Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus

Wu, Xinyang; Lai, Yuchao; Rao, Shaofei; Lv, Lanqing; Ji, Mengfei; Han, Kelei; Weng, Jiajia; Lu, Yuwen; Peng, Jiejun; Lin, Lin; Wu, Guanwei; Chen, Jianping; Yan, Fei; Zheng, Hongying

doi:10.3389/fpls.2021.627315

Cited by 6 publications

(6 citation statements)

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“…p and HRLI: Potri.010G073400.1. p) (Lv et al, 2017;Wu et al, 2021). These proteins increased in abundance in BESC-22 within 24 hpi and decreased in abundance in BESC-801 across all timepoints (Figure 5).…”

Section: Systemic Acquired Resistance and Hypersensitive Response Pro...mentioning

confidence: 92%

“…Arabidopsis are induced by PTI and may act as membrane scaffolds for other defense-response proteins (Lv et al, 2017). HRLI proteins mediate cell death and are involved with SA defense signaling (Wu et al, 2021). As such, it is likely that the P. trichocarpa-S. musiva interaction begins with pathogen recognition that subsequently leads to PTI and SAR, which in turn primes adjacent plant cells for defense and HR.…”

Section: Systemic Acquired Resistance Is Elicited By Besc-22 During T...mentioning

confidence: 99%

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Proteomics reveals pathways linked to septoria canker resistance and susceptibility in Populus trichocarpa

et al. 2022

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A major threat to forest ecosystems and plantation forestry is the introduction of a non-native pathogen. Among non-domesticated populations with relatively high levels of genetic diversity, a measurable range of susceptibility to resistance can be expected. Identifying genetic determinants of resistant and susceptible individuals can inform the development of new strategies to engineer disease resistance. Here we describe pathogen-induced changes in the proteome of Populus trichocarpa stem tissue in response to Sphaerulia musiva (Septoria canker). This hemibiotrophic fungal pathogen causes stem canker disease in susceptible poplar genotypes. Proteomics analyses were performed on stem tissue harvested across 0-, 12-, 24- and 48-h post-inoculation with Septoria from three genotypes including one resistant (BESC-22) and two susceptible [BESC-801; Nisqually-1 (NQ-1)]. In total, 11,897 Populus proteins at FDR <0.01 were identified across all time points and genotypes. Analysis of protein abundances between genotypes revealed that the resistant poplar genotype (BESC-22) mounts a rapid and sustained defense response involving pattern recognition receptors, calcium signaling proteins, SAR inducers, transcriptional regulators, resistance proteins, and proteins involved with the hypersensitive response. One susceptible genotype (BESC-801) had a downregulated and delayed defense response whereas the second susceptible genotype (NQ-1) lacked a distinct pattern. Overall, the proteome-wide and protein-specific trends suggest that responses to the Septoria canker infection are genotype-specific for the naïve host, Populus trichocarpa.

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Section: Systemic Acquired Resistance and Hypersensitive Response Pro...mentioning

confidence: 92%

Section: Systemic Acquired Resistance Is Elicited By Besc-22 During T...mentioning

confidence: 99%

Proteomics reveals pathways linked to septoria canker resistance and susceptibility in Populus trichocarpa

et al. 2022

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“…In a recent report, we analysed the transcriptomics data of TuMV‐infected N. benthamiana (Wu et al., 2021). From the differentially expressed genes, we identified that the transcript level of NbeIF6 markedly increased in TuMV‐infected plants.…”

Section: Resultsmentioning

confidence: 99%

Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana

Chen,

Wang,

Chen

et al. 2024

Molecular Plant Pathology

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Viruses rely completely on host translational machinery to produce the proteins encoded by their genes. Controlling translation initiation is important for gaining translational advantage in conflicts between the host and virus. The eukaryotic translation initiation factor 4E (eIF4E) has been reported to be hijacked by potyviruses for virus multiplication. The role of translation regulation in defence and anti‐defence between plants and viruses is not well understood. We report that the transcript level of eIF6 was markedly increased in turnip mosaic virus (TuMV)‐infected Nicotiana benthamiana. TuMV infection was impaired by overexpression of N. benthamiana eIF6 (NbeIF6) either transiently expressed in leaves or stably expressed in transgenic plants. Polysome profile assays showed that overexpression of NbeIF6 caused the accumulation of 40S and 60S ribosomal subunits, the reduction of polysomes, and also compromised TuMV UTR‐mediated translation, indicating a defence role for upregulated NbeIF6 during TuMV infection. However, the polysome profile in TuMV‐infected leaves was not identical to that in leaves overexpressing NbeIF6. Further analysis showed that TuMV NIb protein, the RNA‐dependent RNA polymerase, interacted with NbeIF6 and interfered with its effect on the ribosomal subunits, suggesting that NIb might have a counterdefence role. The results propose a possible regulatory mechanism at the translation level during plant–virus interaction.

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“…Dof proteins are involved in various biological processes, including but not limited to endosperm development, plant defense, seed germination, gibberellin response and auxin response ( Yu et al., 2019 ). AP2/ERF proteins play vital roles in regulating plant growth and development and various abiotic stress response ( Wu et al., 2021 ). It has noticed that BnLORs were mainly regulated by these TFs, further supporting the prediction that BnLORs perform crucial functions in the regulation of abiotic stresses and hormone treatments.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

et al. 2023

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The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, there is a paucity of research investigating the role of the LOR gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 LOR genes in B. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 BnLORs could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 BnLOR members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that BnLORs involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of BnLOR family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate BnLOR gene expression under temperature, salinity and ABA stress, revealing that most BnLORs showed inducibility. This study enhanced our comprehension of the B. napus LOR gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.

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Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus

Cited by 6 publications

References 57 publications

Proteomics reveals pathways linked to septoria canker resistance and susceptibility in Populus trichocarpa

Proteomics reveals pathways linked to septoria canker resistance and susceptibility in Populus trichocarpa

Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana

Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

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