Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions

El-Sappah, Ahmed H.; Rather, Shabir A.; Wani, Shabir Hussain; Elrys, Ahmed S.; Bilal, Muhammad; Huang, Qiulan; Dar, Zahoor Ahmad; Elashtokhy, M.; Soaud, Nourhan; Koul, Monika; Mir, Reyazul Rouf; Ke, Yan; Li, Jia; El‐Tarabily, Khaled A.; Abbas, Manzar

doi:10.3389/fpls.2022.879366

Cited by 37 publications

(22 citation statements)

References 307 publications

(313 reference statements)

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“…Numerous studies have evidenced that gene expression analysis is a great strategy to identify positional and functional candidate genes associated with an organism’s response to heat stress [ 45 ]. Furthermore, the development of RNA-Seq technology has greatly improved the accuracy and comprehensiveness of gene expression analyses and accelerated the progress of heat stress-related research [ 84 , 85 ]. In the current study, RNA-Seq analysis was performed on three tissues (blood, liver, and adrenal glands) and four treatment groups (CT, H30, H60, and H120) and was not only able to identify the known mRNA isoforms annotated to known genes but also detected some novel transcripts associated with non-annotated genes.…”

Section: Resultsmentioning

confidence: 99%

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats

Dou

Sammad

Cánovas

et al. 2022

Biology

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The molecular mechanisms underlying heat stress tolerance in animals to high temperatures remain unclear. This study identified the differentially expressed mRNA isoforms which narrowed down the most reliable DEG markers and molecular pathways that underlie the mechanisms of thermoregulation. This experiment was performed on Sprague Dawley rats housed at 22 °C (control group; CT), and three acute heat-stressed groups housed at 42 °C for 30 min (H30), 60 min (H60), and 120 min (H120). Earlier, we demonstrated that acute heat stress increased the rectal temperature of rats, caused abnormal changes in the blood biochemical parameters, as well as induced dramatic changes in the expression levels of genes through epigenetics and post-transcriptional regulation. Transcriptomic analysis using RNA-Sequencing (RNA-Seq) data obtained previously from blood (CT and H120), liver (CT, H30, H60, and H120), and adrenal glands (CT, H30, H60, and H120) was performed. The differentially expressed mRNA isoforms (DEIs) were identified and annotated by the CLC Genomics Workbench. Biological process and metabolic pathway analyses were performed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 225, 5764, and 4988 DEIs in the blood, liver, and adrenal glands were observed. Furthermore, the number of novel differentially expressed transcript lengths with annotated genes and novel differentially expressed transcript with non-annotated genes were 136 and 8 in blood, 3549 and 120 in the liver, as well as 3078 and 220 in adrenal glands, respectively. About 35 genes were involved in the heat stress response, out of which, Dnaja1, LOC680121, Chordc1, AABR07011951.1, Hsp90aa1, Hspa1b, Cdkn1a, Hmox1, Bag3, and Dnaja4 were commonly identified in the liver and adrenal glands, suggesting that these genes may regulate heat stress response through interactions between the liver and adrenal glands. In conclusion, this study would enhance our understanding of the complex underlying mechanisms of acute heat stress, and the identified mRNA isoforms and genes can be used as potential candidates for thermotolerance selection in mammals.

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Section: Resultsmentioning

confidence: 99%

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats

Dou

Sammad

Cánovas

et al. 2022

Biology

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“…Using this system, we showed that chloroplast degradation caused by heat stress likely enhances chloroplast-to-mitochondria gene transfer. cpDNA leaked from the degraded chloroplasts may be incorporated into mitochondria via the active import system through VDAC channels [28] or via increased membrane permeability caused by heat shock [29], and then integrated into mtDNA by homologous recombination, which is active in land plants [3]. As heat stress affects the integrity of all membranes, including nuclear, chloroplast, and mitochondrial membranes [29], gene ux among these organelles may promote adaptation to environmental stresses.…”

