Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

Song, Lin; Li, Chao; Xie, Yangjie; Liu, Shikai; Zhang, Jiaren; Yao, Jun; Jiang, Chen; Li, Yun; Liu, Zhanjiang

doi:10.1016/j.fsi.2015.12.009

Cited by 53 publications

(55 citation statements)

References 73 publications

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“…Marcos‐López et al () reported upregulation of hsp70 in gills from fish infected with P. perurans at 21 dpe at 10.5°C to 11.5°C, and an average gross gill score of 3.3, in contrast to findings in this study. Similarly, hsp70 expression was found elevated in fish following infection with the parasite Enteromyxum leei (Sitjà‐Bobadilla, ), and in different viral and bacterial infections (Ackerman et al, ; Song et al, ). Elevated hsp70 has been suggested previously to be involved indirectly in cell proliferation (Marcos‐López et al, ), and, therefore, it could be speculated that apparent downregulation of the hsp70 gene in P. perurans ‐infected salmon at 10°C may have resulted in less severe pathology compared to infection at 15°C.…”

Section: Discussionmentioning

confidence: 98%

“…Marcos-López et al (2017) reported upregulation of hsp70 in gills from fish infected with P. perurans at 21 dpe at 10.5°C to 11.5°C, and an average gross gill score of 3.3, in contrast to findings in this study. Similarly, hsp70 expression was found elevated in fish following infection with the parasite Enteromyxum leei (Sitjà-Bobadilla, 2008), and in different viral and bacterial infections (Ackerman et al, 2000;Song et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Effects of temperature on amoebic gill disease development: Does it play a role?

Benedicenti

Pottinger

Collins

et al. 2019

Journal of Fish Diseases

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A relationship between increasing water temperature and amoebic gill disease (AGD) prevalence in Atlantic salmon (Salmo salar) has been noted at fish farms in numerous countries. In Scotland (UK), temperatures above 12°C are considered to be an important risk factor for AGD outbreaks. Thus, the purpose of this study was to test for the presence of an association between temperature and variation in the severity of AGD in Atlantic salmon at 10 and 15°C. The results showed an association between temperature and variation in AGD severity in salmon from analysis of histopathology and Paramoeba perurans load, reflecting an earlier and stronger infection post‐amoebae exposure at the higher temperature. While no significant difference between the two temperature treatment groups was found in plasma cortisol levels, both glucose and lactate levels increased when gill pathology was evident at both temperatures. Expression analysis of immune‐ and stress‐related genes showed more modulation in gills than in head kidney, revealing an organ‐specific response and an interplay between temperature and infection. In conclusion, temperature may not only affect the host response, but perhaps also favour higher attachment/growth capacity of the amoebae as seen with the earlier and stronger P. perurans infection at 15°C.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Effects of temperature on amoebic gill disease development: Does it play a role?

Benedicenti

Pottinger

Collins

et al. 2019

Journal of Fish Diseases

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“…In addition, a similar number of Hsp70 family genes were found between P. sinensis and other species, suggesting the evolutionary conservation of Hsp70 among different species (Song et al, 2016;Wang et al, 2019). In addition, a similar number of Hsp70 family genes were found between P. sinensis and other species, suggesting the evolutionary conservation of Hsp70 among different species (Song et al, 2016;Wang et al, 2019).…”

Section: Conservation Of Pshsp70/110 Family Genes In Evolutionmentioning

confidence: 67%

Genomewide identification and analysis of heat‐shock proteins 70/110 to reveal their potential functions in Chinese soft‐shelled turtle Pelodiscus sinensis

