Genome-wide identification, classification and expression analysis of the Hsf and Hsp70 gene families in maize

Jiang, Ling-Yu; Hu, Weixin; Qian, Yexiong; Ren, Qiaoyu; Zhang, Jing

doi:10.1016/j.gene.2020.145348

Cited by 36 publications

(36 citation statements)

References 45 publications

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“…However, soybean [42], cabbage [43], and tobacco [10] have 61, 52, and 61 HSP70 genes, respectively. It is worth mentioning that potato, maize, and Arabidopsis have almost similar number of HSP70 genes (18-22 genes) [21,44] to the pumpkin. The different gene numbers of the same family in different species would be because of the size of the genome [42] or evolution diversity [45].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Heat Shock Protein 70 (HSP70) Gene Family in Pumpkin (Cucurbita moschata) Rootstock under Drought Stress Suggested the Potential Role of these Chaperones in Stress Tolerance

Davoudi

Chen

Lou

2022

IJMS

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Heat shock protein 70s (HSP70s) are highly conserved proteins that are involved in stress responses. These chaperones play pivotal roles in protein folding, removing the extra amounts of oxidized proteins, preventing protein denaturation, and improving the antioxidant system activities. This conserved family has been characterized in several crops under drought stress conditions. However, there is no study on HSP70s in pumpkin (Cucurbita moschata). Therefore, we performed a comprehensive analysis of this gene family, including phylogenetic relationship, motif and gene structure analysis, gene duplication, collinearity, and promoter analysis. In this research, we found 21 HSP70s that were classified into five groups (from A to E). These genes were mostly localized in the cytoplasm, chloroplast, mitochondria, nucleus, and endoplasmic reticulum (ER). We could observe more similarity in closely linked subfamilies in terms of motifs, the number of introns/exons, and the corresponding cellular compartments. According to the collinearity analysis, gene duplication had occurred as a result of purifying selection. The results showed that the occurrence of gene duplication for all nine gene pairs was due to segmental duplication (SD). Synteny analysis revealed a closer relationship between pumpkin and cucumber than pumpkin and Arabidopsis. Promoter analysis showed the presence of various cis-regulatory elements in the up-stream region of the HSP70 genes, such as hormones and stress-responsive elements, indicating a potential role of this gene family in stress tolerance. We furtherly performed the gene expression analysis of the HSP70s in pumpkin under progressive drought stress. Pumpkin is widely used as a rootstock to improve stress tolerance, as well as fruit quality of cucumber scion. Since stress-responsive mobile molecules translocate through vascular tissue from roots to the whole plant body, we used the xylem of grafted materials to study the expression patterns of the HSP70 (potentially mobile) gene family. The results indicated that all CmoHSP70s had very low expression levels at 4 days after stress (DAS). However, the genes showed different expression patterns by progressing he drought period. For example, the expression of CmoHSP70-4 (in subgroup E) and CmoHSP70-14 (in subgroup C) sharply increased at 6 and 11 DAS, respectively. However, the expression of all genes belonging to subgroup A did not change significantly in response to drought stress. These findings indicated the diverse roles of this gene family under drought stress and provided valuable information for further investigation on the function of this gene family, especially under stressful conditions.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Heat Shock Protein 70 (HSP70) Gene Family in Pumpkin (Cucurbita moschata) Rootstock under Drought Stress Suggested the Potential Role of these Chaperones in Stress Tolerance

Davoudi

Chen

Lou

2022

IJMS

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“…HSFA1 stimulates transcription of ERF/AP2 and DREB2A ( Mizoi et al, 2012 ), HSFA2 acts as a heat-inducible trans-activator of different genes ( Chauhan et al, 2013 ), and HSFA3 regulates the expression of DREB2A and DREB2C ( Chen et al, 2010 ). ZmHsf-6 belongs to class A1, ZmHsf-1 , ZmHsf-4 , ZmHsf-5 , and ZmHsf-17 belong to subclass A2, ZmHsf-3 , ZmHsf-11 , and ZmHsf-25 belong to class B, all contributing key roles in heat tolerance in maize ( Table 1 ; Lin et al, 2011 ; Zhang et al, 2020b ; Jiang et al, 2021 ). The expression of ZmHsf-6 was localized in pollens, and its expression was upregulated under heat stress ( Jiang et al, 2021 ).…”

