Identification and Structure Analysis of KCS Family Genes Suggest Their Reponding to Regulate Fiber Development in Long-Staple Cotton Under Salt-Alkaline Stress

Rui, Cun; Chen, Xiugui; Xu, Nan; Wang, Jing; Zhang, Hong; Li, Shengmei; Huang, Hui; Fan, Yapeng; Zhang, Yuexin; Lu, Xuke; Wang, Delong; Gao, Wenwei; Ye, Wuwei

doi:10.3389/fgene.2022.812449

Cited by 15 publications

(12 citation statements)

References 60 publications

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“…VLCFA is an important precursor substance for the synthesis of plant cuticle waxes. KCS family genes, which encode the key restriction enzyme in the VLCFA synthesis pathway, have been identified in various plant species [4,27,28]. In this study, 20 XsKCS genes were identified in the yellow horn genome (Table 1).…”

Section: Discussionmentioning

confidence: 83%

Genome-Wide Identification and Expression Analysis of the KCS Gene Family in Yellow Horn Reveal Their Putative Function on Abiotic Stress Responses and Wax Accumulation

et al. 2022

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The β-ketoacyl CoA synthase encoded by the KCS genes is a rate-limiting enzyme for the synthesis of very-long-chain fatty acid (VLCFA), which catalyzes the VLCFA elongation. Yellow horn (Xanthoceras sorbifolium) is a horticultural tree species known for its kernel oil, which has strong resistance to drought, cold, high temperature, and saline-alkali. The conserved domain FAE1-CUT1-RPPA and ACP-syn-III_C of the KCS gene family were used to search the KCS sequences across the whole genomic sequence of yellow horn; a total of 20 XsKCS genes were identified and divided into four subfamilies. The conserved motif and transmembrane structure analysis revealed that most XsKCSs had a conserved transmembrane domain except XsKCS10 and XsKCS20. The prediction of cis-acting elements of XsKCS genes showed that XsKCS genes contained many stress and hormone response elements, such as ABRE, MBS, and LTR. Furthermore, XsKCS genes exhibited differential expression profiles under abiotic stress and stress-related hormone treatment conditions. Transcriptomic data showed that XsKCS1, XsKCS11, and XsKCS17 had higher expression in yellow horn with high leaf cuticular wax, indicating that they may function in the cuticular wax accumulation and drought response. This study laid a foundation for further functional verification of XsKCS genes in yellow horn stress response.

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Section: Discussionmentioning

confidence: 83%

Genome-Wide Identification and Expression Analysis of the KCS Gene Family in Yellow Horn Reveal Their Putative Function on Abiotic Stress Responses and Wax Accumulation

et al. 2022

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“…Glycerol-3-phosphate acyltransferase (GPAT), a key gene in glycerophospholipid metabolism, was increased 3–8-fold upon SLT (Figure S5D). The GPAT gene is associated with fatty acid unsaturation and plays a pivotal role in cold resistance in a number of plant species. , Plant 3-ketoacyl-CoA synthase (KCS), which is involved in the biosynthesis of very long chain fatty acids (VLCFAs), was increased 20-fold upon SLT stress training (Figure S5D). VLCFAs play important roles in biosynthesis of cuticular waxes, a hydrophobic film deposited on aerial tissue surfaces, and provides protection against pathogen infection .…”

Section: Resultsmentioning

confidence: 99%

Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

et al. 2023

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Under a changing climate, cultivating climate-resilient crops will be critical to maintaining food security. Here, we propose the application of reactive oxygen species (ROS)-generating nanoparticles as nanobiostimulants to trigger stress/immune responses and subsequently increase the stress resilience of plants. We established three regimens of silver nanoparticles (AgNPs)-based “stress training”: seed training (ST), leaf training (LT), and combined seed and leaf training (SLT). Trained rice seedlings were then exposed to either rice blast fungus (Magnaporthe oryzae) or chilling stress (10 °C). The results show that all “stress training” regimes, particularly SLT, significantly enhanced the resistance of rice against the fungal pathogen (lesion size reduced by 82% relative to untrained control). SLT also significantly enhanced rice tolerance to cold stress. The mechanisms for the enhanced resilience were investigated with metabolomics and transcriptomics, which show that “stress training” induced considerable metabolic and transcriptional reprogramming in rice leaves. AgNPs boosted ROS-activated stress signaling pathways by oxidative post-translational modifications of stress-related kinases, hormones, and transcriptional factors (TFs). These signaling pathways subsequently modulated the expression of defense genes, including specialized metabolites (SMs) biosynthesis genes, cell membrane lipid metabolism genes, and pathogen–plant interaction genes. Importantly, results showed that the “stress memory” can be transferred transgenerationally, conferring offspring seeds with improved seed germination and seedling vigor. This may provide an epigenetic breeding strategy to fortify stress resilience of crops. This nanobiostimulant-based stress training strategy will increase yield vigor against a changing climate and will contribute to sustainable agriculture by reducing agrochemical use.

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“…From previous studies, we have learned that some genes play important roles in fatty acid regulation. Taking KCS16 in the core network as an example, KCS16 is a member of the 3-ketoacyl CoA synthase family involved in very-long-chain fatty acid (VLCFA, C > 20) biosynthesis [ 38 ]. This gene is highly expressed in siliques and also substantially in leaves and other organs [ 39 ], and the enzyme has no in vitro elongation activity toward C16–C20 substrates [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of lncRNA–mRNA Regulatory Networks Related to Lipid Metabolism in High-Oleic-Acid Rapeseed

Wang

Zhao

et al. 2023

IJMS

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A high oleic acid content is considered an essential characteristic in the breeding of high-quality rapeseed in China. Long-chain non-coding RNA (lncRNA) molecules play an important role in the plant’s growth and its response to stress. To better understand the role of lncRNAs in regulating plant reproductive development, we analyzed whole-transcriptome and physiological data to characterize the dynamic changes in lncRNA expression during the four representative times of seed development of high- and low-oleic-acid rapeseed in three regions. We identified 21 and 14 lncRNA and mRNA modules, respectively. These modules were divided into three types related to region, development stages, and material. Next, we analyzed the key modules related to the oil content and the oleic acid, linoleic acid, and linolenic acid contents with physiological data and constructed the key functional network analysis on this basis. Genes related to lipid metabolism, such as 3-ketoacyl-CoA synthase 16 (KCS16) and acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1), were present in the co-expression network, suggesting that the effect of these genes on lipid metabolism might be embodied by the expression of these lncRNAs. Our results provide a fresh insight into region-, development-stage-, and material-biased changes in lncRNA expression in the seeds of Brassica napus. Some of these lncRNAs may participate in the regulatory network of lipid accumulation and metabolism, together with regulated genes. These results may help elucidate the regulatory system of lncRNAs in the lipid metabolism of high-oleic-acid rapeseed seeds.

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Identification and Structure Analysis of KCS Family Genes Suggest Their Reponding to Regulate Fiber Development in Long-Staple Cotton Under Salt-Alkaline Stress

Cited by 15 publications

References 60 publications

Genome-Wide Identification and Expression Analysis of the KCS Gene Family in Yellow Horn Reveal Their Putative Function on Abiotic Stress Responses and Wax Accumulation

Genome-Wide Identification and Expression Analysis of the KCS Gene Family in Yellow Horn Reveal Their Putative Function on Abiotic Stress Responses and Wax Accumulation

Engineering Climate-Resilient Rice Using a Nanobiostimulant-Based “Stress Training” Strategy

Integrated Analysis of lncRNA–mRNA Regulatory Networks Related to Lipid Metabolism in High-Oleic-Acid Rapeseed

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