Genome-wide identification of MATE, functional analysis and molecular dynamics of DcMATE21 involved in anthocyanin accumulation in Daucus carota

Saad, Kirti R.; Kumar, Gyanendra; Puthusseri, Bijesh; Srinivasa, Sudhanva M.; Giridhar, P.; Shetty, Nandini P.

doi:10.1016/j.phytochem.2023.113676

Cited by 4 publications

(2 citation statements)

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“…Previous research used molecular docking to show that the five amino acid residues of PrMATE1 may bind to PA (Morales‐Quintana et al, 2019). Moreover, molecular dynamics simulations have revealed that 10 key amino acids of DcMATE21 bind to anthocyanins (Saad et al, 2023). These results indicate that the amino acids of DTX/MATE binding to substrates may vary with the species.…”

Section: Discussionmentioning

confidence: 99%

“…Under the process of evolution, DTX/ MATE transporters have expanded considerably in plants, resulting in large gene families in different species (Upadhyay et al, 2019). To date, 45 DTX/MATE genes have been identified in rice (Wang et al, 2016), 70 in Medicago truncatula (Wang et al, 2017), 72 in cotton (Xu et al, 2019), 65 in persimmon (Zhu et al, 2019), and 45 in Daucus carota (Saad et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

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Preferential transport activity of DkDTX5/MATE5 affects the formation of different astringency in persimmon

Liu

Sun

et al. 2023

JIPB

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Proanthocyanidins (PAs) are specialized metabolites that influence persimmon fruit quality. Normal astringent (A)‐type and non‐astringent (NA)‐type mutants show significant variation in PA accumulation, but the influencing mechanism remains unclear. In this study, among the six identified DTXs/MATEs proteins associated with PA accumulation, we observed that allelic variation and preferential transport by DkDTX5/MATE5 induced variation in PA accumulation for A‐type and NA‐type fruit. The expression pattern of DkDTX5/MATE5 was correlated with PA accumulation in NA‐type fruit. Upregulation and downregulation of DkDTX5/MATE5 promoted and inhibited PA accumulation, respectively, in the NA‐type fruit. Interestingly, transporter assays of Xenopus laevis oocytes indicated that DkDTX5/MATE5 preferentially transported the PA precursors catechin, epicatechin, and epicatechin gallate, resulting in their increased ratios relative to the total PAs, which was the main source of variation in PA accumulation between the A‐type and NA‐type. The allele lacking Ser‐84 in DkDTX5/MATE5 was identified as a dominantly expressed gene in the A‐type and lost its transport function. Site‐directed mutagenesis revealed that DkDTX5/MATE5 binds to PA precursors via Ser‐84. These findings clarify the association between the transporter function of DkDTX5/MATE5 and PA variation, and can contribute to the breeding of new cultivars with improved fruit quality.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%