Genome wide microarray based expression profiles associated with Bm NPV resistance and susceptibility in Indian silkworm races of Bombyx mori

Lekha, Govindaraj; Gupta, Tania; Awasthi, A. K.; Murthy, Geetha N.; Trivedy, Kanika; Ponnuvel, Kangayam M.

doi:10.1016/j.ygeno.2015.09.002

Cited by 19 publications

(17 citation statements)

References 42 publications

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“…Studies on this virus thus far, have demonstrated its proliferation within the host through a cascade of viral gene expression and identifying host resistant genes against BmNPV infection [27–28]. However, the molecular mechanism of BmNPV-host interaction remains poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Jiang

Guo

et al. 2016

PLoS ONE

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Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Jiang

Guo

et al. 2016

PLoS ONE

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“…In the insect fat body, Tret1 transports trehalose to the hemolymph. Although trehalose and Tret1 are thought to be produced primarily in the insect fat body, transcripts with similarity to Tret1 are frequently expressed by insect midgut epithelium [88,89]. Interestingly, trehalose, which is hydrolyzed by trehalase to produce two glucose molecules, is the first sugar utilized in the insect chitin biosynthesis pathway [35,90].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Transcriptomic profiling of the digestive tract of the rat flea, Xenopsylla cheopis, following blood feeding and infection with Yersinia pestis

et al. 2020

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Yersinia pestis, the causative agent of plague, is a highly lethal pathogen transmitted by the bite of infected fleas. Once ingested by a flea, Y. pestis establish a replicative niche in the gut and produce a biofilm that promotes foregut colonization and transmission. The rat flea Xenopsylla cheopis is an important vector to several zoonotic bacterial pathogens including Y. pestis. Some fleas naturally clear themselves of infection; however, the physiological and immunological mechanisms by which this occurs are largely uncharacterized. To address this, RNA was extracted, sequenced, and distinct transcript profiles were assembled de novo from X. cheopis digestive tracts isolated from fleas that were either: 1) not fed for 5 days; 2) fed sterile blood; or 3) fed blood containing~5x10 8 CFU/ml Y. pestis KIM6+. Analysis and comparison of the transcript profiles resulted in identification of 23 annotated (and 11 unknown or uncharacterized) digestive tract transcripts that comprise the early transcriptional response of the rat flea gut to infection with Y. pestis. The data indicate that production of antimicrobial peptides regulated by the immune-deficiency pathway (IMD) is the primary flea immune response to infection with Y. pestis. The remaining infection-responsive transcripts, not obviously associated with the immune response, were involved in at least one of 3 physiological themes: 1) alterations to chemosensation and gut peristalsis; 2) modification of digestion and metabolism; and 3) production of chitin-binding proteins (peritrophins). Despite producing several peritrophin transcripts shortly after feeding, including a subset that were infection-responsive, no thick peritrophic membrane was detectable by histochemistry or electron microscopy of rat flea guts for the first 24 hours following blood-feeding. Here we discuss the physiological implications of rat flea infection-responsive transcripts, the function of X. cheopis peritrophins, and the mechanisms by which Y. pestis may be cleared from the flea gut.

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“…With the use of recently emerging data, researchers have begun to uncover the antiviral mechanisms in invertebrate immunity, particularly by screening for antiviral genes (Xia et al ., ; Bao et al ., ; Zhou et al ., ; Lekha et al ., ; Wang et al ., ; ; ; Xu et al ., ; Li et al ., ; Yu et al ., ). Whether these genes are resistance‐related genes remains unclear, however, and the exact antiviral mechanisms remain unknown.…”

Section: Discussionmentioning

confidence: 99%

“…We used Cuffquant and Cuffnorm components of cufflinks software to quantify the expression levels of transcripts and genes through the location information of mapped reads. We normalized Cuffquant and Cuffnorm via fragments per kilobase of transcript per million fragments mapped (FPKM) (Bao et al ., ; Lekha et al ., ; Wang et al ., ) using the formula FPKM = cDNA fragments/[mapped fragments (millions) × transcript length (kb)]. Genes with significant differences in the expression levels are referred to as DEGs or differentially expressed transcripts.…”

Section: Methodsmentioning

confidence: 99%

Specific genes related to nucleopolyhedrovirus in Bombyx mori susceptible and near‐isogenic resistant strains through comparative transcriptome analysis

Chen

Tan

et al. 2019

Insect Molecular Biology

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Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens that causes severe economic losses to sericulture. Comparative transcriptomics analysis has been widely applied to explore the antiviral mechanism in resistant strains. Here, to identify genes involved in BmNPV infection, we identified differentially expressed genes (DEGs) and performed weighted gene co‐expression network analysis (WGCNA) between two Bombyx mori strains: strain 871 (susceptible to BmNPV infection) and the near‐isogenic strain 871C (resistant to BmNPV). Our results showed that 400 genes were associated with resistance in strain 871C, and 76 genes were related to susceptibility in strain 871. In addition, the correlation analysis of DEGs and WGCNA showed that 40 genes related to resistance were highly expressed in the resistant strain. Among them, gene BGIBMGA004291 was the most noticeable. We further identified the effect of gene BGIBMGA004291, which encoded a multiprotein bridge factor 2 (MBF2) family member (MBF2‐10), on viral infection in cells. Our data suggested that MBF2‐10 inhibited viral infection. Taken together, this study showed specific module trait correlations related to viral infection in strains 871 and 871C, and we identified a resistance‐related gene. These findings suggested promising candidate genes with antiviral activity, aiding in the analysis of the antiviral molecular mechanisms in resistant strains.

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Genome wide microarray based expression profiles associated with Bm NPV resistance and susceptibility in Indian silkworm races of Bombyx mori

Cited by 19 publications

References 42 publications

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Transcriptomic profiling of the digestive tract of the rat flea, Xenopsylla cheopis, following blood feeding and infection with Yersinia pestis

Specific genes related to nucleopolyhedrovirus in Bombyx mori susceptible and near‐isogenic resistant strains through comparative transcriptome analysis

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