Abstract:The structure of wall-anchored glycopolymers wall teichoic acid (WTA) produced by most Gram-positive bacteria is highly variable. While most dominant
Staphylococcus aureus
lineages produce poly-ribitol-phosphate (RboP) WTA, the
tagN, tarM
-encoding ST630 lineage probably has a poly-glycerol-phosphate (GroP) WTA backbone like coagulase-negative staphylococci (CoNS).
“…show that type III-A CRISPR-cas systems are present in more than half of the examined strains belonging to the emerging clone, ST630 [21,22]. As in previous studies, the CRISPR-cas system is located within the SCCmec cassette type V(5C2&5) and has substantial homology to similar elements in the CoNS.…”
Section: Introductionsupporting
confidence: 70%
“…The most prevalent CRISPR + S. aureus clone is ST630 with more than half of the isolates carrying CRISPR- cas , followed by ST1, ST5 and ST130 (Table 1). As ST630 is an emerging clone in both Denmark and China [21, 22, 25], we decided to include 30 additional ST630 isolates sequenced after 12 th of March 2019. We furthermore excluded a few isolates due to inadequate CRISPR array sequencing, bringing the total number of CRISPR + strains to 69.…”
Section: Resultsmentioning
confidence: 99%
“…Indeed, we observed that the S. psuedointermedius SCC mec containing CRISPR- cas has an identity of 99% to that of strain 110900. The occurrence of highly conserved SCC mec elements containing CRISPR- cas in strains of sequence type ST630 could be related to their unusual composition of cell wall teichoic acids that has been proposed to enable horizontal gene transfer between coagulase-negative staphylococci and S. aureus [21].…”
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-cas, however in the human pathogen Staphylococcus aureus, CRISPR-cas loci are less common and often studied in heterologous systems. We analyzed the prevalence of CRISPR-cas in genomes of methicillin resistant Staphylococcus aureus (MRSA) isolated in Denmark. Only 2.9 % of the strains carried CRISPR-cas systems, but for strains of sequence type ST630 over half were positive. All CRISPR-cas loci were type III-A and located within the staphylococcal chromosomal cassette (SCCmec) type V(5C2&5) conferring β-lactam resistance. Curiously, only 23 different CRISPR spacers were identified in 69 CRISPR-positive strains and almost identical SCCmec cassettes, CRISPR arrays and cas genes, are present in staphylococcal species other than aureus, suggesting that these were transferred horizontally. For the ST630 strain 110900, we demonstrate that the SCCmec cassette containing CRISPR-cas excises from the chromosome at high frequency. However, the cassette was not transferable under the conditions investigated. One of the CRISPR spacers targets a late gene in the lytic bacteriophage (phage) virus phiIPLA-RODI, and we show that the system protects against phage infection by reducing phage burst size. However, CRISPR-Cas can be overloaded or bypassed by CRISPR escape mutants. Our results imply that the endogenous type III-A CRISPR-Cas system in S. aureus is active against targeted phages, albeit with low efficacy. This suggests native S. aureus CRISPR-Cas offers only partial immunity, and in nature may work in tandem with other defence systems.
