Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa

Lan, Ting; Gao, Jie; Zeng, Qing‐Yin

doi:10.1007/s11295-012-0551-2

Cited by 86 publications

(109 citation statements)

References 33 publications

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“…Among the OsLEAs, BdLEAs, ZmLEAs, and SbLEAs, only one or two LEA proteins show GRAVY values larger than 0 (Fig 2d, S1 Table). This is similar as those reported in dicots previously [8,17], suggesting the apparently hydrophobic characteristic of LEA proteins in plants.…”

Section: Resultssupporting

confidence: 92%

“…Additionally, the LEA gene number of O . sativa varies in different studies [17, 18]. Therefore, we re-searched LEAs in the two species by the same strategy used in this study.…”

Section: Resultsmentioning

confidence: 99%

“…thaliana [6, 8] and P . trichocarpa [17]. Comparing to five monocot species, two dicots have more LEA proteins in their genome.…”

Section: Resultsmentioning

confidence: 99%

“…To date, the genome-wide characterization of LEA family has been performed in several genome-sequenced plant species, such as Arabidopsis [6, 8], Populus trichocarpa [17], legumes [10], O . sativa [18], and Brachypodium distachyon [19].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis)

Huang

Zhong

et al. 2016

PLoS ONE

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Late embryogenesis abundant (LEA) proteins have been identified in a wide range of organisms and are believed to play a role in the adaptation of plants to stress conditions. In this study, we performed genome-wide identification of LEA proteins and their coding genes in Moso bamboo (Phyllostachys edulis) of Poaceae. A total of 23 genes encoding LEA proteins (PeLEAs) were found in P. edulis that could be classified to six groups based on Pfam protein family and homologous analysis. Further in silico analyses of the structures, gene amount, and biochemical characteristics were conducted and compared with those of O. sativa (OsLEAs), B. distachyon (BdLEAs), Z. mays (ZmLEAs), S. bicolor (SbLEAs), Arabidopsis, and Populus trichocarpa. The less number of PeLEAs was found. Evolutionary analysis revealed orthologous relationship and colinearity between P. edulis, O. sativa, B. distachyon, Z. mays, and S. bicolor. Analyses of the non-synonymous (Ka) and synonymous (Ks)substitution rates and their ratios indicated that the duplication of PeLEAs may have occurred around 18.8 million years ago (MYA), and divergence time of LEA family among the P. edulis-O. sativa and P. edulis–B. distachyon, P. edulis-S. bicolor, and P. edulis-Z. mays was approximately 30 MYA, 36 MYA, 48 MYA, and 53 MYA, respectively. Almost all PeLEAs contain ABA- and (or) stress-responsive regulatory elements. Further RNA-seq analysis revealed approximately 78% of PeLEAs could be up-regulated by dehydration and cold stresses. The present study makes insights into the LEA family in P. edulis and provides inventory of stress-responsive genes for further functional validation and transgenic research aiming to plant genetic improvement of abiotic stress tolerance.

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Section: Resultssupporting

confidence: 92%

“…Additionally, the LEA gene number of O . sativa varies in different studies [17, 18]. Therefore, we re-searched LEAs in the two species by the same strategy used in this study.…”

Section: Resultsmentioning

confidence: 99%

“…thaliana [6, 8] and P . trichocarpa [17]. Comparing to five monocot species, two dicots have more LEA proteins in their genome.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis)

Huang

Zhong

et al. 2016

PLoS ONE

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“…However, LEA family proteins have thus far only been investigated in a few plant species, such as Arabidopsis , rice, poplar, Chinese plum, legume, tomato, potato, barley and Pinus 910111213141516. In contrast, very few LEA genes have been identified in Orchidaceae, and studies on LEA family evolutionary relationships and functional characteristics are scarce.…”

mentioning

confidence: 99%

Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Ling

Guo

2016

Sci Rep

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Late embryogenesis abundant (LEA) proteins, a diverse family, accumulate during seed desiccation in the later stages of embryogenesis. LEA proteins are associated with tolerance to abiotic stresses, such as drought, salinity and high or cold temperature. Here, we report the first comprehensive survey of the LEA gene family in Dendrobium officinale, an important and widely grown medicinal orchid in China. Based on phylogenetic relationships with the complete set of Arabidopsis and Oryza LEA proteins, 17 genes encoding D. officinale LEAs (DofLEAs) were identified and their deduced proteins were classified into seven groups. The motif composition of these deduced proteins was correlated with the gene structure found in each LEA group. Our results reveal the DofLEA genes are widely distributed and expressed in tissues. Additionally, 11 genes from different groups were introduced into Escherichia coli to assess the functions of DofLEAs. Expression of 6 and 7 DofLEAs in E. coli improved growth performance compared with the control under salt and heat stress, respectively. Based on qPCR data, all of these genes were up-regulated in various tissues following exposure to salt and heat stresses. Our results suggest that DofLEAs play an important role in responses to abiotic stress.

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Genetically Modified Citrus: Current Status, Prospects, and Future Challenges

Tanwir

Soares

Welker

et al. 2020

Genetically Modified Crops

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Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa

Cited by 86 publications

References 33 publications

Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis)

Genome-Wide Identification, Characterization, and Stress-Responsive Expression Profiling of Genes Encoding LEA (Late Embryogenesis Abundant) Proteins in Moso Bamboo (Phyllostachys edulis)

Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Genetically Modified Citrus: Current Status, Prospects, and Future Challenges

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