Genome-Wide Differential DNA Methylomes Provide Insights into the Infertility of Triploid Oysters

Sun, Dongfang; Yu, Hong; Li, Qi

doi:10.1007/s10126-021-10083-y

Cited by 5 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nucleotide excision repair pathway is a pluripotent pathway that recognizes and removes various types of DNA lesions induced by environmental sources to counteract their deleterious effects [45]. A study on triploid oysters revealed that hypermethylated genes involved in nucleotide excision repair may have functional significance in producing gametes during both meiosis divisions in M-3nα [46]. The ubiquitin proteolytic system plays a critical role in several basic cellular processes, such as regulating the cell cycle, modulating immune and inflammatory responses, controlling signal transduction pathways, and facilitating development and differentiation [47].…”

Section: Analysis Of Kegg Enrichment Pathwaysmentioning

confidence: 99%

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Ma,

Ye,

Yao

et al. 2023

Fishes

View full text Add to dashboard Cite

In the cultivation of Mytilus unguiculatus, the broodstock are shade-dried to stimulate sperm and egg production. To identify the functional genes affecting gonad development in M. unguiculatus, the transcriptome of gonads in mussels stimulated by shade-drying and those not stimulated were compared. Differential gene expression analysis revealed that there were 22 differentially expressed genes (DEGs) in the testis and 70 DEGs in the ovary among the experimental groups. KEGG enrichment analysis identified a total of 11 pathways that might be related to environmental stimuli affecting gonadal development. Nicotinic acetylcholine receptors (AChRs), the cholecystokinin A receptor (CCKAR), hypocretin (orexin) receptor 2 (HCRTR2), and gamma-aminobutyric acid type B receptor (GABBR) were highly expressed in the neuroactive ligand-receptor interaction pathway, indicating that these genes might be involved in the transduction of environmental information that stimulates gonadal development. Meanwhile, nuclear receptor co-repressor 2 (NCoR2) was highly expressed in the notch signaling pathway, indicating that NCoR2 might be involved in the regulation of gonad development. To validate the transcriptome data, we selected five DEGs in the KEGG signaling pathway, including AChRs, CCKAR, HCRTR2, GABAB, and NCoR2, for real-time quantitative PCR (RT-qPCR), which produced results consistent with the RNA-Seq data. The transcriptome analysis and gene pathway identification in this study have enhanced our comprehension of the reproductive mechanisms in M. unguiculatus.

show abstract

Section: Analysis Of Kegg Enrichment Pathwaysmentioning

confidence: 99%

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Ma,

Ye,

Yao

et al. 2023

Fishes

View full text Add to dashboard Cite

show abstract

“…epigenetically marked locus was identified in the sex determination region (SDR) Yang et al(2022) OysterMethylomes are related to infertility of triploid oystersSun et al (2022) …”

mentioning

confidence: 99%

Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture

2022

View full text Add to dashboard Cite

Major progress has been made with genomic and genetic studies in aquaculture in the last decade. However, research on epigenetic regulation of aquaculture traits is still at an early stage. It is apparent that most, if not all, aquaculture traits are regulated at both genetic and epigenetic levels. This paper reviews recent progress in understanding of genetic and epigenetic regulation of important aquaculture traits such as growth, reproduction, disease resistance, and stress responses. Although it is challenging to make generalized statements, DNA methylation is mostly correlated with down-regulation of gene expression, especially when at promoters and enhancers. As such, methylation of growth factors and their receptors is negatively correlated with growth; hypomethylation of genes important for stress tolerance is correlated with increased stress tolerance; hypomethylation of genes important for male or female sex differentiation leads to sex differentiation into males or females, respectively. It is apparent that environmental regulation of aquaculture traits is mediated at the level of epigenetic regulation, and such environment-induced epigenetic changes appeared to be intergenerationally inherited, but evidences for transgenerational inheritance are still limited.

show abstract

Examination of the role of CgSox-like in sex determination and gonadal development in the Pacific oyster Crassostrea gigas

Sun

2023

Aquaculture

View full text Add to dashboard Cite

Genome-Wide Differential DNA Methylomes Provide Insights into the Infertility of Triploid Oysters

Cited by 5 publications

References 57 publications

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture

Examination of the role of CgSox-like in sex determination and gonadal development in the Pacific oyster Crassostrea gigas

Contact Info

Product

Resources

About