Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic
            <i>Ralstonia</i>
            Species

Georgoulis, Stratton J.; Shalvarjian, Katie E; Helmann, Tyler C.; Hamilton, Corri D.; Carlson, Hans K.; Deutschbauer, Adam M.; Lowe‐Power, Tiffany M.

doi:10.1128/msystems.01229-21

Cited by 10 publications

(5 citation statements)

References 66 publications

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“…vitians . A thorough comparison with other plant-pathogenic bacterial LPSs would be difficult because each bacterium has a specific set of genes involved in LPS biosynthesis ( 100 – 102 ). Yet, the accurate identification of the LPS biosynthesis genomic regions critical for in planta fitness of X. hortorum pv.…”

Section: Resultsmentioning

confidence: 99%

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

et al. 2022

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Xanthomonas hortorum was recently the subject of renewed interest, as several studies highlighted that its members were responsible for diseases in a wide range of plant species, including crops of agricultural relevance (e.g., tomato and carrot). Among X. hortorum variants, X. hortorum pv. vitians is a reemerging foliar hemibiotrophic phytopathogen responsible for severe outbreaks of bacterial leaf spot of lettuce all around the world.

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Section: Resultsmentioning

confidence: 99%

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

et al. 2022

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“…One limitation of our system is the time- and space-consuming process of collecting sufficient tomato xylem sap, which could be overcome by the development of defined media that mimics the sap biochemistry ( Khokhani et al, 2017 ; Gerlin et al, 2021 ). The system can be further optimized and integrated with molecular and imaging techniques, such as Tn-Seq ( Georgoulis et al, 2021 ), promoter probing ( Miller et al, 2000 ), and live-cell imaging ( De La Fuente et al, 2007 ; Kozgunova and Goshima, 2019 ) to unravel novel molecular mechanisms involved in biofilm formation and vessel blockage of other xylem-inhabiting pathogens, such as Xylella fastidiosa , Clavibacter michiganensis , Erwinia amylovora , and Erwinia tracheiphila , etc. It is estimated that 40%–80% prokaryotes on Earth exist in the form of biofilm ( Flemming and Wuertz, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Development of a tomato xylem-mimicking microfluidic system to study Ralstonia pseudosolanacearum biofilm formation

Chu,

Laxman,

Abdelhamed

et al. 2024

Front. Bioeng. Biotechnol.

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The bacterial wilt pathogen Ralstonia pseudosolanacearum (Rps) colonizes plant xylem vessels and blocks the flow of xylem sap by its biofilm (comprising of bacterial cells and extracellular material), resulting in devastating wilt disease across many economically important host plants including tomatoes. The technical challenges of imaging the xylem environment, along with the use of artificial cell culture plates and media in existing in vitro systems, limit the understanding of Rps biofilm formation and its infection dynamics. In this study, we designed and built a microfluidic system that mimicked the physical and chemical conditions of the tomato xylem vessels, and allowed us to dissect Rps responses to different xylem-like conditions. The system, incorporating functional surface coatings of carboxymethyl cellulose-dopamine, provided a bioactive environment that significantly enhanced Rps attachment and biofilm formation in the presence of tomato xylem sap. Using computational approaches, we confirmed that Rps experienced linear increasing drag forces in xylem-mimicking channels at higher flow rates. Consistently, attachment and biofilm assays conducted in our microfluidic system revealed that both seeding time and flow rates were critical for bacterial adhesion to surface and biofilm formation inside the channels. These findings provided insights into the Rps attachment and biofilm formation processes, contributing to a better understanding of plant-pathogen interactions during wilt disease development.

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“…However, recent quantitative metabolomics and physiological modeling show that healthy tomato xylem sap contains enough organic carbon and nitrogen to sustain Ralstonia ’s rapid growth, including mM levels of amino acids and 0.1-mM levels of glucose and sucrose ( 12 , 13 ). Xylem sap should thus be considered a rich, but incomplete, medium ( 37 ). In vitro experiments indicate that phyl.…”

Section: Discussionmentioning

confidence: 99%

Plant-Pathogenic Ralstonia Phylotypes Evolved Divergent Respiratory Strategies and Behaviors To Thrive in Xylem

Truchon

Dalsing

Khokhani

et al. 2023

mBio

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Plant-pathogenic Ralstonia spp. are a heterogeneous globally distributed group of bacteria that colonize plant xylem vessels. Ralstonia cells multiply rapidly in plants and obstruct water transport, causing fatal wilting and serious economic losses of many key food security crops. The virulence of these pathogens depends on their ability to grow to high cell densities in the low-oxygen xylem environment.

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Genome-Wide Identification of Tomato Xylem Sap Fitness Factors for Three Plant-Pathogenic Ralstonia Species

Cited by 10 publications

References 66 publications

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians

Development of a tomato xylem-mimicking microfluidic system to study Ralstonia pseudosolanacearum biofilm formation

Plant-Pathogenic Ralstonia Phylotypes Evolved Divergent Respiratory Strategies and Behaviors To Thrive in Xylem

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