Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Ling, Hong; Xu, Zhiai; Guo, Shun‐Xing

doi:10.1038/srep39693

Cited by 68 publications

(53 citation statements)

References 41 publications

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“…GmLEA2-1 was strongly expressed in root and pod, and relatively lower in stem and leaf ( Fig. 2a), which was consistent with the previous microarray and EST data [20]. Interestingly, GmLEA2-1 expression in early and late embryos was strikingly low, in accordance with the results, of the expression of At2g44060 [17].…”

Section: Expression Of Gmlea2-1 In Different Tissues Of Soybean and Usupporting

confidence: 91%

GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress

Wang

Shao

Zhang

et al. 2018

Acta Biologica Hungarica

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Late embryonic proteins (LEA) gene family was abundant mainly in higher plant embryos, which could protect the embryos from the damage caused by abiotic stress, especially drought and salt stresses. In the present study, GmLEA2-1 was cloned from soybean leaf tissue treated by 10% polyethylene glycol 6000 (PEG6000). The results of quantitative real-time PCR (qRT-PCR) revealed a variety of expression patterns of GmLEA2-1 in various tissues of soybean (root, stem, leaf, flower, pod, early embryo and late embryo). GmLEA2-1 gene shared a lower sequence similarity with other typical LEA genes of same group from different species, but similar functions. Overexpression of GmLEA2-1 in transgenic Arabidopsis thaliana conferred tolerance to drought and salt stresses. The fresh weight and dry weight of seedling, the primary root length and the lateral root density of transgenic Arabidopsis plants were higher than those of wild type Arabidopsis (WT) under drought and salt stresses. Cis-acting regulatory elements in the GmLEA2-1 promoter were also predicted. These data demonstrate that GmLEA2-1 protein play an important role in improving drought and salt tolerance in plants.

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Section: Expression Of Gmlea2-1 In Different Tissues Of Soybean and Usupporting

confidence: 91%

GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress

Wang

Shao

Zhang

et al. 2018

Acta Biologica Hungarica

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“…The protection to cells from dehydration is imparted by LEA (late embryogenesis-related proteins), osmotins, and dehydrins (Banerjee and Roychoudhury 2016). LEA proteins constitute a more widespread pool of proteins commonly termed 'hydrophillins' that defend other proteins from aggregation, desiccation, or osmotic stress (Ling et al 2016). Likewise, osmotins are highly regulated proteins that protect cells from high osmolarity and structural or metabolic disruptions (Le et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

et al. 2019

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Key message We describe here the recent developments about the involvement of diverse stress-related proteins in sensing, signaling, and defending the cells in plants in response to drought or/and heat stress. Abstract In the current era of global climate drift, plant growth and productivity are often limited by various environmental stresses, especially drought and heat. Adaptation to abiotic stress is a multigenic process involving maintenance of homeostasis for proper survival under adverse environment. It has been widely observed that a series of proteins respond to heat and drought conditions at both transcriptional and translational levels. The proteins are involved in various signaling events, act as key transcriptional activators and saviors of plants under extreme environments. A detailed insight about the functional aspects of diverse stress-responsive proteins may assist in unraveling various stress resilience mechanisms in plants. Furthermore, by identifying the metabolic proteins associated with drought and heat tolerance, tolerant varieties can be produced through transgenic/recombinant technologies. A large number of regulatory and functional stress-associated proteins are reported to participate in response to heat and drought stresses, such as protein kinases, phosphatases, transcription factors, and late embryogenesis abundant proteins, dehydrins, osmotins, and heat shock proteins, which may be similar or unique to stress treatments. Few studies have revealed that cellular response to combined drought and heat stresses is distinctive, compared to their individual treatments. In this review, we would mainly focus on the new developments about various stress sensors and receptors, transcription factors, chaperones, and stress-associated proteins involved in drought or/ and heat stresses, and their possible role in augmenting stress tolerance in crops.

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“…The seeds of Orchidaceae only have an embryo without an endosperm [23]. Seed-specific LEA genes in orchids have been poorly explored, although 17 LEA genes were identified from D. officinale and their role in the response to salt and heat stress has been characterized [24]. In this study, we performed a global-scale identification of LEA genes in the genomes of two orchids, P. equestris and D. officinale, classified and explored their expression pattern in different organs and developmental stages, and also characterized their participation in callus formation.…”

Section: Introductionmentioning

confidence: 99%

Identification and Classification of LEA Family Genes in Orchids and Characterization of Their Role in Callus Formation

Liu

Silva

et al. 2019

Preprint

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The plant late embryogenesis abundant (LEA) proteins are abundant in seeds, play an important role in various abiotic stresses. However, there is still no information on genome-wide identification of LEA genes in orchids and their function in callus formation is almost unknown. In this study, the LEA genes from two orchids (Phalaenopsis equestris and Dendrobium officinale), were genome-wide identified, classified and characterized. A total of 57 and 59 LEA genes were identified in the genomes and these were divided into 8 and 9 groups for P. equestris and D. officinale, respectively. The LEA_1 and LEA_4 genes from P. equestris and D. officinale showed strong expression in seeds, but were significantly down-regulated in flowers and absent in vegetative organs (leaves, stems and roots). In addition, the LEA_1 and LEA_4 genes from D. officinale were abundant in the protocorm-like body (PLB) stage, while weak signals that were detected in in vitro shoots could not be detected in plantlets. The expression of these genes highlights PLBs in orchids are somatic embryos. The DoLEA36 from LEA_4 and DoLEA43 from LEA_1 were further characterized. The GFP signal of the DoLEA36-GFP fusion protein was only detected in the cytoplasm, while the GFP signal of the DoLEA43-GFP fusion protein was detected in both the cytoplasm and nucleus. This indicates that DoEA36 localizes in the cytoplasm while DoLEA43 localizes in both the cytoplasm and nucleus. Both DoLEA36 and DoLEA43 stimulated callus formation in transgenic Arabidopsis. The percentage of callus formation from 35S::DoLEA43 transgenic lines was higher than in wild type plants in two callus induction methods. Our results provide comprehensive information about the LEA gene family in orchids and genetic evidence for the involvement of LEA genes in the induction of callus, which may reveal their positive role in the maintenance of PLBs in orchids.

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Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system

Cited by 68 publications

References 41 publications

GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress

GmLEA2-1, a late embryogenesis abundant protein gene isolated from soybean (Glycine max (L.) Merr.), confers tolerance to abiotic stress

Drought and heat stress-related proteins: an update about their functional relevance in imparting stress tolerance in agricultural crops

Identification and Classification of LEA Family Genes in Orchids and Characterization of Their Role in Callus Formation

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