Genome-Wide Survey and Expression Analysis of Amino Acid Transporter Gene Family in Rice (Oryza sativa L.)

Zhao, Hui; Ma, Hongwei; Yu, Li; Wang, Xin; Jie, Zhao

doi:10.1371/journal.pone.0049210

Cited by 155 publications

(223 citation statements)

References 70 publications

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“…8, A and B). We further compared the expression of genes possibly involved in the transfer of amino acids to the seeds of wild-type and pPP2:: AtSUC2 plants Zhao et al, 2012). Our results show that the expression of only encoding rice amino acid transporter7 (OsAAP7) was up-regulated in transgenic plants (Fig.…”

Section: Seed Carbon Metabolism and Transport Processesmentioning

confidence: 90%

Enhanced sucrose loading improves rice yield by increasing grain size

Wang

Wen

et al. 2015

Plant Physiol.

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Yield in cereals is a function of grain number and size. Sucrose (Suc), the main carbohydrate product of photosynthesis in higher plants, is transported long distances from source leaves to sink organs such as seeds and roots. Here, we report that transgenic rice plants (Oryza sativa) expressing the Arabidopsis (Arabidopsis thaliana) phloem-specific Suc transporter (AtSUC2), which loads Suc into the phloem under control of the phloem protein2 promoter (pPP2), showed an increase in grain yield of up to 16% relative to wild-type plants in field trials. Compared with wild-type plants, pPP2::AtSUC2 plants had larger spikelet hulls and larger and heavier grains. Grain filling was accelerated in the transgenic plants, and more photoassimilate was transported from the leaves to the grain. In addition, microarray analyses revealed that carbohydrate, amino acid, and lipid metabolism was enhanced in the leaves and grain of pPP2::AtSUC2 plants. Thus, enhancing Suc loading represents a promising strategy to improve rice yield to feed the global population.

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Section: Seed Carbon Metabolism and Transport Processesmentioning

confidence: 90%

Enhanced sucrose loading improves rice yield by increasing grain size

Wang

Wen

et al. 2015

Plant Physiol.

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“…Next, these genes were analyzed against the Pfam database using Simple Modular Architecture Research Tool (SMART) to identify the presence of the conservative domains (Aa-trans and AApermease domains) of AAT proteins in Populus. After removing redundant sequences, a total of 100 AAT genes were identified in the Populus genome, which is greater than that identified in other representative species, including Arabidopsis (63), rice (Oryza sativa; 85), and maize (Zea mays; 96) (Rentsch et al 2007;Zhao et al 2012). Of the 100 AAT genes, 71 belong to the AAAP subfamily and 29 belong to the APC subfamily.…”

Section: Identification Of Aat Gene Family In P Trichocarpamentioning

confidence: 97%

“…AtAAP8 was expressed in young siliques and developing seeds and might play a crucial role in amino acid transport (Fischer et al 1995;Schmidt et al 2007). In addition, the AAP subfamily members from other species are also to be reported, such as StAAP1 (Koch et al 2003), PvAAP1 (Tan et al 2008), and OsAAP8 and OsAAP15 (Zhao et al 2012). Recently, it was proposed that PtAAP11 may play a major role in xylogenesis by providing proline in Popular (Couturier et al 2010).…”

Section: Communicated By a Brunnermentioning

confidence: 98%

Genome-wide survey and expression analysis of the amino acid transporter gene family in poplar

Chen

et al. 2015

Tree Genetics & Genomes

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Members of the amino acid transporters (AATs) gene family transported amino acid across cellular membranes and participated in various aspects of normal plant growth and developmental processes as well as environmental responses. To date, no overall analysis or expression profiling of the AAT gene family in Populus has been reported. An investigation of the Populus genome revealed 100 putative AAT genes. These genes were classified into 11 subfamilies based on phylogenetic analysis. In each subfamily, the constituent parts of gene structure and motif were relatively conserved. A total of 100 genes were distributed on 19 chromosomes with 18-pair segmental duplication and 19-gene tandem duplication events, indicating that segmental and tandem duplications contribute almost equally to the expansion of the PtAAT gene family. Analysis of the Ka/Ks ratios showed that the duplicated genes of the AAT family basically underwent purifying selection. The expression levels of the 17 amino acid/auxin permease (AAAP) subfamily genes under abiotic stresses and in different tissues were investigated by quantitative real-time PCR (qRT-PCR) to explore their stress-related and tissue-specific expression patterns. The qRT-PCR results to explore the precise role of individual PtAAT gene. This study presents a thorough overview of the Populus AAT gene family and provides a new perspective on the evolution of this gene family.The results indicate that AAT family genes may be involved in many plant responses to stress conditions. Additionally, this study provides a solid foundation for uncovering the biological roles of AAT genes.

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“…In addition, the mitochondrial GDH plays a major role in reassimilation of photorespiratory ammonia and can alternatively incorporate ammonium into Glu in response to high levels of ammonium under stress [72]. Although there are a large number of amino acid permeases (AAPs) presented in rice [74,75], no transporters have been functionally characterized with an exception for OsAAP6, which is mainly expressed in seeds for grain protein content [76]. Recently, the transport function of four rice AAP genes (OsAAP1, OsAAP3, OsAAP7, and OsAAP16) has been analyzed by expression in Xenopus laevis oocytes, electrophysiology, and cellular localization.…”

Section: Nitrogen Remobilization and Reassimilationmentioning

confidence: 99%

Nitrogen Use Efficiency in Rice

Huang¹,

Zhao²,

Zhang³

et al. 2018

Nitrogen in Agriculture - Updates

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Food security is a major global issue because of the growing population and decreasing land area. Rice (Oryza sativa L.) is the most important staple cereal crop in the world. Application of nitrogen (N) fertilizer has improved crop yield in the world during the past five decades but with considerable negative impacts on the environment. New solutions are therefore urgently needed to simultaneously increase yields while maintaining or preferably decreasing applied N to maximize the nitrogen use efficiency (NUE) of crops. Plant NUE is inherently complex with each step (including N uptake, translocation, assimilation, and remobilization) governed by multiple interacting genetic and environmental factors. Based on the current knowledge, we propose some possible approaches enhancing NUE, by molecular manipulation selecting candidate genes and agricultural integrated management practices for NUE improvement. Developing an integrated research program combining approaches, mainly based on whole-plant physiology, quantitative genetics, forward and reverse genetics, and agronomy approaches to improve NUE, is a major objective in the future.

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Genome-Wide Survey and Expression Analysis of Amino Acid Transporter Gene Family in Rice (Oryza sativa L.)

Cited by 155 publications

References 70 publications

Enhanced sucrose loading improves rice yield by increasing grain size

Enhanced sucrose loading improves rice yield by increasing grain size

Genome-wide survey and expression analysis of the amino acid transporter gene family in poplar

Nitrogen Use Efficiency in Rice

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