Genome-wide identification, classification, and analysis of NADP-ME family members from 12 crucifer species

Peng, Tao; Guo, Weiling; Li, Biyuan; Wang, Wuhong; Yue, Zhichen; Lei, Juanli; Zhao, Yong; Zhong, Xinmin

doi:10.1007/s00438-016-1174-3

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…There are many cis-acting elements related to salt stress response on DsME1P and DsME2P such as ABRE. Meanwhile, ABRE was also found in the promoter of ME in other plants . Under salt stress, ABA can regulate downstream gene expression by binding with ABRE on the promoter in plants. , However, a unique MYB binding site (MBS) was identified on DsME2P, which was not present on DsME1P.…”

Section: Discussionmentioning

confidence: 99%

Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

Dai,

He,

Chen

et al. 2023

J. Agric. Food Chem.

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Triacylglycerols (TAG) from microalgae can be used as feedstocks for biofuel production to address fuel shortages. Most of the current research has focused on the enzymes involved in TAG biosynthesis. In this study, the effects of malic enzyme (ME), which provides precursor and reducing power for TAG biosynthesis, on biomass and lipid accumulation and its response to salt stress in Dunaliella salina were investigated. The overexpression of DsME1 and DsME2 improved the lipid production, which reached 0.243 and 0.253 g/L and were 30.5 and 36.3% higher than wild type, respectively. The transcript levels of DsME1 and DsME2 increased with increasing salt concentration (0, 1, 2, 3, and 4.5 mol/L NaCl), indicating that DsMEs participated in the salt stress response in D. salina. It was found that cis-acting elements associated with the salt stress response were present on the promoters of two DsMEs. The deletion of the MYB binding site (MBS) on the DsME2 promoter confirmed that MBS drives the expression of DsME2 to participate in osmotic regulation in D. salina. In conclusion, MEs are the critical enzymes that play pivotal roles in lipid accumulation and osmotic regulation.

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Section: Discussionmentioning

confidence: 99%

Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

Dai,

He,

Chen

et al. 2023

J. Agric. Food Chem.

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“…The encoding NADP-MEs share a high degree of identity with other plant NADP-MEs, and they have distinctive domains [ 45 ]. Furthermore, the four CaNADP-MEs identified in fruits were predicted to be located in the cytosol (CaNADP-ME2) and in the plastids (CaNADP-ME3 to CaNADP-ME3 5), which is analogous to other plant species [ 9 , 47 , 59 , 60 ]. Thus, the model plant Arabidopsis contains four NADP-ME genes in its genome, and three encode for cytosolic isozymes (NADP-ME1 to NADP-ME3), whereas NADP-ME2 is located in plastids [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO)

Taboada

González‐Gordo

Muñoz‐Vargas

et al. 2023

Plants

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NADPH is an indispensable cofactor in a wide range of physiological processes that is generated by a family of NADPH dehydrogenases, of which the NADP-dependent malic enzyme (NADP-ME) is a member. Pepper (Capsicum annuum L.) fruit is a horticultural product consumed worldwide that has great nutritional and economic relevance. Besides the phenotypical changes that pepper fruit undergoes during ripening, there are many associated modifications at transcriptomic, proteome, biochemical and metabolic levels. Nitric oxide (NO) is a recognized signal molecule with regulatory functions in diverse plant processes. To our knowledge, there is very scarce information about the number of genes encoding for NADP-ME in pepper plants and their expression during the ripening of sweet pepper fruit. Using a data mining approach to evaluate the pepper plant genome and fruit transcriptome (RNA-seq), five NADP-ME genes were identified, and four of them, namely CaNADP-ME2 to CaNADP-ME5, were expressed in fruit. The time course expression analysis of these genes during different fruit ripening stages, including green immature (G), breaking point (BP) and red ripe (R), showed that they were differentially modulated. Thus, while CaNADP-ME3 and CaNADP-ME5 were upregulated, CaNADP-ME2 and CaNADP-ME4 were downregulated. Exogenous NO treatment of fruit triggered the downregulation of CaNADP-ME4. We obtained a 50–75% ammonium–sulfate-enriched protein fraction containing CaNADP-ME enzyme activity, and this was assayed via non-denaturing polyacrylamide gel electrophoresis (PAGE). The results allow us to identify four isozymes designated from CaNADP-ME I to CaNADP-ME IV. Taken together, the data provide new pieces of information on the CaNADP-ME system with the identification of five CaNADP-ME genes and how the four genes expressed in pepper fruits are modulated during ripening and exogenous NO gas treatment.

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“…LOC_Os03g15050 encodes a transaldolase, which plays an important role in glycolysis and the pentose phosphate pathway (PPP) (Caillau and Paul Quick 2005 ). In addition, LOC_Os02g44550 (an NADP-dependent malic enzyme) and LOC_Os07g42440 (a glycolate oxidase) have important functions in photosynthesis (Cui et al 2016 ; Tao et al 2016 ). It was recently found that splicing factors participated in flower and embryo development in Arabidopsis (Xiong et al 2019a , b ), and seed development in maize (Fouquet et al 2011 ).…”

Section: Discussionmentioning

confidence: 99%

A Cyclophilin OsCYP20–2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing

Tang

Luo

et al. 2020

Rice

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Background Grain size is one of the key agronomic traits that impact grain yield. Several regulatory pathways had been reported to participate in grain size determination via cell expansion or proliferation in rice. However, little is known about cyclophilin and spliceosome participation in grain shape regulation. Results Here, we identified OsCYP20–2, a cyclophilin that influences spliceosome assembly to determine grain length. oscyp20–2 t1, a knock out mutant of OsCYP20–2 caused by T-DNA insertion, produced shorter grains with deficient cell elongation. Through yeast two-hybrid screening and pull-down assays, OsSYF2, a pre-mRNA splicing factor, was identified as an interacting protein of OsCYP20–2. The phenotypes of transgenic lines indicated that OsSYF2 positively regulates grain length via its influence on cell expansion. Transcriptomic analysis showed that OsSYF2 controls the expression and pre-mRNA alternative splicing of genes involved in sugar metabolism. In addition, these two genes have similar effects on panicle architecture. Conclusions Taken together, OsSYF2, an interacting protein of OsCYP20–2, controls grain length and panicle architecture by regulating the alternative splicing of pre-mRNA involved in cell elongation and sugar metabolism.

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Genome-wide identification, classification, and analysis of NADP-ME family members from 12 crucifer species

Cited by 10 publications

References 44 publications

Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO)

A Cyclophilin OsCYP20–2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing

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