Section: Discussionmentioning

confidence: 99%

Ongoing gene transfer from chloroplasts to mitochondria in tobacco

Odahara

Mori

Numata

2023

Preprint

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Angiosperm mitochondrial genomes have highly complex and diverse structures that are partly due to frequent insertions of nuclear and chloroplast DNA (cpDNA) into mitochondrial DNA (mtDNA). This suggests the existence of mechanisms for gene transfer from chloroplasts to mitochondria, but these have yet to be discovered. In this study, we aimed to detect chloroplast-to-mitochondrion gene transfer by analyzing the translocation of a marker gene, sul, encoding a bacterial dihydropteroate synthase that confers sulfonamide resistance in tobacco (Nicotiana tabacum), to mtDNA. First, we created tobacco chloroplast transformants in which sul, surrounded on both sides by ~ 1 kb of mitochondrial homologous sequences that enable targeted integration into mtDNA, was introduced into the chloroplast genome. Heat shock enhanced sul expression in the transformants, suggesting that chloroplast degradation stimulates gene transfer from chloroplasts to mitochondria. Shoot regeneration using the heat-shocked chloroplast transformants under sulfadiazine selection resulted in several transformants showing moderately resistant to sulfadiazine. Deep sequencing analysis of the target mitochondrial locus detected sul in the SR plants with an integration efficiency of 0.0007–0.0036%, and we validated the results by ruling out two types of artifactual outcomes, PCR jumping and sul integration into nuclear mitochondrial DNA (NuMT). From these results, we propose that gene transfer from chloroplasts to mitochondria is ongoing in tobacco.

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“…Numerous studies have evidenced that the gene expression analysis is a great strategy to identify positional and functional candidate genes associated with an organism's response to heat stress [45]. Furthermore, the development of RNA-Seq technology had greatly improved the accuracy and comprehensiveness of gene expression analyses, and accelerated the progress of heat stress related research [84,85]. In the current study, RNA-Seq analysis was performed on 3 tissues (blood, liver, and adrenal glands) and 4 treatment groups (CT, H30, H60 and H120) and was not only able to identify the known mRNA isoforms annotated to known genes, but also detected some novel transcripts associated with non-annotated genes.…”

Section: Summary Of Differential Isoform Corresponding Genes That Wer...mentioning

confidence: 99%

Identification of Novel Mrna Isoforms Associated with Acute Heat Stress Response Using RNA-Sequencing Data in The Sprague-Dawley Rats

Dou¹,

Sammad²,

Cánovas³

et al. 2022

Preprint

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The molecular mechanisms underlying heat stress tolerance in animals to high temperatures remain unclear. This experiment was performed with Sprague-Dawley rats housed at 22 ℃ (control group; CT), and 42 ℃ for 30 min (H30), 60 min (H60), and 120 min (H120). Transcriptomic analysis using RNA-Sequencing (RNA-Seq) from blood (CT and H120), liver (CT, H30, H60, and H120), and adrenal glands (CT, H30, H60, and H120) was performed. The differentially expressed mRNA isoforms (DEIs) were identified and annotated by the CLC Genomics Workbench. Biological process and metabolic pathway analyses were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 225, 5,764, and 4,988 DEIs in the blood, liver, and adrenal glands were observed. Furthermore, the number of novel differentially expressed transcript lengths with annotated genes and the novel differentially expressed transcript with non-annotated genes were 136 and 8 in blood, 3,549 and 120 in the liver, as well as 3,078 and 220 in adrenal glands, respectively. A total of 35 genes were involved in the heat stress response, out of which Dnaja1, LOC680121, Chordc1, AABR07011951.1, Hsp90aa1, Hspa1b, Cdkn1a, Hmox1, Bag3 and Dnaja4 were commonly identified in the liver and adrenal glands, suggesting that these genes may regulate heat stress response through interactions between liver and adrenal glands. The results suggest that the identified mRNA isoforms could be considered as potential candidates for selecting mammals to improve thermotolerance.

show abstract

Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions

Cited by 37 publications

References 307 publications

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats

Identification of Novel mRNA Isoforms Associated with Acute Heat Stress Response Using RNA Sequencing Data in Sprague Dawley Rats

Ongoing gene transfer from chloroplasts to mitochondria in tobacco

Identification of Novel Mrna Isoforms Associated with Acute Heat Stress Response Using RNA-Sequencing Data in The Sprague-Dawley Rats

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