“…show that type III-A CRISPR-cas systems are present in more than half of the examined strains belonging to the emerging clone, ST630 [21,22]. As in previous studies, the CRISPR-cas system is located within the SCCmec cassette type V(5C2&5) and has substantial homology to similar elements in the CoNS.…”
Section: Introductionsupporting
confidence: 70%
“…The most prevalent CRISPR + S. aureus clone is ST630 with more than half of the isolates carrying CRISPR- cas , followed by ST1, ST5 and ST130 (Table 1). As ST630 is an emerging clone in both Denmark and China [21, 22, 25], we decided to include 30 additional ST630 isolates sequenced after 12 th of March 2019. We furthermore excluded a few isolates due to inadequate CRISPR array sequencing, bringing the total number of CRISPR + strains to 69.…”
Section: Resultsmentioning
confidence: 99%
“…Indeed, we observed that the S. psuedointermedius SCC mec containing CRISPR- cas has an identity of 99% to that of strain 110900. The occurrence of highly conserved SCC mec elements containing CRISPR- cas in strains of sequence type ST630 could be related to their unusual composition of cell wall teichoic acids that has been proposed to enable horizontal gene transfer between coagulase-negative staphylococci and S. aureus [21].…”
CRISPR-Cas is an adaptive immune system that allows bacteria to inactivate mobile genetic elements. Approximately 50% of bacteria harbor CRISPR-cas, however in the human pathogen Staphylococcus aureus, CRISPR-cas loci are less common and often studied in heterologous systems. We analyzed the prevalence of CRISPR-cas in genomes of methicillin resistant Staphylococcus aureus (MRSA) isolated in Denmark. Only 2.9 % of the strains carried CRISPR-cas systems, but for strains of sequence type ST630 over half were positive. All CRISPR-cas loci were type III-A and located within the staphylococcal chromosomal cassette (SCCmec) type V(5C2&5) conferring β-lactam resistance. Curiously, only 23 different CRISPR spacers were identified in 69 CRISPR-positive strains and almost identical SCCmec cassettes, CRISPR arrays and cas genes, are present in staphylococcal species other than aureus, suggesting that these were transferred horizontally. For the ST630 strain 110900, we demonstrate that the SCCmec cassette containing CRISPR-cas excises from the chromosome at high frequency. However, the cassette was not transferable under the conditions investigated. One of the CRISPR spacers targets a late gene in the lytic bacteriophage (phage) virus phiIPLA-RODI, and we show that the system protects against phage infection by reducing phage burst size. However, CRISPR-Cas can be overloaded or bypassed by CRISPR escape mutants. Our results imply that the endogenous type III-A CRISPR-Cas system in S. aureus is active against targeted phages, albeit with low efficacy. This suggests native S. aureus CRISPR-Cas offers only partial immunity, and in nature may work in tandem with other defence systems.
“…The great electrical conductivity of Ti 3 C 2 T x films has been confirmed (Ti 3 C 2 T x monolayer: 4600 ± 1100 S/cm) ( Ling et al, 2014 ; Lipatov et al, 2019 ; Tian et al, 2019 ), which is similar to or even exceeds that of reduced graphene oxide. The main component of the Gram (+) bacterial cell wall is peptidoglycan (PG), which is composed of the anionic sugar polymer wall called teichoic acid (WTA) ( Xiong et al, 2022 ). These WTAs are critical in maintaining bacterial structure, replication, and other major cellular functions ( Silhavy et al, 2010 ; Rohde, 2019 ).…”
Background: Two-dimensional(2D)MXenes have continued to receive increasing interest from researchers due to their graphene-like properties, in addition to their versatile properties for applications in electronic devices, power generation, sensors, drug delivery, and biomedicine. However, their construction and biological properties as titanium coatings to prevent peri-implantitis are still unclear.Materials and methods: In this work, few-layer Ti3C2Tx MXene coatings with different thicknesses at varied depositing voltages (30, 40, and 50 V) were constructed by anodic electrophoretic deposition without adding any electrolytic ions. In vitro cytocompatibility assay was performed on preosteoblasts (MC3T3-E1) cell lines after the characterization of the coating. Meanwhile, the antibacterial activity against bacteria which are closely related to peri-implantitis including Staphylococcus aureus (S. aureus) and its drug-resistant strain MRSA was further investigated.Results: MXene-coated titanium models with different thicknesses were successfully assembled by analyzing the results of characterization. The compounding of Ti3C2Tx could significantly improve the initial adhesion and proliferation of MC3T3-E1 cells. Moreover, the coating can effectively inhibit the adhesion and cell activity of S. aureus and MRSA, and MRSA expressed greater restricting behavior than S. aureus. The ability to promote antibacterial activity is proportional to the content of Ti3C2Tx. Its antioxidant capacity to reduce ROS in the culture environment and bacterial cells was first revealed.Conclusion: In summary, this work shows a new avenue for MXene-based nano-biomaterials under the clinical problem of multiple antibiotic resistance.
“…In the present study, sporadic MRSA isolates of ST1, ST6, ST9, ST15, ST22, ST25, ST72, ST338 (a single-locus variant of ST59), ST398, ST630, and ST1281 were also identified. WGS-based genomic epidemiology of some of the aforementioned MRSA STs has been studied ( 19 , 30 – 34 ). The continued emergence of new MRSA clones/isolates in hospitals further emphasizes the importance of timely surveillance.…”
Staphylococcus aureus
is an important bacterium pathogen in tertiary hospitals, which provide rich medical resources. Tianjin is one of the four municipalities in China with a population of more than 13 million.
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