Genome-Wide Identification and Functional Analysis of NADPH Oxidase Family Genes in Wheat During Development and Environmental Stress Responses

Abstract

As the key producers of reactive oxygen species (ROS), NADPH oxidases (NOXs), also known as respiratory burst oxidase homologs (RBOHs), play crucial roles in various biological processes in plants with considerable evolutionary selection and functional diversity in the entire terrestrial plant kingdom. However, only limited resources are available on the phylogenesis and functions of this gene family in wheat. Here, a total of 46 NOX family genes were identified in the wheat genome, and these NOXs could be classified into three subgroups: typical TaNOXs, TaNOX-likes, and ferric reduction oxidases (TaFROs). Phylogenetic analysis indicated that the typical TaNOXs might originate from TaFROs during evolution, and the TaFROs located on Chr 2 might be the most ancient forms of TaNOXs. TaNOXs are highly expressed in wheat with distinct tissue or organ-specificity and stress-inducible diversity. A large-scale expression and/or coexpression analysis demonstrated that TaNOXs can be divided into four functional groups with different expression patterns under a broad range of environmental stresses. Different TaNOXs are coexpressed with different sets of other genes, which widely participate in several important intracellular processes such as cell wall biosynthesis, defence response, and signal transduction, suggesting their vital but diversity of roles in plant growth regulation and stress responses of wheat.

Genome-wide identification and expression analysis of NADPH oxidase genes in response to ABA and abiotic stresses, and in fibre formation in Gossypium

“…These suggest that the Gossypium Rbohs are originated from a common ancester. The number of exons for Gossypium Rbohs was 10-15, similar to that in Rbohs in Arabidopsis, rice, maize, wheat, canola and soybean (Chang et al, 2016;Hu et al, 2018;Li et al, 2019a;Li et al, 2019b;Liu et al, 2019;Navathe et al, 2019). Additionally, the majority of the Gossypium Rbohs had the conserved EF hand domains, and NADPH_Ox, Ferric_reduct, FAD_binding_8 and NAD_binding_6 motifs of higher plants (Fig.…”

“…In the present study, we identified 13, 13, 26 and 19 Rbohs in G. arboretum, G. raimondii, G. hirsutum and G. barbadense, respectively ( Table 1). The number of these Rbohs in Gossypium, particularly in G. hirsutum and G. barbadense was larger than that in other plants such as Arabidopsis (10), rice (9), maize (14), Brachypodium distachyon (9), Populus trichocarpa (10), wheat (19), Brassica rapa (14) and soybean (17) (Chang et al, 2016;Hu et al, 2018;Li et al, 2019a;Li et al, 2019b;Liu et al, 2019;Navathe et al, 2019), implying that more sophisticated ROS signal transduction mechanisms may exist in Gossypium. Compared with other crops as mentioned above, Gossypium plants are more tolerant to environmental stresses like drought, high salt, heat stress, and so forth.…”

“…In recent years, Rboh gene families have been characterized at genome-wide levels in many plants such as Arabidopsis, rice, wheat, Glycine max, Brassica rapa and multiple fruit trees. The expression patterns of the genes were also studied in tissues and in responding to diverse stresses (Chang et al, 2016;Hu et al, 2018;Cheng et al, 2019;Li et al, 2019a;Li et al, 2019b;Liu et al, 2019;Navathe et al, 2019;Zou, Yang & Zhang, 2019). Moreover, 2 RbohBs, 5 RbohDs (3 are RbohKs according to our classification) and 4 RbohFs in cotton have shown to be differentially expressed after infection by Verticillium dahliae (Li et al, 2019a;Li et al, 2019b).…”

Mapping Agronomic and Quality Traits in Elite Durum Wheat Lines under Differing Water Regimes

“…These genes are both related with photosynthesis. Hu et al [112] highlighted that NADPH plays a crucial role in biological processes in plants, such as the regulation of the production of reactive oxygen species (ROS) for the stress tolerance [113,114]. Additional GWAS analyses using reduced datasets revealed other interesting genes for this QTL (chromosome 2A, Supplementary Materials Table S8), encoding for the Acyl-CoA N-acyltransferase and the chloroplastic zeaxanthin epoxidase.…”

GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm

“…Two TaNOX genes have been reported to confer resistance to brown rust infection 40 . To date, 15 TaNOXs have been reported to be co-expressed with different sets of other genes that participate in several critical intracellular processes such as cell wall biosynthesis, defence response, and signal transduction, suggesting vital but diverse roles for these genes in regulating plant growth and stress responses in wheat 43 . Two of these genes (TaNOX8 and TaNOX9) were mapped to chromosome 4DL.…”