Genome-wide identification and expression analysis of NADPH oxidase genes in response to ABA and abiotic stresses, and in fibre formation in Gossypium

Abstract

Plasma membrane NADPH oxidases, also named respiratory burst oxidase homologues (Rbohs), play pivotal roles in many aspects of growth and development, as well as in responses to hormone signalings and various biotic and abiotic stresses. Although Rbohs family members have been identified in several plants, little is known about Rbohs in Gossypium. In this report, we characterized 13, 13, 26 and 19 Rbohs in G. arboretum, G. raimondii, G. hirsutum and G. barbadense, respectively. These Rbohs were conservative in physical properties, structures of genes and motifs. The expansion and evolution of the Rbohs dominantly depended on segmental duplication, and were under the purifying selection. Transcription analyses showed that GhRbohs were expressed in various tissues, and most GhRbohs were highly expressed in flowers. Moreover, different GhRbohs had very diverse expression patterns in response to ABA, high salinity, osmotic stress and heat stress. Some GhRbohs were preferentially and specifically expressed during ovule growth and fiber formation. These results suggest that GhRbohs may serve highly differential roles in mediating ABA signaling, in acclimation to environmental stimuli, and in fiber growth and development. Our findings are valuable for further elucidating the functions and regulation mechanisms of the Rbohs in adaptation to diverse stresses, and in growth and development in Gossypium.

Comprehensive Analysis of Respiratory Burst Oxidase Homologs (Rboh) Gene Family and Function of GbRboh5/18 on Verticillium Wilt Resistance in Gossypium barbadense

“…We systematically identified 87 Rboh genes in these representative five sequenced Gossypium species: G. arboretum, G. raimondii, G. australe, G. hirsutum, and G. barbadense. Both G. arboretum and G. raimondii harbored 13 Rboh genes, a result that agrees with the findings of Zhang et al (2020). The main difference with that work is that we found 24 and 27 Rboh genes in G. hirsutum and G. barbadense, while they found 26 and 19, respectively (Wang et al, 2020).…”

UDP-Glucose Dehydrogenases: Identification, Expression, and Function Analyses in Upland Cotton (Gossypium hirsutum)

“…Studies have shown that shoot ABA application promotes root growth by accelerating both cell division and elongation of the root tips ( Lin and Kao, 2001 ). ABA synthesis regulates fiber development ( Zhang G. et al, 2020 ). Therefore, we chose GH_D12G1806 as our candidate genes for the next step of functional verification.…”

Genome-wide analysis of PRR gene family uncovers their roles in circadian rhythmic changes and response to drought stress in Gossypium hirsutum L.

“…In addition, there are many studies focus on the response of circadian clock to abiotic stress in crops ( Flowers, 2004 ; Lu et al, 2017 ; Zhang et al, 2020 ). TOC1 can bind to the ABAR promoter of ABA-related genes and regulate its circadian rhythm expression, and can be thought to act as molecular switches between drought stress signaling pathways and circadian clocks in Arabidopsis ( Legnaioli, Cuevas & Mas, 2009 ).…”