Genome -wide transcriptome profiling in ovaries of small-tail Han sheep during the follicular and luteal phases of the oestrous cycle

Han, Wenling; Li, Xiangyun; Zhou, Rongyan; Xi, Jianzhong; Wei, Yan-Hui; Li, Lanhui; Zhang, Zhenhong

doi:10.1016/j.anireprosci.2018.08.031

Cited by 10 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, miR‐143 and miR‐99a was the most abundant miRNAs in the follicular and luteal phases of the Turpan black sheep. Additionally, miR‐99a and miR‐143 were also the most abundant in Small‐tail Han sheep (Wang et al., 2018) and goat (Zi et al., 2017) ovaries. MiRNA‐99a can regulate proliferation and apoptosis of human granulosa cells via targeting insulin‐like growth factor 1 ( IGF‐1R ) (Geng et al., 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Envidences show that miRNA regulates ovarian follicle development, granulosa cell proliferation and apoptosis, and steriod hormone synthesis and secretion. Through high‐throughput sequencing technology, a large number of miRNAs have been identified in ovaries of multiple species, including human (Andrei et al., 2018), mouse (Alexandri et al., 2019), sheep (Wang et al., 2018), goat (Zi et al., 2017), bovine (Gilbert et al., 2011), porcine (Zhang et al., 2018) and chicken (Wu et al., 2017). In our study, miR‐143 and miR‐99a was the most abundant miRNAs in the follicular and luteal phases of the Turpan black sheep.…”

Section: Discussionmentioning

confidence: 99%

“…The analysis method and protocol of miRNA sequencing and mRNA sequencing data referred our previous study (Wang et al., 2018). The custom Perl and Python scripts were used to remove adapter sequences and low‐quality reads.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, numerous researches have obtained miRNA expression profiles in ovary, for example, human, mice, pig and bovine (Maalouf et al., 2016; Schneider et al., 2017; Song et al., 2018; Xu et al., 2016). MiRNA expression levels present significant differences in luteal and follicular ovaries of hircine and prolific sheep, which indicates that miRNAs have an essential effect on the process of follicle to luteum transformation (Ling et al., 2016; Wang et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Song

Yue

et al. 2020

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

To investigate the regulatory mechanism of the follicular–luteal phase transition in Turpan black sheep (Ovis aries), the genome‐wide expression patterns of microRNAs (miRNAs) and genes were investigated in ovaries of six sheep (3 years and single lamb with 3 consecutive births) during follicular and luteal phases of the oestrous cycle. Bioinformatic analysis was used to screen potential miRNAs and genes related to Turpan black sheep ovarian function. RT‐qPCR was used to validate the sequencing results. In total, we identified 139 known and 71 novel miRNAs in the two phases with miRNA‐seq, and a total of 19 miRNAs were significantly differentially expressed, of which 7 were up‐regulated and 12 were down‐regulated in the follicular phase compared with luteal phase. A total of 150 genes were significantly differentially expressed, including 63 up‐regulated and 87 down‐regulated in the follicular phase compared with the luteal phase by RNA‐seq data analysis. Those DEGs were significantly enriched in 103 GO terms and several KEGG pathways, including metabolic pathway, ovarian steroidogenesis, steroid hormone biosynthesis and oestrogen signalling pathway. In addition, we created a miRNA–mRNA regulatory network to further elucidate the mechanism of follicular–luteal transition. Finally, we identified key miRNAs and genes including miR‐143, miR‐99a, miR‐150, miR‐27a, miR‐125b, STAR, STAT1, which might play crucial roles in reproductive hormone biosynthesis and follicular development. The miRNA–mRNA interactive network clearly illustrates molecular basis involving in follicular–luteal transition.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Song

Yue

et al. 2020

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several hundred miRNAs have been identified in small ruminant species; however, the functional roles of many of these miRNAs in the various regulatory systems remain unclear. Studies in sheep and goats have often focused on economic traits such as milk, meat, and wool production (82,(89)(90)(91)(92), and relatively few studies have investigated their health status related to welfare conditions (Figure 2 and Supplementary Table 2).…”

Section: Mirnas Related To Health and Welfare In Small Ruminantsmentioning

confidence: 99%

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

et al. 2020

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small and highly conserved non-coding RNA molecules that orchestrate a wide range of biological processes through the post-transcriptional regulation of gene expression. An intriguing aspect in identifying these molecules as biomarkers is derived from their role in cell-to-cell communication, their active secretion from cells into the extracellular environment, their high stability in body fluids, and their ease of collection. All these features confer on miRNAs the potential to become a non-invasive tool to score animal welfare. There is growing interest in the importance of miRNAs as biomarkers for assessing the welfare of livestock during metabolic, environmental, and management stress, particularly in ruminants, pigs, and poultry. This review provides an overview of the current knowledge regarding the potential use of tissue and/or circulating miRNAs as biomarkers for the assessment of the health and welfare status in these livestock species.

show abstract

miR-200b/MYBL2/CDK1 suppresses proliferation and induces senescence through cell cycle arrest in ovine granulosa cells

et al. 2023

View full text Add to dashboard Cite

Genome -wide transcriptome profiling in ovaries of small-tail Han sheep during the follicular and luteal phases of the oestrous cycle

Cited by 10 publications

References 28 publications

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

Integrated analysis of miRNA–mRNA interaction in ovaries of Turpan Black Sheep during follicular and luteal phases

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

miR-200b/MYBL2/CDK1 suppresses proliferation and induces senescence through cell cycle arrest in ovine granulosa cells

Contact Info

Product

Resources

About