Genome-Wide Identification and Functional Analysis of Carboxylesterase and Methylesterase Gene Families in Peach (Prunus persica L. Batsch)

Abstract

Carboxylesterases (CXE) and methylesterases (MES) are hydrolytic enzymes that act on carboxylic esters and are involved in plant metabolic processes and defense responses. A few functions of plant CXE and MES genes have been identified but very little information is available about the role of most members. We made a comprehensive study of this gene family in a commercially important species, peach (Prunus persica L. Batsch). A total of 33 peach CXE genes and 18 MES genes were identified and shown to be distributed unevenly between the chromosomes. Based on phylogenetic analysis, CXEs and MESs clustered into two different branches. Comparison of the positions of intron and differences in motifs revealed the evolutionary relationships between CXE and MES genes. RNA-seq revealed differential expression patterns of CXE/MESs in peach flower, leaf, and ripening fruit and in response to methyl jasmonate (MeJA) and ultraviolet B treatment. Transcript levels of candidate genes were verified by real-time quantitative PCR. Heterologous expression in Escherichia coli identified three CXEs that were involved in the hydrolysis of volatile esters in vitro. Furthermore, two recombinant MES proteins were identified that could hydrolyze MeJA and methyl salicylate. Our results provide an important resource for the identification of functional CXE and MES genes involved in the catabolism of volatile esters, responses to biotic and abiotic stresses and activation of signaling molecules such as MeJA and methyl salicylate.

Genome-Wide Analysis of the Gene Structure, Expression and Protein Interactions of the Peach (Prunus persica) TIFY Gene Family

“…The raw RNA-Seq data were downloaded from the Sequence Read Archive (SRA) 6 database, with peach transcriptome data of flowers (PRJNA726283, three biological replicates of petal tissues at blooming stage of a 3-year-old F 2 individual generated from self-pollination of a peach accession “05-2-144,” Lu et al, 2021 ), roots and leaves (PRJEB12334, three biological replicates of root samples and leaf tissues from 15 1-year-old “GF277” peach trees and 15 1-year-old “Catherina” peach trees, respectively, Ksouri et al, 2016 ), shoots (PRJNA587386, three biological replicates from three different 5-year-old “Soomee” peach trees with three shoots each was used, Yu et al, 2020 ) and fruits (PRJNA576753, three biological replicates of fruit samples at stages S1, S2, S3, S4, and S5 of cv. “Hujingmilu” with five fruits each were used at each sample time, Cao et al, 2019 ). Adaptors and low-quality read filtering were conducted using fastp software v0.20.1 ( Chen et al, 2018 ).…”

“…S1, the first exponential growth stage; S2, the pit hardening stage; S3, the second exponential growth; S4, fruit ripening stage. The sampling times for stage S1 of "Maravilha" and "124 Pan" (15 days after full blooming) were the same with that of "Zao Huang Pan Tao" (Guo et al, 2018), but much earlier than that of "Hujingmilu" (34 days after full blooming, Cao et al, 2019). The y-axis represents the relative expression of TIFY members to the internal reference gene PpTEF2.…”

Genomic basis of high-altitude adaptation in Tibetan Prunus fruit trees

“…The RNA-seq was conducted on the Illumina NovaSeq 6000 platform, and 150-bp paired-end reads were generated (Data S1AM). The transcriptome data from the fruit produced by P. persica from a previously published study 116 were also used for transcriptome analysis.…”

Identification and Structure Analysis of KCS Family Genes Suggest Their Reponding to Regulate Fiber Development in Long-Staple Cotton Under Salt-Alkaline Stress

“…The 2000 bp DNA sequences upstream of GBKCSs gene were obtained from Cotton FGD (Cao et al, 2019;Malik et al, 2020). The cis-regulatory elements in the promoter region of the gene GBKCSs were predicted on the Plant CARE website (http:// bioinformatics.psb.ugent.be/webtools/plantcare/html/), choosing the hormone (IAA: auxin-AuxRR-core, ABA: abscisic acid-ABRE, SA: salicylic acid, MeJA: methyl jasmonate-CGTCA-motif, TGACG-motif), transcription factor binding, development, circadian control, defense and stress responsiveness, meristem expression related cis-acting elements to analysis.…”