Genome-wide association study for semen quality traits in German Warmblood stallions

Gottschalk, Maren; Metzger, Julia; Martinsson, Gunilla; Sieme, Harald; Distl, O.

doi:10.1016/j.anireprosci.2016.06.002

Cited by 25 publications

(26 citation statements)

References 38 publications

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“…Most studies on CRISP‐3 in stallion semen associate upregulation of the protein with increased fertility, whereas in our study upregulation was associated with decreased sperm survival during storage. Based on genomic studies, CRISP‐3 is associated with stallion fertility and semen quality traits (Hamann et al, , Novak et al, , and Gottschalk, Metzger, Martinsson, Sieme, & Distl, ). In raw semen, high levels of CRISP‐3 in SP result in higher sperm motility and improved semen quality index (Restrepo, Rojano, & Usuga, ), but in our study, higher levels of CRISP‐3 decreased sperm longevity during storage.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of CRISP‐3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage

Kareskoski

Palviainen

Johannisson

et al. 2020

Reprod Domestic Animals

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For unknown reasons, stallion fertility and sperm longevity during cooled storage of semen vary markedly between individuals. Spermatozoa from individual stallions react differently to the presence, or the removal, of seminal plasma (SP). The aim was to evaluate differences in protein content in stallion seminal plasma with either a positive or a negative effect on sperm chromatin integrity during storage. Stallion semen samples from different ejaculate fractions were stored at 5°C for 24 hr. Sperm survival was assessed after storage using a sperm chromatin structure assay. Protein expression in SP with either positive or negative effects on sperm survival during storage was studied using two‐dimensional differential gel electrophoresis and liquid chromatography–mass spectrometry. Lower sperm chromatin integrity was associated with upregulation of the proteins kallikrein, CRISP‐3 and HSP‐1, while higher chromatin integrity was associated with upregulation of TIMP‐2. In the sperm‐rich fractions, kallikrein and CRISP‐3 differed significantly between SP samples with differing effects on sperm chromatin integrity. In the sperm‐poor fractions, TIMP‐2 and HSP‐1 differed significantly between the two SP groups. Differences in the seminal plasma proteome are associated with sperm longevity during cooled storage.

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Section: Discussionmentioning

confidence: 99%

Upregulation of CRISP‐3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage

Kareskoski

Palviainen

Johannisson

et al. 2020

Reprod Domestic Animals

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show abstract

“…Different studies have proposed candidate genes with functional importance for stallion fertility including CRISP‐3 (Gottschalk et al, ). Some polymorphic sites of this gene have been related to fertility and even to cryotolerance of stallion sperm (Hamann et al, ; Jobim et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…A genomewide association study for semen quality traits included the CRISP‐3 gene among the candidate genes influencing the fertility of German Warmblood stallions; specifically, the CRISP‐3 gene on ECA20 chromosomal position was associated with sperm concentration (Gottschalk et al, ). In the current study, an association was found among all the nonsynonymous polymorphisms evaluated of the CRISP‐3 gene, with the seminal quality expressed through MOT, SV, NM or MI of the spermatozoa (Table ).…”

Section: Discussionmentioning

confidence: 99%

“…Few regression coefficients were significant ( p < 0.05); however, at least one of the CRISP‐3 polymorphisms showed a positive or negative effect on MOT, SV and MI (Table ). Gottschalk et al () in a study where genes encoding cysteine‐rich secretory proteins were included found that the allele substitution effects were partially positive or negative for traits of semen quality and all highly correlated SNPs showed the same sign for the allele substitution effects.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, nonsynonymous polymorphism of CRISP‐3 gene has been found to correlate with first‐cycle conception rates in stallions (Hamann et al, ), and we have recently reported a relationship between CRISP‐3 genotype and seminal plasma protein and post‐thawing semen quality in stallions (Usuga, Rojano, & Restrepo, ). Additionally, a recent genomewide association study for semen quality traits included the CRISP‐3 gene among the candidate genes influencing stallion fertility (Gottschalk, Metzger, Martinsson, Sieme, & Distl, ). However, the role of the CRISP‐3 gene through the expression of its protein in the SP of raw semen quality is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Betancur

Rojano

Suarez

2018

Reprod Domestic Animals

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Cysteine-rich secretory protein-3 (CRISP-3) and some of its nonsynonymous polymorphism have been related to the fertility and freezability of stallion semen; however, the role of the CRISP-3 gene and its seminal plasma protein in the raw semen quality is still unknown. The aim of this study was to evaluate the relationship of CRISP-3 with semen quality in stallions. DNA was obtained from blood samples of 100 stallions, from which 30 stallions were randomly selected to obtain 60 ejaculates. Through PCR amplification and sequencing, the variation of four nonsynonymous SNPs from CRISP-3 was identified and haplotypes were derived. Semen quality was assessed through the total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI) and a seminal quality index (SQi). CRISP-3 protein content of seminal plasma (SP) was determined by ELISA. The effect of the genotype, the haplotype and the concentration of the CRISP-3 protein on the seminal quality were evaluated through generalized linear models and linear regression analyses. Homozygous genotypes for SNP1, SNP2 and SNP3 and the heterozygous genotype for SNP4 showed a positive effect on seminal quality. Different haplotypes with positive effect on MOT, SV, NM, MI and SQi were identified. The allelic substitution analysis resulted in positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3). A high level of CRISP-3 resulted in a higher MOT and SQi. It is concluded that the quality of stallion semen is influenced by the genotype of CRISP-3 and the concentration of CRISP-3 protein in SP.

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Künstliche Besamung bei Haus- und Wildtierarten

Waberski

2023

Biotechnologie Bei Nutztieren 1

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Genome-wide association study for semen quality traits in German Warmblood stallions

Cited by 25 publications

References 38 publications

Upregulation of CRISP‐3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage

Upregulation of CRISP‐3 and kallikrein in stallion seminal plasma is associated with poor tolerance of cooled storage

Relationship of cysteine‐rich secretory protein‐3 gene and protein with semen quality in stallions

Künstliche Besamung bei Haus- und Wildtierarten

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