Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton

Huang, Hui; He, Ying; Cui, Aihua; Sun, Liangqing; Han, Mingge; Wang, Jing; Rui, Cun; Lei, Yuqian; Liu, Xiaoyu; Xu, Nan; Zhang, Hong; Zhang, Yuexin; Fan, Yapeng; Feng, Xixian; Ni, Kesong; Jiang, Jie; Zhang, Xingping; Chen, Chao; Wang, Shuai; Chen, Xiugui; Lu, Xuke; Wang, Delong; Wang, Junjuan; Yin, Zujun; Qaraevna, Bobokhonova Zebinisso; Guo, Lixue; Zhao, Lingxia; Ye, Wuwei

doi:10.3389/fgene.2022.965058

Cited by 8 publications

(9 citation statements)

References 61 publications

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“…In tomatoes, the upregulated expression of GAD1 can increase the contents of GABA and glutamate to alleviate salt stress damage [ 20 ]. In cotton, GAD6 plays an important role in responding to Cd 2+ stress [ 26 ].…”

Section: Synthesis and Metabolism Of Gabamentioning

confidence: 99%

Role of Gamma-Aminobutyric Acid in Plant Defense Response

et al. 2023

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Gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that acts as a defense substance and a signaling molecule in various physiological processes, and which helps plants respond to biotic and abiotic stresses. This review focuses on the role of GABA’s synthetic and metabolic pathways in regulating primary plant metabolism, redistributing carbon and nitrogen resources, reducing the accumulation of reactive oxygen species, and improving plants’ tolerance of oxidative stress. This review also highlights the way in which GABA maintains intracellular pH homeostasis by acting as a buffer and activating H+-ATPase. In addition, calcium signals participate in the accumulation process of GABA under stress. Moreover, GABA also transmits calcium signals through receptors to trigger downstream signaling cascades. In conclusion, understanding the role of GABA in this defense response provides a theoretical basis for applying GABA in agriculture and forestry and feasible coping strategies for plants in complex and changeable environments.

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Section: Synthesis and Metabolism Of Gabamentioning

confidence: 99%

Role of Gamma-Aminobutyric Acid in Plant Defense Response

et al. 2023

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“…To date, GAD has been reported in many crops that respond to various stresses. For instance, the cotton GhGAD6 responds to cadmium stress and increases the GABA content to relieve cadmium stress-induced oxidative damage [10]. Moreover, the activation of glutamate decarboxylase (GAD) requires the involvement of Ca 2+ .…”

Section: Introductionmentioning

confidence: 99%

The γ-Aminobutyric Acid (GABA) Synthesis Gene Regulates the Resistance to Water Core-Induced Hypoxia Stress for Pear Fruits

Liu

et al. 2023

Agronomy

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Watercore is a physiological disorder which often occurs in Rosaceae fruits, and it causes hypoxia stress, promoting fruit decay. γ-aminobutyric acid (GABA) was reported as being involved in different abiotic stresses, and glutamate decarboxylase (GAD) is the key enzyme of GABA synthesis in plants. Our previous transcriptome analysis found that PpGAD2 was significantly induced in watercore fruit; however, the mechanism through which PpGAD2 regulates watercore-induced hypoxia stress resistance in pears is unclear. The present study found that the fruit pulp ethanol, malondialdehyde (MDA) and H2O2 content was significantly inhibited by exogenous GABA. The transcript abundance of PpGAD2 was significantly higher than that of other PpGADs in watercore fruit or healthy fruit. Tissue expression showed that the content of PpGAD2 in mature fruit was higher than in young fruit. Moreover, subcellular localization showed that PpGAD2 was located in the cytoplasm. Transient overexpression assays suggested that PpGAD2 had a role in GABA synthesis. Several CML (calmodulin–like) genes were also significantly increased in watercore fruit. Moreover, PpWRKY53 was significantly induced in watercore fruit, and the GUS activity assay showed that PpWRKY53 can significantly increase the activity of the PpGAD2 promoter. Taken together, these results demonstrate that PpGAD2 played an important role in GABA synthesis to increase plants’ resistance to hypoxia stress, and its activity may be affected by PpWRKY53 and several watercore-induced CML genes.

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“…Hydrogen peroxide (H 2 O 2 ) was detected through staining with diaminobenzidine (DAB) after reaction, and ROS accumulation under drought stress was measured in leaves of plants pYL156 and pYL156-GhLCYε-3 (DAB) [33] . The leaves are placed in 1 g/L DAB staining solution, allowed to stand for 12 h, and decolorized with absolute ethanol [34] . The staining of the leaves was observed and recorded, and the black particles on the leaves were ROS [34] .…”

Section: Methodsmentioning

confidence: 99%

“…The leaves are placed in 1 g/L DAB staining solution, allowed to stand for 12 h, and decolorized with absolute ethanol [34] . The staining of the leaves was observed and recorded, and the black particles on the leaves were ROS [34] .…”

Section: Methodsmentioning

confidence: 99%