Liu

Han

Liu

et al. 2019

Ecology and Evolution

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Heat‐shock proteins 70/110 (Hsp70/110) are vital molecular chaperones and stress proteins whose expression and production are generally induced by extreme temperatures or external stresses. The Hsp70/110 family is largely conserved in diverse animals. Although many reports have studied and elaborated on the characteristics of Hsp70/110 in various species, the systematic identification and analysis of Hsp70/110 are still poor in turtles. In this study, a genomewide search was performed, and 18 candidate PsHSP70/110 family genes were identified in Chinese soft‐shelled turtle, Pelodiscus sinensis. These PsHSP70/110 proteins contained the conserved “heat shock protein 70” domain. Phylogenetic analysis of PsHSP70/110 and their homologs revealed evolutionary conservation of Hsp70/110 across different species. Tissue‐specific expression analysis showed that these PsHSP70/110 genes were differentially expressed in different tissues of P. sinensis. Furthermore, to examine the putative biological functions of PsHSP70/110, the dynamic expression of PsHSP70/110 genes was analyzed in the testis of P. sinensis during seasonal spermatogenesis following germ cell apoptosis. Notably, genes such as PsHSPA1B‐L, PsHSPA2, and PsHSPA8 were significantly upregulated in P. sinensis testes along with a seasonal decrease in apoptosis. Protein interaction prediction revealed that PsHSPA1B‐L, PsHSPA2, and PsHSPA8 may interact with each other and participate in the MAPK signaling pathway. Moreover, immunohistochemical analysis showed that PsHSPA1B‐L, PsHSPA2, and PsHSPA8 protein expression was associated with seasonal temperature variation. The expression profiling and interaction relationships of the PsHSPA1B‐L, PsHSPA2, and PsHSPA8 proteins implied their potential roles in inhibiting the apoptosis of germ cells in P. sinensis. These results provide insights into PsHSP70/110 functions and will serve as a rich resource for further investigation of HSP70/110 family genes in P. sinensis and other turtles.

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“…In catfish, families of HSP40, HSP60, HSP70, HSP90 and sHSP were characterized, and their expression profiles following E. ictaluri and F. columnare bacterial infection were determined. Pathogen-specific, tissue-specific and time-specific patterns were found of these heat shock genes after infection with the two diseases (Song et al, 2014, 2016; Xie et al, 2015). Most of the HSP genes were induced at the earliest stages of disease infections, while more and more HSP genes became down-regulated with the progression of the diseases.…”

Section: Part Iv: Heat Stressmentioning

confidence: 96%

“…Many gene families were induced after E. ictaluri infection, including heat shock proteins (Song et al, 2014, 2016; Xie et al, 2015), interlekin 17 (Wang X. et al, 2014), l-rhamnose-binding lectins (Thongda et al, 2014), nitric oxide synthase genes (Yao et al, 2014), claudins (Sun et al, 2015), peptidoglycan recognition proteins (Sun et al, 2014), Rab GTPases (Wang R. et al, 2014), Rho GTPase (Tan et al, 2017), suppressors of cytokine signaling genes (Yao et al, 2015), cytochrome P450 genes (Zhang et al, 2014), serpins (Li et al, 2015), bcl-2 genes (Yuan et al, 2016), cathepsins (Geng et al, 2015; Wang et al, 2015; Dong et al, 2016), tumor suppressor genes (Mu et al, 2015), complement regulatory protein genes (Jiang et al, 2015), septins (Fu et al, 2016), chemokines (Fu et al, 2017a,b,c), galectins (Zhou et al, 2016), apolipoproteins (Yang et al, 2017), receptor tyrosine kinases (Tian et al, 2015), NF-kB related genes (Wang et al, 2017c), phosphoinositide-3-kinases (Li et al, 2016), NCK and ABI adaptor genes (Zhou et al, 2017a), JAK and STAT genes (Jin et al, 2018). These studies indicated that most of the immune related genes were involved in the E. ictaluri disease responses.…”

Section: Part I: Enteric Septicemia Of Catfish (Esc)mentioning

confidence: 99%

A Review of Molecular Responses of Catfish to Bacterial Diseases and Abiotic Stresses

et al. 2018

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Catfish is one of the major aquaculture species in the United States. However, the catfish industry is threatened by several bacterial diseases such as enteric septicemia of catfish (ESC), columnaris disease and Aeromonas disease, as well as by abiotic stresses such as high temperature and low oxygen. Research has been conducted for several decades to understand the host responses to these diseases and abiotic stresses. With the development of sequencing technologies, and the application of genome-wide association studies in aquaculture species, significant progress has been made. This review article summarizes recent progress in understanding the molecular responses of catfish after bacterial infection and stress challenges, and in understanding of genomic and genetic basis for disease resistance and stress tolerance.

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Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

Cited by 53 publications

References 73 publications

Effects of temperature on amoebic gill disease development: Does it play a role?

Effects of temperature on amoebic gill disease development: Does it play a role?

Genomewide identification and analysis of heat‐shock proteins 70/110 to reveal their potential functions in Chinese soft‐shelled turtle Pelodiscus sinensis

A Review of Molecular Responses of Catfish to Bacterial Diseases and Abiotic Stresses

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