Genome-wide dissection of Fusarium resistance in tomato reveals multiple complex loci

Sela-Buurlage, Marianne B.; Budai-Hadrian, Ofra; Pan, Qilin; Carmel-Goren, Lea; Vunsch, R; Zamir, Dani; Fluhr, Robert

doi:10.1007/s004380100509

Cited by 72 publications

(51 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…lycopersici race 0 (Fol:0, ex race 1) was introgressed from S. pimpinellifolium . The I locus was mapped on the short arm of chromosome 11 (Ori et al 1997; Sela-Buurlage et al 2001; Scott et al 2004) positioned between the RFLP markers TG523 and TG7 (approximate interval of 3.8 cM). Sequence data, from both ends of these RFLP fragments, were available and these were used to design primers to amplify fragments in resistant and susceptible varieties that can be screened for polymorphisms associated with the resistance.…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Arens¹,

Mansilla

Deinum³

et al. 2009

Theor Appl Genet

View full text Add to dashboard Cite

Molecular markers linked to phenotypically important traits are of great interest especially when traits are difficult and/or costly to be observed. In tomato where a strong focus on resistance breeding has led to the introgression of several resistance genes, resistance traits have become important characteristics in distinctness, uniformity and stability (DUS) testing for Plant Breeders Rights (PBR) applications. Evaluation of disease traits in biological assays is not always straightforward because assays are often influenced by environmental factors, and difficulties in scoring exist. In this study, we describe the development and/or evaluation of molecular marker assays for the Verticillium genes Ve1 and Ve2, the tomato mosaic virusTm1 (linked marker), the tomato mosaic virus Tm2 and Tm22 genes, the Meloidogyne incognita Mi1-2 gene, the Fusarium I (linked marker) and I2 loci, which are obligatory traits in PBR testing. The marker assays were evaluated for their robustness in a ring test and then evaluated in a set of varieties. Although in general, results between biological assays and marker assays gave highly correlated results, marker assays showed an advantage over biological tests in that the results were clearer, i.e., homozygote/heterozygote presence of the resistance gene can be detected and heterogeneity in seed lots can be identified readily. Within the UPOV framework for granting of PBR, the markers have the potential to fulfil the requirements needed for implementation in DUS testing of candidate varieties and could complement or may be an alternative to the pathogenesis tests that are carried out at present.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-009-1183-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Arens¹,

Mansilla

Deinum³

et al. 2009

Theor Appl Genet

View full text Add to dashboard Cite

show abstract

“…The I gene originates from Solanum [Lycopersicon] pimpinellifolium and resides on chromosome 11 [12],[13], while the I-1 gene is located on chromosome 7 in another wild relative of tomato, Solanum [Lycopersicon] pennellii [14]. The I-2 gene has been cloned and encodes an R protein of the common NB-LRR class [15].…”

Section: Introductionmentioning

confidence: 99%

Suppression of Plant Resistance Gene-Based Immunity by a Fungal Effector

2008

View full text Add to dashboard Cite

The innate immune system of plants consists of two layers. The first layer, called basal resistance, governs recognition of conserved microbial molecules and fends off most attempted invasions. The second layer is based on Resistance (R) genes that mediate recognition of effectors, proteins secreted by pathogens to suppress or evade basal resistance. Here, we show that a plant-pathogenic fungus secretes an effector that can both trigger and suppress R gene-based immunity. This effector, Avr1, is secreted by the xylem-invading fungus Fusarium oxysporum f.sp. lycopersici (Fol) and triggers disease resistance when the host plant, tomato, carries a matching R gene (I or I-1). At the same time, Avr1 suppresses the protective effect of two other R genes, I-2 and I-3. Based on these observations, we tentatively reconstruct the evolutionary arms race that has taken place between tomato R genes and effectors of Fol. This molecular analysis has revealed a hitherto unpredicted strategy for durable disease control based on resistance gene combinations.

show abstract

“…Four I2 homologues [ I2 [GenBank: AF118127], I2C-1 [AF004878], I2C-2 [AF004879], I2C-5 [AF408704]], originated from wild tomato species S. pimpinellifilium were downloaded from GenBank [21, 37, 38]. Two partial genes I2C-3 and I2C-4 were excluded from this study.…”

Section: Methodsmentioning

confidence: 99%

The I2 resistance gene homologues in Solanum have complex evolutionary patterns and are targeted by miRNAs

et al. 2014

View full text Add to dashboard Cite

BackgroundSeveral resistance traits, including the I2 resistance against tomato fusarium wilt, were mapped to the long arm of chromosome 11 of Solanum. However, the structure and evolution of this locus remain poorly understood.ResultsComparative analysis showed that the structure and evolutionary patterns of the I2 locus vary considerably between potato and tomato. The I2 homologues from different Solanaceae species usually do not have orthologous relationship, due to duplication, deletion and frequent sequence exchanges. At least 154 sequence exchanges were detected among 76 tomato I2 homologues, but sequence exchanges between I2 homologues in potato is less frequent. Previous study showed that I2 homologues in potato were targeted by miR482. However, our data showed that I2 homologues in tomato were targeted by miR6024 rather than miR482. Furthermore, miR6024 triggers phasiRNAs from I2 homologues in tomato. Sequence analysis showed that miR6024 was originated after the divergence of Solanaceae. We hypothesized that miR6024 and miR482 might have facilitated the expansion of the I2 family in Solanaceae species, since they can minimize their potential toxic effects by down-regulating their expression.ConclusionsThe I2 locus represents a most divergent resistance gene cluster in Solanum. Its high divergence was partly due to frequent sequence exchanges between homologues. We propose that the successful expansion of I2 homologues in Solanum was at least partially attributed to miRNA mediated regulation.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-743) contains supplementary material, which is available to authorized users.

show abstract

Genome-wide dissection of Fusarium resistance in tomato reveals multiple complex loci

Cited by 72 publications

References 23 publications

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing

Suppression of Plant Resistance Gene-Based Immunity by a Fungal Effector

The I2 resistance gene homologues in Solanum have complex evolutionary patterns and are targeted by miRNAs

Contact Info

Product

Resources

About