Genome-wide identification, characterization, and expression analysis of the TRAF gene family in Chinese tongue sole (Cynoglossus semilaevis)

Li, Ming; Wang, Na; Chen, Yadong; Xu, Xiwen; Xu, Wenteng; Wang, Lei; Chen, Songlin

doi:10.1016/j.fsi.2019.11.029

Cited by 20 publications

(18 citation statements)

References 56 publications

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“…To investigate the tissue expression profile of EcTRAF4 under normal physiological conditions, 10 different tissues from healthy grouper were analyzed with RT–qPCR. EcTRAF4 was expressed in all the examined tissues and predominantly expressed in the spleen, gill, skin, and stomach, similar to most fish TRAF genes [ 24 , 26 , 28 ]. To investigate the involvement of TRAFs in the innate immune responses of teleost fish, the expression profile of EcTRAF4 in GS cells after challenges with RGNNV was examined.…”

Section: Discussionmentioning

confidence: 77%

“…As an important family of signal transduction proteins, the TRAF proteins have been found in all animal species [ 24 ]. Although several TRAF family members in teleosts had been studied, and the results showed that they played important roles in the innate immune system [ 25 , 26 , 27 , 28 ], the function of TRAF4 in the orange-spotted grouper had not been established.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, fish TRAF genes were shown to be widely expressed in the examined tissues [ 24 ]. Most TRAF genes were highly expressed in the gills.…”

Section: Discussionmentioning

confidence: 99%

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Grouper TRAF4, a Novel, CP-Interacting Protein That Promotes Red-Spotted Grouper Nervous Necrosis Virus Replication

Sun

Zhang

et al. 2021

IJMS

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Tumor necrosis factor receptor-associated factors (TRAFs) play important roles in the biological processes of immune regulation, the inflammatory response, and apoptosis. TRAF4 belongs to the TRAF family and plays a major role in many biological processes. Compared with other TRAF proteins, the functions of TRAF4 in teleosts have been largely unknown. In the present study, the TRAF4 homologue (EcTRAF4) of the orange-spotted grouper was characterized. EcTRAF4 consisted of 1413 bp encoding a 471-amino-acid protein, and the predicted molecular mass was 54.27 kDa. EcTRAF4 shares 99.79% of its identity with TRAF4 of the giant grouper (E. lanceolatus). EcTRAF4 transcripts were ubiquitously and differentially expressed in all the examined tissues. EcTRAF4 expression in GS cells was significantly upregulated after stimulation with red-spotted grouper nervous necrosis virus (RGNNV). EcTRAF4 protein was distributed in the cytoplasm of GS cells. Overexpressed EcTRAF4 promoted RGNNV replication during viral infection in vitro. Yeast two-hybrid and coimmunoprecipitation assays showed that EcTRAF4 interacted with the coat protein (CP) of RGNNV. EcTRAF4 inhibited the activation of IFN3, IFN-stimulated response element (ISRE), and nuclear factor-κB (NF-κB). Overexpressed EcTRAF4 also reduced the expression of interferon (IFN)-related molecules and pro-inflammatory factors. Together, these results demonstrate that EcTRAF4 plays crucial roles in RGNNV infection.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

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Grouper TRAF4, a Novel, CP-Interacting Protein That Promotes Red-Spotted Grouper Nervous Necrosis Virus Replication

Sun

Zhang

et al. 2021

IJMS

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“…So far there are few studies on IRAKs from teleosts. Recently, some reports suggest that fish IRAK4 could suppress MyD88-dependent NF-kB activation (77,79). In this study, yellow catfish irak1, 3, and 4 mRNAs were distinctly induced after E. ictaluri infection and the three iraks genes had a death domain and a kinase domain as mammals.…”

Section: Expression Changes and Potential Roles Of Inflammation-relatmentioning

confidence: 55%

“…TRAF proteins consist of a unique and highly conserved MATH domain, but the C-terminus of TRAF7 is replaced by seven WD40 repeats (76). In teleosts, little is known about the traf gene family except that fish traf6 might be involved in the innate immune response (77). In the present study, yellow catfish traf1, 2, 5 and 7 were detected to have conserved protein structures, and traf1, 2 and 7 mRNAs were notably up-regulated after E. ictaluri infection.…”

Section: Expression Changes and Potential Roles Of Inflammation-relatmentioning

confidence: 99%

The Dynamic Immune Response of Yellow Catfish (Pelteobagrus fulvidraco) Infected With Edwardsiella ictaluri Presenting the Inflammation Process

Zhou

Zhang

et al. 2021

Front. Immunol.

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Edwardsiella ictaluri is a highly destructive pathogen in cultured yellow catfish, thus it was very necessary to study the immune response of yellow catfish against bacterial infection. In this study, RNA-Seq technology was used to study the immune response in two distinct tissues of yellow catfish at eight different time points (h) after E. ictaluri infection. The number of differentially expressed genes (DEGs) in the spleen and liver was low at 3 h and 6 h post-infection, respectively. Afterwards, the most number of DEGs in the spleen was detected at 72 h, while the number of DEGs in the liver maintained a high level from 24 h to 120 h. The GO and KEGG enrichment analyses of DEGs at different time points uncovered that cytokines were continuously transcribed at 6 h to 120 h; whereas the liver is the main organ that secretes the components of the complement system, and metabolic regulation was activated from 12 h to 120 h. Moreover, an overview of the inflammation response of yellow catfish was exhibited including pattern-recognition receptors, inflammatory cytokines, chemokines, complements, and inflammation-related signal pathways. The similar expression tendency of nine genes by qRT-PCR validated the accuracy of transcriptome analyses. The different transcriptomic profiles obtained from the spleen and liver will help to better understand the dynamic immune response of fish against bacterial infection, and will provide basic information for establishing effective measures to prevent and control diseases in fish.

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Paeonol increases the antioxidant and anti-inflammatory capacity of gibel carp (Carassius auratus gibelio) challenged with Aeromonas hydrophila

Chen

et al. 2022

Fish & Shellfish Immunology

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Genome-wide identification, characterization, and expression analysis of the TRAF gene family in Chinese tongue sole (Cynoglossus semilaevis)

Cited by 20 publications

References 56 publications

Grouper TRAF4, a Novel, CP-Interacting Protein That Promotes Red-Spotted Grouper Nervous Necrosis Virus Replication

Grouper TRAF4, a Novel, CP-Interacting Protein That Promotes Red-Spotted Grouper Nervous Necrosis Virus Replication

The Dynamic Immune Response of Yellow Catfish (Pelteobagrus fulvidraco) Infected With Edwardsiella ictaluri Presenting the Inflammation Process

Paeonol increases the antioxidant and anti-inflammatory capacity of gibel carp (Carassius auratus gibelio) challenged with Aeromonas hydrophila

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