Examination of the role of CgSox-like in sex determination and gonadal development in the Pacific oyster Crassostrea gigas

Sun, Dongfang; Yu, Hong; Li, Qi

doi:10.1016/j.aquaculture.2023.739234

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Sperm had a more dramatic methylation response to elevated pCO 2 , with 2,916 DML compared to 89 in female gonad tissue oysters, even though a similar number of CpGs with 10x coverage were detected in both sexes. Sex-specific differences in methylation [46][47][48] are well-documented in C. gigas. Male C. gigas have higher levels of gene body methylation than females in both somatic [48] and reproductive tissue [46,47].…”

Section: Methylation Regulates Gene Activity Differently In Female An...mentioning

confidence: 99%

“…Sex-specific differences in methylation [46][47][48] are well-documented in C. gigas. Male C. gigas have higher levels of gene body methylation than females in both somatic [48] and reproductive tissue [46,47]. Our results confirm that these patterns are present in C. virginica gonads as well.…”

Section: Methylation Regulates Gene Activity Differently In Female An...mentioning

confidence: 99%

“…reproductive tissue [38,40] by examining sex-specific effects of OA on reproductive tissue responses. As female and male oysters undergo distinct gametogenic processes, and egg and sperm have unique roles in reproduction, it follows that there are sex-specific methylation [46][47][48] and gene expression [49] patterns in reproductive tissue that would confound findings if analyzed together. We compared female gonad tissue and sperm exposed to control and elevated pCO 2 conditions to investigate 1) the influence of genetic variation on methylation and gene expression; 2) the impact of OA on changes in gene activity and methylation; and 3) the functional role of DNA methylation in regulating gene activity.…”

Section: Introductionmentioning

confidence: 99%

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DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)

Venkataraman,

Huffmyer,

White

et al. 2024

Preprint

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Ocean acidification significantly affects marine calcifiers like oysters, warranting the study of molecular mechanisms that contribute to adaptive plasticity in response to environmental change like DNA methylation. However, a consensus has not been reached on the extent to which methylation modules gene expression, and in turn plasticity, in marine invertebrates. To this end, we investigated the impact of pCO2on gene expression and DNA methylation in the eastern oyster,Crassostrea virginica. After a 30-day exposure to control (572 ppm) or elevated pCO2(2,827 ppm), whole genome bisulfite sequencing (WGBS) and RNA-Seq data were generated from adult female gonad and male sperm samples. Although differentially methylated loci (DML) were identified in female (89) and male (2,916) gonad tissues, there were no differentially expressed genes, and only one differentially expressed transcript in females. However, gene body methylation impacted other transcript-level processes in sperm, such as the maximum number of transcripts expressed per gene and shifts in the predominant transcript. Elevated pCO2exposure increased gene expression variability (transcriptional noise) in males but decreased noise in females, suggesting a sex-specific role of methylation in gene expression regulation. Functional annotation of genes with changes in transcript-level expression or containing DML revealed several enriched biological processes potentially involved in elevated pCO2response, including apoptotic pathways and signal transduction, as well as reproductive functions. Taken together, these results suggest that DNA methylation may regulate gene expression variability to maintain homeostasis in elevated pCO2conditions and could play a key role in environmental resilience in marine invertebrates.

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Section: Methylation Regulates Gene Activity Differently In Female An...mentioning

confidence: 99%

Section: Methylation Regulates Gene Activity Differently In Female An...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)

Venkataraman,

Huffmyer,

White

et al. 2024

Preprint

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“…Additionally, they have identified known functional genes through transcriptome sequencing. For example, Sun et al [28] discovered a novel HMGbox-containing homolog of the Sox gene in Crassostrea gigas (named CgSox-like), which was found to be involved in the sex determination or gonadal development process in male oysters. Lian [29] selected several genes related to sex determination and differentiation in Tapes conspersus, including Zglp1, Foxl2, Tssk1, and Spag6, and showed the effect of their expression on gonadal development.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Ma,

Ye,

Yao

et al. 2023

Fishes

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In the cultivation of Mytilus unguiculatus, the broodstock are shade-dried to stimulate sperm and egg production. To identify the functional genes affecting gonad development in M. unguiculatus, the transcriptome of gonads in mussels stimulated by shade-drying and those not stimulated were compared. Differential gene expression analysis revealed that there were 22 differentially expressed genes (DEGs) in the testis and 70 DEGs in the ovary among the experimental groups. KEGG enrichment analysis identified a total of 11 pathways that might be related to environmental stimuli affecting gonadal development. Nicotinic acetylcholine receptors (AChRs), the cholecystokinin A receptor (CCKAR), hypocretin (orexin) receptor 2 (HCRTR2), and gamma-aminobutyric acid type B receptor (GABBR) were highly expressed in the neuroactive ligand-receptor interaction pathway, indicating that these genes might be involved in the transduction of environmental information that stimulates gonadal development. Meanwhile, nuclear receptor co-repressor 2 (NCoR2) was highly expressed in the notch signaling pathway, indicating that NCoR2 might be involved in the regulation of gonad development. To validate the transcriptome data, we selected five DEGs in the KEGG signaling pathway, including AChRs, CCKAR, HCRTR2, GABAB, and NCoR2, for real-time quantitative PCR (RT-qPCR), which produced results consistent with the RNA-Seq data. The transcriptome analysis and gene pathway identification in this study have enhanced our comprehension of the reproductive mechanisms in M. unguiculatus.

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Anesthetic sampling and the tissue regeneration potential of oysters: A study with Crassostrea hongkongensis

Bai,

Wei,

Guan

et al. 2024

Aquaculture Reports

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Examination of the role of CgSox-like in sex determination and gonadal development in the Pacific oyster Crassostrea gigas

Cited by 6 publications

References 42 publications

DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)

DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Anesthetic sampling and the tissue regeneration potential of oysters: A study with Crassostrea hongkongensis

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Examination of the role of CgSox-like in sex determination and gonadal development in the Pacific oyster Crassostrea gigas

Cited by 6 publications

References 42 publications

DNA methylation correlates with transcriptional noise in response to elevated pCO2in the eastern oyster (Crassostrea virginica)

DNA methylation correlates with transcriptional noise in response to elevated pCO2in the eastern oyster (Crassostrea virginica)

Transcriptome Sequencing Analysis of Sex-Related Genes in the Gonads of Mytilus unguiculatus

Anesthetic sampling and the tissue regeneration potential of oysters: A study with Crassostrea hongkongensis

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Product

Resources

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DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)

DNA methylation correlates with transcriptional noise in response to elevated pCO₂in the eastern oyster (Crassostrea virginica)