Genomic analysis of early murine mammary gland development using novel probe-level algorithms

Master, Stephen R.; Stoddard, Alexander J.; Pan, Tien-Chi; Dugan, Katherine D.; Chodosh, Lewis A.

doi:10.1186/gb-2005-6-2-r20

Cited by 16 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We assayed three biological replicates per genotype. cRNAs were generated by in vitro transcription and probed against the Affymetrix Gene Chip and detected probes were evaluated using two types of analysis platforms (MAS 5 and ChipStat, see Methods and (Master et al, 2005; Wang et al, 2006)) to determine differences in gene expression between the mutants and the wild-type control.…”

Section: Resultsmentioning

confidence: 99%

“…Gene expression analysis was performed as previously described using Affymetrix Microarray Suite MAS 5.0 (Affymetrix) and ChipStat, which detects significant gene expression changes even when fold change is small (Master et al, 2005; Wang et al, 2006). Briefly, ChipStat utilizes the number of individual probe pairs that differ between conditions (certainty of p < 0.05) to determine positives, even if the fold-change is very small, for at least 6 of 14 probe pairs.…”

Section: Methodsmentioning

confidence: 99%

“…MAS 5.0 performs 9 possible pairwise comparisons and ranks significance of overall fold changes. Prior studies with these methods had a false positive rate of <1.5% (Master et al, 2005; Wang et al, 2006). Other studies also support utilizing multiple analysis methods, for example (Dalman et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Manning

Sheth

Bridges

et al. 2017

Mechanisms of Development

View full text Add to dashboard Cite

Cell migration is essential during animal development. In the Drosophila ovary, the steroid hormone ecdysone coordinates nutrient sensing, growth, and the timing of morphogenesis events including border cell migration. To identify downstream effectors of ecdysone signaling, we profiled gene expression in wild-type follicle cells compared to cells expressing a dominant negative Ecdysone receptor or its coactivator Taiman. Of approximately 400 genes that showed differences in expression, we validated 16 candidate genes for expression in border and centripetal cells, and demonstrated that seven responded to ectopic ecdysone activation by changing their transcriptional levels. We found a requirement for seven putative targets in effective cell migration, including two other nuclear hormone receptors, a calcyphosine-encoding gene, and a prolyl hydroxylase. Thus, we identified multiple new genetic regulators modulated at the level of transcription that allow cells to interpret information from the environment and coordinate cell migration in vivo.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Manning

Sheth

Bridges

et al. 2017

Mechanisms of Development

View full text Add to dashboard Cite

show abstract

“…The onset of puberty initiates major changes in the female mammary gland, both in the gene expression [11] and morphology [7]. The two ovarian hormones, E and P, acting through their respective receptors, stimulate allometric growth and morphogenesis of the gland [7, 12].…”

Section: Key Stages and Hormones In Mammary Gland Developmentmentioning

confidence: 99%

Can Modulation of Mammary Gland Development by Dietary Factors Support Breast Cancer Prevention?

Wärri

Saarinen²,

Mäkelä³

2007

Horm Res Paediatr

View full text Add to dashboard Cite

Breast cancer continues to be a major challenge for public health, since it is the most common cancer of women in the Western world, and its prevalence is still increasing. In order to achieve better results in the prevention and treatment of breast cancer it is crucial to identify the mechanisms behind its initiation, i.e. the changes and deviations that have occurred in the mammary gland growth. It has long been known that a woman’s reproductive history is the strongest breast cancer risk factor if genetic background and age are excluded. The reproductive hormones, and the timing of events leading to changes in these hormones, and consequently, in the mammary gland, are the most important players. However, it has become obvious that dietary components may also contribute to breast cancer risk through their effects on the mammary gland. The past few years have added important information to our knowledge of the mechanisms behind breast cancer initiation at the level of target cells (mammary stem cells) and gene expression (genetic ‘fingerprint’ associated with persistent pregnancy-induced protection against breast cancer), as well as of the effects of certain dietary factors (steroid action modulators). These results and their links to breast cancer initiation and progression will be discussed.

show abstract

Profile of estrogen‐responsive genes in an estrogen‐specific mammary gland outgrowth model

Deroo

Hewitt

Collins

et al. 2009

Molecular Reproduction Devel

View full text Add to dashboard Cite

Both ovarian and pituitary hormones are required for the pubertal development of the mouse mammary gland. Estradiol directs ductal elongation and branching, while progesterone leads to tertiary branching and alveolar development. The purpose of this investigation was to identify estrogen-responsive genes associated with pubertal ductal growth in the mouse mammary gland in the absence of other ovarian hormones and at different stages of development. We hypothesized that the estrogen-induced genes and their associated functions at early stages of ductal elongation would be distinct from those induced after significant ductal elongation had occurred. Therefore, ovariectomized prepubertal mice were exposed to 17β-estradiol from two to twenty-eight days, and mammary gland global gene expression analyzed by microarray analysis at various times during this period. We found that: a) gene expression changes in our estrogen-only model mimic those changes that occur in normal pubertal development in intact mice, and b) both distinct and overlapping gene profiles were observed at varying extents of ductal elongation, and c) cell proliferation, the immune response, and metabolism/catabolism were the most common functional categories associated with mammary ductal growth. Particularly striking was the novel observation that genes active during carbohydrate metabolism were rapidly and robustly decreased in response to estradiol. Lastly, we identified mammary estradiol-responsive genes that are also co-expressed with Estrogen Receptor α in human breast cancer. In conclusion, our genomic data support the physiological observation that estradiol is one of the primary hormonal signals driving ductal elongation during pubertal mammary development.

show abstract

Genomic analysis of early murine mammary gland development using novel probe-level algorithms

Cited by 16 publications

References 45 publications

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

A hormonal cue promotes timely follicle cell migration by modulating transcription profiles

Can Modulation of Mammary Gland Development by Dietary Factors Support Breast Cancer Prevention?

Profile of estrogen‐responsive genes in an estrogen‐specific mammary gland outgrowth model

Contact Info

Product

Resources

About