Genome-wide in silico identification and expression analysis of beta-galactosidase family members in sweetpotato [Ipomoea batatas (L.) Lam]

Abstract

Background Sweetpotato (Ipomoea batatas (L.) Lam.) serves as an important food source for human beings. β-galactosidase (bgal) is a glycosyl hydrolase involved in cell wall modification, which plays essential roles in plant development and environmental stress adaptation. However, the function of bgal genes in sweetpotato remains unclear. Results In this study, 17 β-galactosidase genes (Ibbgal) were identified in sweetpotato, which were classified into seven subfamilies using interspecific phylogenetic and comparative analysis. The promoter regions of Ibbgals harbored several stress, hormone and light responsive cis-acting elements. Quantitative real-time PCR results displayed that Ibbgal genes had the distinct expression patterns across different tissues and varieties. Moreover, the expression profiles under various hormonal treatments, abiotic and biotic stresses were highly divergent in leaves and root. Conclusions Taken together, these findings suggested that Ibbgals might play an important role in plant development and stress responses, which provided evidences for further study of bgal function and sweetpotato breeding.

Genome-wide identification, phylogeny and expression analysis of AP2/ERF transcription factors family in sweet potato

“…The sweet potato [ 37 ], which originated in Central America and belongs to the Convolvulaceae family, is an important food crop grown globally and has significant medicinal value [ 38 ]. Additionally, with the release of sweet potato genome data and the advancement of transgenic technology, it has become possible to identify and investigate important gene families at the whole genome level.…”

“…Additionally, with the release of sweet potato genome data and the advancement of transgenic technology, it has become possible to identify and investigate important gene families at the whole genome level. Beta-galactosidase family members of the sweet potato have been identified at the genome-wide level [ 37 ]. Additionally, genome-wide characterisations of several potassium absorption-related gene families, such as the HAK K + transport family [ 39 ] and the Shaker K + channel family [ 40 ], have also been investigated.…”

Molecular Characterization Reveals the Involvement of Calcium Dependent Protein Kinases in Abiotic Stress Signaling and Development in Chickpea (Cicer arietinum)

“…A total of 16 types of cis -regulatory elements were found to be variably distributed in CaCDPK promoters ( Figure 6 , Supplementary Table 3 ). These elements majorly belong to three categories; stress-responsive (GT1-motif, LTR, WUN-motif, ARE, TC-rich repeats, Box-4, AE-box, MYB, Myb-like, MYC, chs-CMA1a, and STRE), hormone-responsive (ABRE, ERE), and plant development related (AAGAA-motif, GATA-motif) (Hughes et al, 2000 ; Abe et al, 2003 ; Yamaguchi-Shinozaki and Shinozaki, 2005 ; Sharma et al, 2011 ; Nakashima and Yamaguchi-Shinozaki, 2013 ; Deepika and Singh, 2021 ; Hou et al, 2021 ). Abiotic stress-related motif Box-4 (light-responsive) was present in all CaCDPK promoters, and MYB motif (drought, low temperature, salt stress-related) was found in all CaCDPK promoters except CaCDPK2 .…”

BcWRKY33A Enhances Resistance to Botrytis cinerea via Activating BcMYB51-3 in Non-Heading Chinese Cabbage

“…Regarding MYB51 and its homologs, AtMYB51 was up-regulated after wound treatment [ 52 ]. In the functional prediction of promoters of BcMYB51s ( Figure S3 , Table S1 ), we found many generic elements including AE-box, AT1-motif, chs-CMA1/2a, GA-motif, GATA-motif, G-Box, and TCT-motif, indicating that BcMYB51s may respond to light signals [ 53 , 54 , 55 ]. Some phytohormone-related responsive elements were also found, such as AuxRR-core, CGTCA-motif, P-box, TATC-box, and TGAVG-motif, suggesting that plant hormones are involved in the regulation of BcMYB51s [ 56 , 57 ].…”