Genome-wide expression changes induced by bisphenol A, F and S in human stem cell derived hepatocyte-like cells

Lucendo‐Villarin, Baltasar; Nell, Patrick; Hellwig, Birte; Filis, Panagiotis; Feuerborn, David; O’Shaughnessy, Peter J.; Godoy, Patrício; Rahnenführer, Jörg; Hengstler, Jan G.; Cherianidou, Anna; Sachinidis, Agapios; Fowler, Paul A.; Hay, David C.

doi:10.17179/excli2020-2934

Cited by 2 publications

(1 citation statement)

References 57 publications

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“…Loyd et al ( 2019 ) found that BPA increased TP53 protein expression in the breast carcinoma T-47D cell line at a concentration range of 200 nM to 2 µM, in and in the breast adenocarcinoma MCF-7 cell line at a concentration range of 1–2 µM (Lloyd et al 2019 ). Similar results were obtained by Lucendo-Villarin et al ( 2020 ), who showed that BPA, BPS, and BPF increased TP53 mRNA expression in the human pluripotent stem cell lines (Man12 (male)) (Lucendo-Villarin et al 2020 ). Consistent results regarding elevated TP53 protein expression were also acquired in the mouse-derived C17.2 multipotent neural stem cell line treated with TBBPA at concentrations ranging from 12.5 to 50 µM (Cho et al 2020 ).…”

Section: Discussionsupporting

confidence: 84%

Toxicity of bisphenol A (BPA) and its derivatives in divers biological models with the assessment of molecular mechanisms of toxicity

Rybczyńska‐Tkaczyk

Skóra

Szychowski

2023

Environ Sci Pollut Res

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The aim of the study was to determine totoxicity of bisphenol A (BPA) and its derivatives (bisphenol S (BPS), bisphenol F (BPF), and tetrabromobisphenol A (TBBPA)) due to its high accumulation in environment. The performed analysis revealed the toxicity of the BPA, BPF, and BPS against Kurthia gibsoni, Microbacterium sp., and Brevundimonas diminuta as the most sensitive, reaching microbial toxic concentrations in the range of 0.018–0.031 mg ∙ L−1. Moreover, the genotoxicity assay shows the ability of all tested compounds to increase in the β-galactosidase level at the concentration range 7.81–500 µM (in Escherichia coli, PQ37). In turn, the matbolic activation of tested bishpenols has caused the enhacement of the genotoxicity and cytotoxicity effect. Interestingely, the highest phytotoxicity effect was pointed for BPA and TBBPA at the concentrations of 10 mg ∙ L−1 and 50 mg ∙ L−1, which cause the inhibition of root growth by 58% and 45%, respectively (especially for S. alba and S. saccharatum). Furthermore, the cytotoxicity analyses show the ability of BPA, BPS, and TBBPA to significantly decrease the metabolic activity of human keratynoctes in vitro after 24 h of treatment at the micromolar concentrations. Simialry, the impact of the certain bisphenols on proliferation-, apoptosis-, and inflammation-related mRNA expression was shown in tested cell line. Summarizing, the presented results have proved that BPA and its derrivatives are able to show high negative effect on certain living orgnisms such as bacteria, plants, and human cells, which is strict related to pro-apoptotic and genotoxic mechanism of action.

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Section: Discussionsupporting

confidence: 84%

Toxicity of bisphenol A (BPA) and its derivatives in divers biological models with the assessment of molecular mechanisms of toxicity

Rybczyńska‐Tkaczyk

Skóra

Szychowski

2023

Environ Sci Pollut Res

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Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes

et al. 2021

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Summary This protocol describes the production of hepatocyte-like cells (HLCs) from human pluripotent stem cells and how to induce hepatic steatosis, a condition characterized by intracellular lipid accumulation. Following differentiation to an HLC phenotype, intracellular lipid accumulation is induced with a steatosis induction cocktail, allowing the user to examine the cellular processes that underpin hepatic steatosis. Furthermore, the renewable nature of our system, on a defined genetic background, permits in-depth mechanistic analysis, which may facilitate therapeutic target identification in the future. For complete details on the use and execution of this protocol, please refer to Sinton et al. (2021) .

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Genome-wide expression changes induced by bisphenol A, F and S in human stem cell derived hepatocyte-like cells

Cited by 2 publications

References 57 publications

Toxicity of bisphenol A (BPA) and its derivatives in divers biological models with the assessment of molecular mechanisms of toxicity

Toxicity of bisphenol A (BPA) and its derivatives in divers biological models with the assessment of molecular mechanisms of toxicity

Modeling human hepatic steatosis in pluripotent stem cell-derived hepatocytes

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