Genome-wide investigation and expression profiling of LOR gene family in rapeseed under salinity and ABA stress

Abstract

The Brassica napus (B. napus) LOR (Lurp-One-Related) gene family is a little-known gene family characterized by a conserved LOR domain in the proteins. Limited research in Arabidopsis showed that LOR family members played important roles in Hyaloperonospora parasitica (Hpa) defense. Nevertheless, there is a paucity of research investigating the role of the LOR gene family towards their responses to abiotic stresses and hormone treatments. This study encompassed a comprehensive survey of 56 LOR genes in B. napus, which is a prominent oilseed crop that holds substantial economic significance in China, Europe, and North America. Additionally, the study evaluated the expression profiles of these genes in response to salinity and ABA stress. Phylogenetic analysis showed that 56 BnLORs could be divided into 3 subgroups (8 clades) with uneven distribution on 19 chromosomes. 37 out of 56 BnLOR members have experienced segmental duplication and 5 of them have undergone tandem repeats events with strong evidence of purifying selection. Cis-regulatory elements (CREs) analysis indicated that BnLORs involved in process such as light response, hormone response, low temperature response, heat stress response, and dehydration response. The expression pattern of BnLOR family members revealed tissue specificity. RNA-Seq and qRT-PCR were used to validate BnLOR gene expression under temperature, salinity and ABA stress, revealing that most BnLORs showed inducibility. This study enhanced our comprehension of the B. napus LOR gene family and could provide valuable information for identifying and selecting genes for stress resistant breeding.

TFIIB-Related Protein BRP5/PTF2 Is Required for Both Male and Female Gametogenesis and for Grain Formation in Rice

“…Sequence analyses were conducted using the InterPro Program (http://www.ebi.ac.uk/interpro/ (accessed on 1 September 2023)). The evolutionary relationships between the TFIIB gene families were identified via comparing the protein sequences of TFIIB-associated proteins in Arabidopsis and rice using Clustal W with default parameters [27]. The neighbour-joining (NJ) method was used to construct the phylogenetic trees in MEGA 6.0.…”

Effect of methyl jasmonate and GA3 on canola (Brassica napus L.) growth, antioxidants activity, and nutrient concentration cultivated in salt-affected soils

“…Global challenges like climate change and urbanization highlight the need for plants to thrive in adverse conditions [ 1 , 2 ]. Factors such as marginal land use and unsustainable irrigation increase global salinity, threatening crop yields by disrupting plant physiological processes [ 3 – 5 ]. Salinity stress leads to elevated osmotic pressure and salt toxicity, impacting seed germination, growth, and reproductive behavior [ 6 , 7 ].…”