2023
DOI: 10.1021/acs.jafc.3c04810
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Dual Roles of Two Malic Enzymes in Lipid Biosynthesis and Salt Stress Response in Dunaliella salina

Jv-Liang Dai,
Yu-Jing He,
Hao-Hong Chen
et al.

Abstract: Triacylglycerols (TAG) from microalgae can be used as feedstocks for biofuel production to address fuel shortages. Most of the current research has focused on the enzymes involved in TAG biosynthesis. In this study, the effects of malic enzyme (ME), which provides precursor and reducing power for TAG biosynthesis, on biomass and lipid accumulation and its response to salt stress in Dunaliella salina were investigated. The overexpression of DsME1 and DsME2 improved the lipid production, which reached 0.243 and … Show more

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Cited by 2 publications
(2 citation statements)
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“…pZBEL-GP was preserved in our laboratory and used for the construction of gene overexpression in Dunaliella. We inserted DbLyISO gene fragment to pRSFDuet-1 ( Nco I- EcoR I) to construct pRSFDuet-LyISO. Plasmid multiplication was performed using E.…”
Section: Methodsmentioning
confidence: 99%
“…pZBEL-GP was preserved in our laboratory and used for the construction of gene overexpression in Dunaliella. We inserted DbLyISO gene fragment to pRSFDuet-1 ( Nco I- EcoR I) to construct pRSFDuet-LyISO. Plasmid multiplication was performed using E.…”
Section: Methodsmentioning
confidence: 99%
“…To elevate DHA contents in Schizochytrium sp., CcME and MaELO3 were co-expressed; thus, DHA content was increased by 3.3-fold [137]. In Dunaliella salina, co-expression of DsME1 and DsME2 improved lipid production by up to 36.3% higher than the wild-type [138]. In Scenedesmus sp.…”
Section: Genetic Engineering Of Microalgae For Enhanced Lipid Productionmentioning
confidence: 99%