Genome-wide identification of MAPKKK genes and their responses to phytoplasma infection in Chinese jujube (Ziziphus jujuba Mill.)

Abstract

Background: Mitogen-activated protein kinase (MAPK) cascades play vital roles in signal transduction in response to a wide range of biotic and abiotic stresses. In a previous study, we identified ten ZjMAPKs and five ZjMAPKKs in the Chinese jujube genome. We found that some members of ZjMAPKs and ZjMAPKKs may play key roles in the plant's response to phytoplasma infection. However, how these ZjMAPKKs are modulated by ZjMAPKKKs during the response process has not been elucidated. Little information is available regarding MAPKKKs in Chinese jujube.Results: A total of 56 ZjMAPKKKs were identified in the jujube genome. All of these kinases contain the key S-TKc (serine/threonine protein kinase) domain, which is distributed among all 12 chromosomes. Phylogenetic analyses show that these ZjMAPKKKs can be classified into two subfamilies. Specifically, 41 ZjMAPKKKs belong to the Raf subfamily, and 15 belong to the MEKK subfamily. In addition, the ZjMAPKKKs in each subfamily share the same conserved motifs and gene structures. Only one pair of ZjMAPKKKs (15/16, on chromosome 5) was found to be tandemly duplicated. Using qPCR, the expression profiles of these MAPKKKs were investigated in response to infection with phytoplasma. In the three main infected tissues (witches' broom leaves, phyllody leaves, and apparently normal leaves), ZjMAPKKK26 and − 45 were significantly upregulated, and ZjMAPKKK3, − 43 and − 50 were significantly downregulated. ZjMAPKKK4, − 10, − 25 and − 44 were significantly and highly induced in sterile cultivated tissues infected by phytoplasma, while ZjMAPKKK6, − 7, − 17, − 18, − 30, − 34, − 35, − 37, − 40, − 41, − 43, − 46, − 52 and − 53 were significantly downregulated.Conclusions: For the first time, we present an identification and classification analysis of ZjMAPKKKs. Some ZjMAPKKK genes may play key roles in the response to phytoplasma infection. This study provides an initial understanding of the mechanisms through which ZjMAPKKKs are involved in the response of Chinese jujube to phytoplasma infection.

Comprehensive analysis of MAPK gene family in Populus trichocarpa and physiological characterization of PtMAPK3‐1 in response to MeJA induction

“…With the identification of MAPK cascade gene families in different plant species, we found that the gene number of MAPK family, MAPKK family and MAPKKK family differed among species. There were 20 MAPK genes in A. thaliana (Colcombet & Hirt, 2008; Ichimura et al, 2002; Jonak et al, 2002), 19 in P. trichocarpa , 14 in V. vinifera (Cakir & Kilickaya, 2015; Wang et al, 2014a), 14 in C. sativus (Wang et al, 2015), 13 in E. ulmoides , 12 in J. curcas (Wang et al, 2018), and 10 in Z. jujuba (Liu et al, 2017; Liu et al, 2020). The MAPKK genes were 10, 9, 5, 6, 5, 5, 5, 5 and the MAPKKK genes were 80, 104, 62, 59, 57, 65, 56, respectively in the above‐mentioned species.…”

Emerging Roles of Mitogen-Activated Protein Kinase Signaling Pathways in the Regulation of Fruit Ripening and Postharvest Quality

“…For example, ZjMKK5 was specifically expressed in flower buds, whereas ZjMKK2 was largely expressed in fruits. A subsequent study identified a total of 56 ZjMAPKKKs, but unfortunately, their expression profile in fruit development was not reported [44]. In grapevines, Wang et al (2014) [45] identified 45 VviMAPKKK genes in the grapevine genome.…”

The Crosstalk of the Salicylic Acid and Jasmonic Acid Signaling Pathways Contributed to Different Resistance to Phytoplasma Infection Between the Two Genotypes in Chinese Jujube

“…The scions grafted on healthy rootstocks were analyzed as a negative control and named "PZ_H" and "T13_H, " H meaning healthy. All experimental trees were cultivated under natural environmental conditions (Liu et al, 2020b). After grafting to the first time of sample collection, the scions began sprouting, spreading leaves, and grew up.…”