Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato

Cai, Bingbing; Li, Qiang; Xu, Yongchao; Yang, Long; Bi, Huangai; Ai, Xizhen

doi:10.1016/j.plaphy.2016.07.019

Cited by 66 publications

(53 citation statements)

References 55 publications

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“…Different members of the FBA gene family have been identified and characterized in various plant species, including maize (Dennis et al, 1988), Arabidopsis (Lu et al, 2012), rice (Kagaya et al, 1995), spinach (Pelzer-Reith et al, 1993), tobacco (Yamada et al, 2000), Sesuvium portulacastrum (Fan et al, 2009), and tomato (Cai et al, 2016). FBA genes have been shown to be involved in various important physiological and biochemical processes, e.g., plant development (Zhang et al, 2014), signal transduction (Oelze et al, 2014), regulation of secondary metabolism (Zeng et al, 2014), plant defense and response to biotic (Mohapatra and Mittra, 2016), and abiotic stresses, including salt (Lu et al, 2012), cadmium (Sarry et al, 2006), drought (Khanna et al, 2014), chilling (Purev et al, 2008), and heat (Michelis and Gepstein, 2000), and post-translational modification (Mininno et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

Guo

Xie

et al. 2017

Front. Plant Sci.

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Fructose-1, 6-bisphosphate aldolase (FBA) is a key plant enzyme that is involved in glycolysis, gluconeogenesis, and the Calvin cycle. It plays significant roles in biotic and abiotic stress responses, as well as in regulating growth and development processes. In the present paper, 21 genes encoding TaFBA isoenzymes were identified, characterized, and categorized into three groups: class I chloroplast/plastid FBA (CpFBA), class I cytosol FBA (cFBA), and class II chloroplast/plastid FBA. By using a prediction online database and genomic PCR analysis of Chinese Spring nulli-tetrasomic lines, we have confirmed the chromosomal location of these genes in 12 chromosomes of four homologous groups. Sequence and genomic structure analysis revealed the high identity of the allelic TaFBA genes and the origin of different TaFBA genes. Numerous putative environment stimulus-responsive cis-elements have been identified in 1,500-bp regions of TaFBA gene promoters, of which the most abundant are the light-regulated elements (LREs). Phylogenetic reconstruction using the deduced protein sequence of 245 FBA genes indicated an independent evolutionary pathway for the class I and class II groups. Although, earlier studies have indicated that class II FBA only occurs in prokaryote and fungi, our results have demonstrated that a few class II CpFBAs exist in wheat and other closely related species. Class I TaFBA was predicted to be tetramers and class II to be dimers. Gene expression analysis based on microarray and transcriptome databases suggested the distinct role of TaFBAs in different tissues and developmental stages. The TaFBA 4–9 genes were highly expressed in leaves and might play important roles in wheat development. The differential expression patterns of the TaFBA genes in light/dark and a few abiotic stress conditions were also analyzed. The results suggested that LRE cis-elements of TaFBA gene promoters were not directly related to light responses. Most TaFBA genes had higher expression levels in the roots than in the shoots when under various stresses. Class I cytosol TaFBA genes, particularly TaFBA10/12/18 and TaFBA13/16, and three class II TaFBA genes are involved in responses to various abiotic stresses. Class I CpFBA genes in wheat are apparently sensitive to different stress conditions.

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Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

Guo

Xie

et al. 2017

Front. Plant Sci.

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“…Six of the 21 proteins including aldose reductase-related protein, phenylalanine ammonia-lyase (PAL), elicitor-responsive protein, aldo-keto reductase/oxidoreductase, and chloroplast fructose-bisphosphate aldolase (FBA), which play important roles in plant responses to biotic and abiotic stresses in wheat, were identified as under positive selection among microclimatic niches. Especially, enzyme activity of FBA detected in wheat was obviously influenced by temperature and light (Lv et al, 2011; Cai et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, enzyme activity of the wheat FBA (chloroplast fructose-bisphosphate aldolase) is obviously influenced by temperature and light, is related with plant response in all types of abiotic stresses (Lv et al, 2011; Cai et al, 2016), and appears to be under positive selection. This result may be explained by the known fact that the shady niche is under stresses of lower temperature and lower intensity of solar radiation in contrast to the sun niche during the growing season (October–May) of wild emmer wheat (Nevo et al, 1988; Li et al, 1999, 2002).…”

