Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis

The pivotal role of small non-coding RNAs in the regulation of seed development

“…miR159, miR166, miR168, miR171) and synchronized embryo development (e.g. miR156, miR167, miR397, miR398) (Zhang et al 2012a. Very recently, Li et al (2016) reported that overexpression of miR166 in somatic embryos of the gymnosperm Larix leptolepis resulted in a wider and flatter SAM, which is partially consistent with previous results from Arabidopsis (Williams et al 2005).…”

Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture

“…Previous studies have identified miRNAs associated with development and stress response in bread wheat by sequencing sRNA populations or through computational strategies (Jin et al, 2008; Kantar et al, 2012). Recent studies have indicated that miRNAs were associated with embryogenic callus formation in many plants (Luo et al, 2006; Wu et al, 2011; Zhang et al, 2012; Yang et al, 2013). Furthermore, we investigated the differential expression of miRNAs associated with embryogenic callus formation in IMEs and MEs and analyzed the function of their targets in bread wheat.…”

“…A unique set of miRNAs expressed or differentially expressed in embryogenic callus were identified in rice (Luo et al, 2006; Chen et al, 2014); miRNAs and their target genes were analyzed in cotton ( Gossypium hirsutum L.) revealing their regulation role during somatic embryogenesis (Yang et al, 2013), miRNA expression during somatic embryogenesis in citrus ( Citrus sinensis L.) shows that miR156, miR168, and miR171 as well as miR159, miR164, miR390, and miR397 are related to somatic embryo induction or formation (Wu et al, 2011). Stage-specific expression of miRNAs and their targets in larch ( Larix leptolepis Gordon) indicate that miR171a/b might exert function on proembryogenic masses, miR171c acts in the induction process; miR162 and miR168 exert their regulatory function during total somatic embryogenesis process (Zhang et al, 2012). However, the identification and possible modulation of miRNA in embryogenic callus formation in wheat has rarely been studied.…”

Forest tree genomics: 10 achievements from the past 10 years and future prospects

“…The number of transcribed genes appears to be 30 to 40 % larger than in any other tissue (Cairney and Pullman 2007;Yakovlev et al 2014) with high relevance to the gene network in A. thaliana (300-450 genes, Cairney and Pullman 2007;Zhang et al 2012a). Transcript profiles during zygotic embryogenesis are highly correlated between Pinus pinaster and A. thaliana, with only 3 % of the transcripts estimated to be gymnosperm-specific (de Vega-Bartol et al 2013).…”