Paeonol increases the antioxidant and anti-inflammatory capacity of gibel carp (Carassius auratus gibelio) challenged with Aeromonas hydrophila

Gu, Yipeng; Chen, Kai; Xi, Bingwen; Xie, Jun; Bing, Xuwen

doi:10.1016/j.fsi.2022.03.005

Cited by 7 publications

(3 citation statements)

References 65 publications

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“…Quantitative real-time PCR was performed on a Bio-Rad CFX realtime PCR detection system (USA) using ChamQ Universal SYBR qPCR Master Mix kit (Vazyme, China). All primers of virulence related genes (Table 1) were synthesized by Sangon Biotech (China), and recA was chosen as the reference housekeeping gene (Pang, 2015;Gu et al, 2022). The experiments were conducted in triplicate, and the result was calculated using 2 -△△Ct method.…”

Section: Rna Isolation and Gene Expression Analyzesmentioning

confidence: 99%

Effects of glycyrrhetinic acid β on growth and virulence of Aeromonas hydrophila

Chen

Qin²,

Pan³

et al. 2023

Front. Microbiol.

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Aeromonas hydrophila is a significant pathogen to freshwater farmed animals, and antibiotics are usually used to control the bacterial septicemia caused by A. hydrophila. Due to the severe situation of development and spread of antibiotic resistance, there are stricter restrictions on antibiotics used in aquaculture. To evaluate the feasibility of glycyrrhetinic acid β (GA) as an alternative therapy against bacterial infection, in this study, an A. hydrophila isolated from diseased fish is used to test the antibacterial, anti-virulence activity and therapeutic effect of GA in vitro and in vivo, respectively. Results showed that GA did not affect the growth of A. hydrophila in vitro, while it could down-regulate (p < 0.05) the mRNA expression of the hemolysis-related genes hly and aerA, and significantly inhibited (p < 0.05) hemolytic activity of A. hydrophila. In addition, in vivo test showed that oral administration of GA was ineffective in controlling acute infections caused by A. hydrophila. In conclusion, these findings suggested that GA was a potential anti-virulence candidate against A. hydrophila, but the application of GA for the prevention and treatment of A. hydrophila-related diseases was still a long way.

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Section: Rna Isolation and Gene Expression Analyzesmentioning

confidence: 99%

Effects of glycyrrhetinic acid β on growth and virulence of Aeromonas hydrophila

Chen

Qin²,

Pan³

et al. 2023

Front. Microbiol.

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Section: Introductionmentioning

confidence: 99%

“…As the opportunistic pathogens of fish, A. hydrophila and A. veronii caused the death of fish by inducing motile Aeromonad septicaemia (Lewbart, 2001). Numerous studies have shown that excessive inflammatory reaction was the main reason of high mortality of fish infected by A. hydrophila or A. veronii (Amin, 2019; Yg et al, 2022). In recent years, more and more natural medicines were isolated from plants for combating drug resistance strains of A. hydrophila and A. veronii because of the negative effect elicited by antibiotic resistance and the unavailable of injection of fishery vaccine (Dong et al, 2020; Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The oral protective efficacy of magnolol against Aeromonas hydrophila and A. veronii infection via enhancing anti‐inflammatory ability in goldfish (Carassius auratus)

Zhang,

Li,

Wang

et al. 2023

Journal of Fish Diseases

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Aeromonas hydrophila and A. veronii are widespread and important critical pathogenic bacteria in the aquaculture industry and cause severe economic damage. At present, magnolol has been proved to be a broad‐spectrum antibacterial activity, such as A. hydrophila, Staphylococcus aureus and Streptococcus mutans. In order to explore the cause of in vivo disease resistance of magnolol and promote its safe application in aquaculture, the pathological detection and changes in immune indicators of fish after feeding with magnolol were conducted in this paper. Results showed that the diets supplemented with magnolol (3 g magnolol/kg commercial feed) significantly increase the expression level of anti‐inflammatory cytokines (IL‐10, TGF‐β and IL‐4) in the liver of goldfish (p < .05). Additionally, the expression levels of proinflammatory cytokines (IL‐1β, IL‐8 and IFN‐γ) did not increase significantly. Subsequently, this study investigated the resistance of goldfish to A. hydrophila and A. veronii infection after feeding with magnolol. The results showed that the survival rates of treatment groups fed 3 g magnolol/kg commercial feed daily increased by 23.1% and 38.5% after 10 days post A. hydrophila and A. veronii (p = .0351) infection, respectively. Meanwhile, growth performance (body weight and length), major internal organs (liver, spleen, kidney and intestine) and the serum biochemistry indicators (ATL and AST) all exhibited no significant adverse effects after the goldfish fed with magnolol for 30 days. TP showed an increasing concentration in the treatment group (p < .05). Results of the mRNA expression of stress response indicated that the expression level of cyp1a and hsp70 was significantly down‐regulated after a 30‐day treatment (p < .05), and the two genes recovered to the similar level as the control group after a commercial feed diet. In brief, the diets supplemented with magnolol protected the host from the excessive immune response caused by A. hydrophila and A. veronii via enhancing its anti‐inflammatory capacity and had no adverse effects with feeding.

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Effect of wogonin, an active ingredient of Scutellariae Radix, on fish infected with Aeromonas hydrophila

Cui,

Zeng,

Guan

et al. 2024

Aquacult Int

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Paeonol increases the antioxidant and anti-inflammatory capacity of gibel carp (Carassius auratus gibelio) challenged with Aeromonas hydrophila

Cited by 7 publications

References 65 publications

Effects of glycyrrhetinic acid β on growth and virulence of Aeromonas hydrophila

Effects of glycyrrhetinic acid β on growth and virulence of Aeromonas hydrophila

The oral protective efficacy of magnolol against Aeromonas hydrophila and A. veronii infection via enhancing anti‐inflammatory ability in goldfish (Carassius auratus)

Effect of wogonin, an active ingredient of Scutellariae Radix, on fish infected with Aeromonas hydrophila

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