Section: Discussionmentioning

confidence: 99%

Solar Radiation-Associated Adaptive SNP Genetic Differentiation in Wild Emmer Wheat, Triticum dicoccoides

et al. 2017

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Whole-genome scans with large number of genetic markers provide the opportunity to investigate local adaptation in natural populations and identify candidate genes under positive selection. In the present study, adaptation genetic differentiation associated with solar radiation was investigated using 695 polymorphic SNP markers in wild emmer wheat originated in a micro-site at Yehudiyya, Israel. The test involved two solar radiation niches: (1) sun, in-between trees; and (2) shade, under tree canopy, separated apart by a distance of 2–4 m. Analysis of molecular variance showed a small (0.53%) but significant portion of overall variation between the sun and shade micro-niches, indicating a non-ignorable genetic differentiation between sun and shade habitats. Fifty SNP markers showed a medium (0.05 ≤ FST ≤ 0.15) or high genetic differentiation (FST > 0.15). A total of 21 outlier loci under positive selection were identified by using four different FST-outlier testing algorithms. The markers and genome locations under positive selection are consistent with the known patterns of selection. These results suggested that genetic differentiation between sun and shade habitats is substantial, radiation-associated, and therefore ecologically determined. Hence, the results of this study reflected effects of natural selection through solar radiation on EST-related SNP genetic diversity, resulting presumably in different adaptive complexes at a micro-scale divergence. The present work highlights the evolutionary theory and application significance of solar radiation-driven natural selection in wheat improvement.

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“…In our previous research, we found that low temperature (4°C) induced SlFBA7 expression, but the expression level decreased after 48 h of chilling stress. Overexpressing SlFBA7 enhanced the tolerance of seeds germination to a suboptimal temperature (18°C) (Cai et al ). It is known that the potential of plants to absorb light energy and funnel it to the reaction center is not altered under environmental stress.…”

Section: Discussionmentioning

confidence: 99%

“…We recently identified eight FBA genes ( SlFBAs ) from tomato plants ( Solanum lycopersicum ) and found that the mRNA levels of all SlFBAs and FBA activities were significantly altered in seedlings subjected to high or low temperatures (Cai et al ). This result indicates that the catalytic activity of FBA in the Calvin–Benson cycle is sensitive to temperature.…”

Section: Introductionmentioning

confidence: 99%

Decreasing fructose‐1,6‐bisphosphate aldolase activity reduces plant growth and tolerance to chilling stress in tomato seedlings

Cai

Liu

et al. 2018

Physiologia Plantarum

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In northern China, low temperature is the most common abiotic stresses for tomato plants cultivated in solar-greenhouse in winter. We recently found that the expression and enzyme activity of fructose-1,6-bisphosphate aldolases (FBAs) in tomato, which are important enzymes in the Calvin-Benson cycle (CBC), were significantly altered in tomato seedlings subjected to heat/cold stresses. In order to study the role of FBA in photosynthesis and in regulating cold stress responses of tomato seedlings (Solanum lycopersicum), we transformed a tomato inbred line (FF) with RNA interference (RNAi) vector containing SlFBA7 reverse tandem repeat sequence. We found that the decreased SlFBA7 expression led to the decreased activities of FBA, as well as the activities of other main enzymes in the CBC. We also noticed a decrease in net photosynthetic rate, ribulose-1,5-bisphosphate and soluble sugar content, stem diameter, dry weight and seed size in RNAi SlFBA7 plants compared to wild-type. However, there are no changes in starch contents in the RNAi transgenic plants. RNAi SlFBA7 plants showed a decreased germination rate, and an increased levels of superoxide anions (O ) and hydrogen peroxide (H O ) under low temperature (8/5°C) and low-light intensity (100 μmol m s photon flux density) growth conditions. These findings demonstrated the important role of SlFBA7 in regulating growth and chilling tolerance of tomato seedlings, and suggested that the catalytic activity of FBA in the CBC is sensitive to temperature.

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Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato

Cited by 66 publications

References 55 publications

Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

Solar Radiation-Associated Adaptive SNP Genetic Differentiation in Wild Emmer Wheat, Triticum dicoccoides

Decreasing fructose‐1,6‐bisphosphate aldolase activity reduces plant growth and tolerance to chilling stress in tomato seedlings

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