Genome-wide survey and expression analysis of the amino acid transporter gene family in poplar

Abstract

Members of the amino acid transporters (AATs) gene family transported amino acid across cellular membranes and participated in various aspects of normal plant growth and developmental processes as well as environmental responses. To date, no overall analysis or expression profiling of the AAT gene family in Populus has been reported. An investigation of the Populus genome revealed 100 putative AAT genes. These genes were classified into 11 subfamilies based on phylogenetic analysis. In each subfamily, the constituent parts of gene structure and motif were relatively conserved. A total of 100 genes were distributed on 19 chromosomes with 18-pair segmental duplication and 19-gene tandem duplication events, indicating that segmental and tandem duplications contribute almost equally to the expansion of the PtAAT gene family. Analysis of the Ka/Ks ratios showed that the duplicated genes of the AAT family basically underwent purifying selection. The expression levels of the 17 amino acid/auxin permease (AAAP) subfamily genes under abiotic stresses and in different tissues were investigated by quantitative real-time PCR (qRT-PCR) to explore their stress-related and tissue-specific expression patterns. The qRT-PCR results to explore the precise role of individual PtAAT gene. This study presents a thorough overview of the Populus AAT gene family and provides a new perspective on the evolution of this gene family.The results indicate that AAT family genes may be involved in many plant responses to stress conditions. Additionally, this study provides a solid foundation for uncovering the biological roles of AAT genes.

Differential Expression Pattern of Splice Variants of Amino Acid Transporter Genes from Rice Grown under Various Nitrogen Conditions and during Development

“…AAPs are highly regulated by environmental signals (Grallath et al, 2005). The PtAAT genes might play a critical role in abiotic stress signaling in Populus, because their expression was either increased or repressed after the PEG and cold treatments (Wu et al, 2015). However, little is known about the role of the OsAAT alternative splicing genes in response to environmental signal, especially N in the roots and leaves.…”

“…The AAT gene family members have been identified in Arabidopsis (Tegeder, 2012), rice (Lu et al, 2012), poplar (Wu et al, 2015), Solanum tuberosum L. (Ma et al, 2016) and Glycine max L. (Cheng et al, 2016). This showed that AAP transporters play an important role in loading of amino acids for nitrogen sink and supply (Tegeder and Ward, 2012).…”

Deciphering Genetic Architecture of Adventitious Root and Related Shoot Traits in Populus Using QTL Mapping and RNA-Seq Data

“…Three candidate genes Potri.004G111400, Potri.T021600, and Potri.012G082800 were identified in all above analysis. The gene PtAAAP19 (Potri.004G111400) encodes transmembrane amino acid transporter family protein [32], and its homologous gene in A. thaliana involves in adventitious root formation through the uptake of neutral and acidic amino acids in roots [33]. qRT-PCR analysis indicated that PtAAAP19 exhibited higher expression levels in fine rooting genotypes (ʹTongliao1ʹ, G1, G2, and G3) than poor rooting (ʹDanhongʹ, B1, B2, and B3) ( Figure 8C).…”

Comparative identification and differential expression pattern of amino acid permease genes in Elaeis guineensis

“…It was revealed that the C-repeat binding factor (CBF) mediated the gene expression pattern in E. guineensis under cold stress via RNA-Seq analysis [26]. Other reports indicated that the PtAAT genes might play a critical role in the abiotic stress signaling in Populus because their expression was either increased or repressed after the cold and PEG treatments [11]. However, little is known about the role of EgAAPs in response to environmental signals.…”

“…Furthermore, AAT families have also been identified in poplar [11], Solanum tuberosum L. [12] and Glycine max L. [13]. Importantly, studies have shown that amino acid permeases (AAPs), which belong to the AAT family [14][15][16], played an important role in loading amino acids for nitrogen sink and supply [17].…”