Section: Molecular Mechanisms Of Plant Responses To Heat Stressmentioning

confidence: 99%

“…ZmHsf-6 belongs to class A1, ZmHsf-1 , ZmHsf-4 , ZmHsf-5 , and ZmHsf-17 belong to subclass A2, ZmHsf-3 , ZmHsf-11 , and ZmHsf-25 belong to class B, all contributing key roles in heat tolerance in maize ( Table 1 ; Lin et al, 2011 ; Zhang et al, 2020b ; Jiang et al, 2021 ). The expression of ZmHsf-6 was localized in pollens, and its expression was upregulated under heat stress ( Jiang et al, 2021 ). Furthermore, Hsp70-2 and Hsp70-4 are downstream targets of ZmHsf-6 and contribute significantly to abiotic stress response ( Li H.-C. et al, 2015 ).…”

Section: Molecular Mechanisms Of Plant Responses To Heat Stressmentioning

confidence: 99%

“…Recently, several gene families have been identified and subsequently characterized in maize involved in heat stress response, such as heat shock protein-70 and heat shock factor ( Casaretto et al, 2016 ; Jagtap et al, 2020 ; Jiang et al, 2021 ). Additionally, transcriptomic profiling of maize seedlings exposed to heat stress showed several differentially expressed genes employed in developing improved heat-tolerant maize varieties using robust genome editing techniques, such as RNAi and CRISPR/Cas9 ( El-Sappah et al, 2021 ; Razzaq et al, 2021 ; Singh et al, 2021 ).…”

Section: Approaches For Improving Thermotolerancementioning

confidence: 99%

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Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions

et al. 2022

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Graphical AbstractThis review summarized heat stress-mediated morphological and physiological changes in maize and elucidated the molecular mechanisms responsible for maize response to heat stress. Furthermore, plausible approaches to dissecting the regulatory network associated with heat stress response and improving maize adaptation to global warming have been discussed. This figure was made using BioRender.

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“…The Hsp70 family has been identi ed in many plants such as arabidopsis 18 genes (Lin et al 2001); potato 20 genes (Liu et al 2018); soybean 61 genes (Zhang et al 2015); pepper 21 genes (Guo et al 2016), maize 22 genes (Jiang et al 2020), and tobacco 61 genes (Song et al 2019). Many heat shock proteins, especially the Hsp70, were induced by heavy metal stress (Khan et al 2019).…”

Section: Introductionmentioning

confidence: 99%

The Hsp70 Gene family in Solanum Lycopersicum; Genome-wide Identification and Expression Analysis Under Heavy Metals Stresses

Sappah

Abbas

Elrys

et al. 2021

Preprint

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The Heat shock protein-70 (Hsp70) gene family is one of the protective mechanisms; however, it has not been widely studied in tomatoes. Therefore, the current study provides the first report genome-wide analysis of the Hsp70 gene family in tomato (Solanum lycopersicum L.) under five heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+) stresses. We identified 23 candidate genes of the Hsp70 gene family based on the PF00012 domain through bioinformatics studies, including gene structure, distribution, synteny, phylogenetic tree, protein-protein interactions, gene ontology, and previous RNA-seq data analysis followed by qRT-PCR analysis. Based on the phylogenetic analysis, the 23 candidate genes were classified into five subfamilies where the same subfamily contains similar SlHsp70 proteins. Many pairs of SlHsp70 gene duplications have appeared, consisting of tandem and segment duplication. In addition, analysis of previous RNA-seq besides the gene ontology gave us significant evidence about the vital roles of these genes during tomato development and growth. The SlHsp7s showed different responses, which were varied depend on different plant tissues and types of heavy metal. Some of the SlHsp70s were up-regulated after heavy metal exposure, such as Cd2+/SlHsp70-23 and Mn2+/ SlHsp70-8. Still, down-regulated others such as Fe2+/ SlHsp70-18. Finally, our gene expression analysis revealed the significant roles of the Hsp70s, especially, SlHsp70- 3, SlHsp70-8, SlHsp70-12, SlHsp70-19, and SlHsp70-23, with the different heavy metals treatments.

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Genome-wide identification, classification and expression analysis of the Hsf and Hsp70 gene families in maize

Cited by 36 publications

References 45 publications

Genome-Wide Identification and Expression Analysis of Heat Shock Protein 70 (HSP70) Gene Family in Pumpkin (Cucurbita moschata) Rootstock under Drought Stress Suggested the Potential Role of these Chaperones in Stress Tolerance

Genome-Wide Identification and Expression Analysis of Heat Shock Protein 70 (HSP70) Gene Family in Pumpkin (Cucurbita moschata) Rootstock under Drought Stress Suggested the Potential Role of these Chaperones in Stress Tolerance

Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions

The Hsp70 Gene family in Solanum Lycopersicum; Genome-wide Identification and Expression Analysis Under Heavy Metals Stresses

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