadensis, Helicobacter pullorum, Helicobacter winghamensis, Helicobacter rodentium, and Helicobacter apodemus. 3 Additional PCR analyses revealed the presence of H. valdiviensis in some of stools samples of patients with acute diarrhea suggesting a potential role as an intestinal pathogen. Another interesting study looked at the microbiome composition in coelomic fluid of marine invertebrates.…”

Section: Ta Xonomy Pre Valen Ce and D Iag Nos Is Of Non -Helicobmentioning

confidence: 98%

Other Helicobacters and the gastric microbiome

Kupčinskas

Hold

2018

Helicobacter

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The current article is a review of the most important, accessible, and relevant literature published between April 2017 and March 2018 on other Helicobacters and the gastric microbiome. The first part of the review focuses on literature describing non-Helicobacter pylori-Helicobacter (NHPH) infections in humans and animals whilst the subsequent section focuses specifically on the human gastric microbiome. Novel diagnostic methods as well as new NHPHs species have been identified in recent studies. Furthermore, our knowledge about the pathogenesis of NHPH infections has been further enhanced by important fundamental studies in cell lines and animal models. Over the last year, additional insights over the prevalence and potential prevention strategies of NHPHs have also been reported. With regard to understanding the gastric microbiome, new information detailing the structure of the gastric microbiota at different stages of H. pylori infection, within different patient geographical locations, was documented. There was also a study detailing the impact of proton-pump inhibitor usage and the effect on the gastric microbiome. Newer analysis approaches including defining the active microbiome through analysis of RNA rather than DNA-based sequencing were also published allowing the first assessments of the functional capabilities of the gastric microbiome.

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“…For example, the H. pylori cag pathogenicity island is intimately associated with increased potential to cause more severe pathology, including cancer and gastric ulcers (25). There were five fragments with anomalous GC content in the H. himalayensis genome, and the predicted genome These helicobacters with the term "enterohepatic helicobacters (EHH)" colonize the intestinal mucosa or the liver, and are associated with chronic liver or intestinal inflammation (27). Recently, many cases of EHH infection in humans were reported,…”

Section: Discussionmentioning

confidence: 99%

Genomic and Histological Analysis of Helicobacter Himalayensis Sp. Nov. Isolated From Marmota Himalayana to Discovery the Potential Human Pathogen

et al. 2020

Preprint

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Background: Helicobacter himalayensis was isolated from Marmota himalayana in the Qinghai-Tibet Plateau, China, and is a new non-H. pylori species, with unclear taxonomy, phylogeny, and pathogenicity. Methods and results: A comparative genomic analysis was performed between the H. himalayensis type strain 80(YS1) and other the genomes of Helicobacter species present in the National Center for Biotechnology Information (NCBI) database to explore the molecular evolution and potential pathogenicity of H. himalayensis. We also compared the lesions of gastrointestinal tissue samples from H. himalayensis-positive Marmota himalayana with H. himalayensis-negative Marmota himalayana using histology. H. himalayensis 80(YS1)T formed a clade with H. cinaedi and H. hepaticus that was phylogenetically distant from H. pylori. The genome of 80(YS1)T comprised 1,829,936 bp, with a 39.9% GC content, a predicted genomic island, and 1,910 predicted proteins. Comparatively, H. himalayensis has more genes for functions in “cell wall/membrane/envelope biogenesis” and “nucleotide transport and metabolism” sub-branches than the other compared helicobacters, and its genome contained 42 virulence factors genes, including that encoding cytolethal distending toxin (CDT). Histologically, the gastrointestinal mucosa of H. himalayensis-infected Marmota himalayana showed obvious lesions, implying that H. himalayensis is pathogenic to its host. Conclusions: We characterized the H. himalayensis 80(YS1)T genome, its phylogenetic position, and its potential pathogenicity. 80(YS1)T may be pathogenic to animals or humans; however, further understanding of the pathogenesis of this potentially pathogenic bacterium is required, which might help to manage H. himalayensis-induced diseases.

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Genomic and clinical evidence uncovers the enterohepatic species Helicobacter valdiviensis as a potential human intestinal pathogen

Cited by 14 publications

References 25 publications

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Comparison of media and growth conditions for culturing enterohepaticHelicobacterspecies

Other Helicobacters and the gastric microbiome

Genomic and Histological Analysis of Helicobacter Himalayensis Sp. Nov. Isolated From Marmota Himalayana to Discovery the Potential Human Pathogen

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