A Review of Molecular Responses of Catfish to Bacterial Diseases and Abiotic Stresses

Zhou, Tao; Yuan, Zihao; Tan, Suxu; Jin, Yulin; Yang, Yujia; Shi, Huitong; Wang, Wenwen; Niu, Donghong; Gao, Lei; Jiang, Weixing; Gao, Dongya; Liu, Zhanjian John

doi:10.3389/fphys.2018.01113

Cited by 39 publications

(37 citation statements)

References 196 publications

(292 reference statements)

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“…To insert the donor template carrying the cathelicidin gene, we used the small guide RNA (sgRNA) ( Table 1) that directed Cas9 nuclease to that specific location in a noncoding region of chromosome 1. The designed sgRNA was co-injected with Cas9 along with each donor DNA constructs in different concentrations (10,20, and 40 ng/μL). The microinjection solution mentioned above was injected into one-cell stage embryos as described by Khalil et al 2 using a microinjection system from Applied Scientific Instrumentation (Eugene, OR).…”

Section: Microinjection and Hatching Of Embryosmentioning

confidence: 99%

“…Channel catfish (Ictalurus punctatus) is an important food fish in the United States that has been impacted by diseases, especially in commercial aquaculture. Efforts have been geared towards increasing disease resistance in this species as 40% of catfish production was lost to disease at the beginning of the current decade 19 and continues to be problematic 20 . This is the case for aquaculture in general 19,21 .…”

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confidence: 99%

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CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

Simora

Xing

Bangs

et al. 2020

Sci Rep

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CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model organisms. Here, we succeeded in integrating with high efficiency an exogenous alligator cathelicidin gene into a targeted non-coding region of channel catfish (Ictalurus punctatus) chromosome 1 using two different donor templates (synthesized linear dsDNA and cloned plasmid DNA constructs). We also tested two different promoters for driving the gene, zebrafish ubiquitin promoter and common carp β-actin promoter, harboring a 250-bp homologous region flanking both sides of the genomic target locus. Integration rates were found higher in dead fry than in live fingerlings, indicating either off-target effects or pleiotropic effects. Furthermore, low levels of mosaicism were detected in the tissues of P1 individuals harboring the transgene, and high transgene expression was observed in the blood of some P1 fish. This can be an indication of the localization of cathelicidin in neutrophils and macrophage granules as also observed in most antimicrobial peptides. This study marks the first use of CRISPR/Cas9 HDR for gene integration in channel catfish and may contribute to the generation of a more efficient system for precise gene integration in catfish and other aquaculture species, and the development of gene-edited, disease-resistant fish.

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Section: Microinjection and Hatching Of Embryosmentioning

confidence: 99%

mentioning

confidence: 99%

CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

Simora

Xing

Bangs

et al. 2020

Sci Rep

Self Cite

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“…In the US, hybrid catfish are being raised more often, due to superior performance traits including faster growth and increased disease resistance. Reports suggest the hybrid catfish is more resistant to virulent A. hydrophila than the channel catfish [99,121]. Hybrid catfish with an average weight of 3.79 g were immersed in vaccine for 1 h at 32.8 • C. The vaccine dose was 1.67 × 10 7 CFU mL −1 .…”

Section: Motile Aeromonas Septicemia (Mas)mentioning

confidence: 99%

Review on Immersion Vaccines for Fish: An Update 2019

Bøgwald

Dalmo

2019

Microorganisms

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Immersion vaccines are used for a variety of aquacultured fish to protect against infectious diseases caused by bacteria and viruses. During immersion vaccination the antigens are taken up by the skin, gills or gut and processed by the immune system, where the resulting response may lead to protection. The lack of classical secondary responses following repeated immersion vaccination may partly be explained by the limited uptake of antigens by immersion compared to injection. Administration of vaccines depends on the size of the fish. In most cases, immersion vaccination is inferior to injection vaccination with regard to achieved protection. However, injection is problematic in small fish, and fry as small as 0.5 gram may be immersion vaccinated when they are considered adaptively immunocompetent. Inactivated vaccines are, in many cases, weakly immunogenic, resulting in low protection after immersion vaccination. Therefore, during recent years, several studies have focused on different ways to augment the efficacy of these vaccines. Examples are booster vaccination, administration of immunostimulants/adjuvants, pretreatment with low frequency ultrasound, use of live attenuated and DNA vaccines, preincubation in hyperosmotic solutions, percutaneous application of a multiple puncture instrument and application of more suitable inactivation chemicals. Electrostatic coating with positively charged chitosan to obtain mucoadhesive vaccines and a more efficient delivery of inactivated vaccines has also been successful.

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“…However, the availability of land and water resources for aquaculture is currently limited, while susceptibility to new diseases is increasing ( Ayyam et al, 2019 ; Lebel et al, 2019 ). This has facilitated continuous improvements in breeding programs by cross-breeding between different species to generate new hybrid varieties that can tolerate high stocking densities ( Dunham and Masser, 2012 ; Zhou et al, 2018 ). Clariid catfish ( Clarias spp.)…”

Section: Introductionmentioning

confidence: 99%

An Investigation of ZZ/ZW and XX/XY Sex Determination Systems in North African Catfish (Clarias gariepinus, Burchell, 1822)

et al. 2021

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An investigation of sex-specific loci may provide important insights into fish sex determination strategies. This may be useful for biotechnological purposes, for example, to produce all-male or all-female fish for commercial breeding. The North African catfish species, Clarias gariepinus, has been widely adopted for aquaculture because its superior growth and disease resistance render the species suitable for hybridization with other catfish to improve the productivity and quality of fish meat. This species has either a ZZ/ZW or XX/XY sex determination system. Here, we investigate and characterize these systems using high-throughput genome complexity reduction sequencing as Diversity Arrays Technology. This approach was effective in identifying moderately sex-linked loci with both single-nucleotide polymorphisms (SNPs) and restriction fragment presence/absence (PA) markers in 30 perfectly sexed individuals of C. gariepinus. However, SNPs based markers were not found in this study. In total, 41 loci met the criteria for being moderately male-linked (with male vs. female ratios 80:20 and 70:30), while 25 loci were found to be moderately linked to female sex. No strictly male- or female-linked loci were detected. Seven moderately male-linked loci were partially homologous to some classes of transposable elements and three moderately male-linked loci were partially homologous to functional genes. Our data showed that the male heterogametic XX/XY sex determination system should co-exist with the ZZ/ZW system in C. gariepinus. Our finding of the co-existence of XX/XY and ZZ/ZW systems can be applied to benefit commercial breeding of this species in Thailand. This approach using moderately sex-linked loci provides a solid baseline for revealing sex determination mechanisms and identify potential sex determination regions in catfish, allowing further investigation of genetic improvements in breeding programs.

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A Review of Molecular Responses of Catfish to Bacterial Diseases and Abiotic Stresses

Cited by 39 publications

References 196 publications

CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

CRISPR/Cas9-mediated knock-in of alligator cathelicidin gene in a non-coding region of channel catfish genome

Review on Immersion Vaccines for Fish: An Update 2019

An Investigation of ZZ/ZW and XX/XY Sex Determination Systems in North African Catfish (Clarias gariepinus, Burchell, 1822)

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