Genome-wide characterization and expression profiling ofNACtranscription factor genes under abiotic stresses in radish (Raphanus sativusL.)

Abstract

NAC (NAM, no apical meristem; ATAF, Arabidopsis transcription activation factor and CUC, cup-shaped cotyledon) proteins are among the largest transcription factor (TF) families playing fundamental biological processes, including cell expansion and differentiation, and hormone signaling in response to biotic and abiotic stresses. In this study, 172 RsNACs comprising 17 membrane-bound members were identified from the whole radish genome. In total, 98 RsNAC genes were non-uniformly distributed across the nine radish chromosomes. In silico analysis revealed that expression patterns of several NAC genes were tissue-specific such as a preferential expression in roots and leaves. In addition, 21 representative NAC genes were selected to investigate their responses to heavy metals (HMs), salt, heat, drought and abscisic acid (ABA) stresses using real-time polymerase chain reaction (RT-qPCR). As a result, differential expressions among these genes were identified where RsNAC023 and RsNAC080 genes responded positively to all stresses except ABA, while RsNAC145 responded more actively to salt, heat and drought stresses compared with other genes. The results provides more valuable information and robust candidate genes for future functional analysis for improving abiotic stress tolerances in radish.

Comprehensive genomic characterization of NAC transcription factor family and their response to salt and drought stress in peanut

“…The number of NAC genes in cultivated peanut (164) was larger than that in other plant species (for example, 105 in Arabidopsis [40], 141 in rice [41], and 101 in soybean [46]), which was approximately 1.56-fold than that in Arabidopsis, and a similar result was found in Populus [10]. The NAC gene density in A.duranensis, A. ipaensis and A.hypogaea (0.07/Mb, 0.05/Mb, 0.06/Mb) was lower than that in Arabidopsis (0.87/Mb) and rice (0.37/Mb) [11]. This may be attributed to Arachis large genome sizes, which suggested that the genome size and number of NAC family members were not always correlated.…”

“…2), suggesting that diversification and expansion of this subgroup occurred after the monocot-dicot divergence. This phenomenon has also been found in radish, Populus and other species [10,11].…”

“…NAC proteins typically have a conserved NAM domain at the N-terminus and a highly variable domain at the Cterminus, the latter of which is related to specific biological functions. NAC family genes have been studied extensively in a variety of plant species, including gymnosperms and embryophytes [10][11][12][13][14][15][16][17][18][19]. However, until recently, comprehensive analyses of peanut NAC family genes and their response patterns to salt and drought stresses have been limited.…”

Root Growth Adaptation to Climate Change in Crops

“…In several crops, specific families of transcription factors are candidates to mediate heat stress response in roots. Thus, HD-ZIP and NAC transcription factors are induced by heat stress in potato and radish (Karanja et al, 2017;Li et al, 2019). In batata, ABF4, an ABA-responsive element binding factor that is up-regulated under heat stress promotes the expression of several stress responsive genes and mediate root elongation response .…”

Analysis of NAC Domain Transcription Factor Genes of Tectona grandis L.f. Involved in Secondary Cell Wall Deposition

“…Comparative genomic and gene functional analyses have indicated that NAC TFs underwent an expansion in the lineage of vascular plants [13]. Also, tracheid evolution is associated with a burst of structural diversity [14], which is probably related to NAC genes controlling SCW biosynthesis in vascular plants, comprising seedless vascular plants, gymnosperms, and angiosperms [15]. Noticeably, plant species that are structurally less complex contain significantly fewer NAC genes, such as the model bryophyte Physcomitrella patens (Hedw.)…”