Genome-wide Analysis of Basic Helix-Loop-Helix Family Genes and Expression Analysis in Response to Drought and Salt Stresses in Hibiscus hamabo Sieb. et Zucc

Ni, Lei; Wang, Zhiquan; Fu, Zekai; Yu, Wanwen; Yin, Yongguang; Li, Huogen; Gu, Chao

doi:10.3390/ijms22168748

Cited by 18 publications

(12 citation statements)

References 39 publications

(43 reference statements)

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“…GWAS are used for investigating various genes and their multiple or complex traits in relation to different stresses [60]. The group II LEA genes in plant genomes exhibits diversity in size, sequence and complexity that is equally related to the diversity of their form and function in plants [61].…”

Section: Genome-wide Association Studies (Gwas) Of Group II Lea Proteinsmentioning

confidence: 99%

“…The group II LEA genes in plant genomes exhibits diversity in size, sequence and complexity that is equally related to the diversity of their form and function in plants [61]. GWAS of group II LEA proteins in different plants have identified novel group II LEA genes that were responsible for tolerance to biotic and abiotic stresses [60]. The outcomes of these investigations on different plant stresses will be essential for the selection of genes and designing of future crops.…”

Section: Genome-wide Association Studies (Gwas) Of Group II Lea Proteinsmentioning

confidence: 99%

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Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

et al. 2021

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In response to various environmental stresses, plants have evolved a wide range of defense mechanisms, resulting in the overexpression of a series of stress-responsive genes. Among them, there is certain set of genes that encode for intrinsically disordered proteins (IDPs) that repair and protect the plants from damage caused by environmental stresses. Group II LEA (late embryogenesis abundant) proteins compose the most abundant and characterized group of IDPs; they accumulate in the late stages of seed development and are expressed in response to dehydration, salinity, low temperature, or abscisic acid (ABA) treatment. The physiological and biochemical characterization of group II LEA proteins has been carried out in a number of investigations because of their vital roles in protecting the integrity of biomolecules by preventing the crystallization of cellular components prior to multiple stresses. This review describes the distribution, structural architecture, and genomic diversification of group II LEA proteins, with some recent investigations on their regulation and molecular expression under various abiotic stresses. Novel aspects of group II LEA proteins in Phoenix dactylifera and in orthodox seeds are also presented. Genome-wide association studies (GWAS) indicated a ubiquitous distribution and expression of group II LEA genes in different plant cells. In vitro experimental evidence from biochemical assays has suggested that group II LEA proteins perform heterogenous functions in response to extreme stresses. Various investigations have indicated the participation of group II LEA proteins in the plant stress tolerance mechanism, spotlighting the molecular aspects of group II LEA genes and their potential role in biotechnological strategies to increase plants’ survival in adverse environments.

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Section: Genome-wide Association Studies (Gwas) Of Group II Lea Proteinsmentioning

confidence: 99%

Section: Genome-wide Association Studies (Gwas) Of Group II Lea Proteinsmentioning

confidence: 99%

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

et al. 2021

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“…In addition, the transcription factor family has contributed to resisting adverse environmental factors in plants, such as drought resistance, salt tolerance, cold tolerance, plant iron deficiency stress, and so on (Toledo-Ortiz et al, 2003). A great many bHLH genes have been distinguished in the plant kingdom, including A. thaliana (162) (Pires and Dolan, 2010), Hibiscus hamabo (162) (Ni et al, 2021), Helianthus annuus (183) (Li et al, 2021), Capsicum annuum (107) , Sorghum bicolor (174) (Fan et al, 2021), Osmanthus fragrans (206) (Li et al, 2020), Camellia sinensis (134) , Juglans regia (102) (Zhao et al, 2021), Pyrus bretschneideri (197) (Dong et al, 2021). The number of bHLH transcription factors changed widely among various plants.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in Prunus mume

Ding

et al. 2021

Front. Genet.

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Prunus mume is an illustrious ornamental woody plant with colorful flowers, delicate fragrances, and graceful tree forms. Low temperature limits its geographical distribution. The basic helix-loop-helix (bHLH) proteins exist in most eukaryotes as a transcription factor superfamily, which play a crucial role in metabolism, physiology, development, and response to various stresses of higher organisms. However, the characteristics of the bHLH gene family and low-temperature response remain unknown in P. mume. In the present study, we distinguished 95 PmbHLH genes in the P. mume whole-genome and analyzed their features. PmbHLHs were divided into 23 subfamilies and one orphan by phylogenetic analysis. Similar gene structures and conserved motifs appeared in the same subfamily. These genes were situated in eight chromosomes and scaffolds. Gene duplication events performed a close relationship to P. mume, P. persica, and P. avium. Tandem duplications probably promoted the expansion of PmbHLHs. According to predicted binding activities, the PmbHLHs were defined as the Non-DNA-binding proteins and DNA-binding proteins. Furthermore, PmbHLHs exhibited tissue-specific and low-temperature induced expression patterns. By analyzing transcriptome data, 10 PmbHLHs which are responsive to low-temperature stress were selected. The qRT-PCR results showed that the ten PmbHLH genes could respond to low-temperature stress at different degrees. There were differences in multiple variations among different varieties. This study provides a basis to research the evolution and low-temperature tolerance of PmbHLHs, and might enhance breeding programs of P. mume by improving low-temperature tolerance.

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“…Quantitative real-time PCR was determined using the mature method [ 35 ]. The PCR instrument and reagent were a StepOnePlus real-time PCR system (Applied Biosystems) and a SYBR Green Master Mix (Bimake, Houston, TX, USA), respectively.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Characterization of NAC Family in Hibiscus hamabo Sieb. et Zucc. under Various Abiotic Stresses

Wang

et al. 2022

IJMS

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NAC transcription factor is one of the largest plant gene families, participating in the regulation of plant biological and abiotic stresses. In this study, 182 NAC proteins (HhNACs) were identified based on genomic datasets of Hibiscus hamabo Sieb. et Zucc (H. hamabo). These proteins were divided into 19 subfamilies based on their phylogenetic relationship, motif pattern, and gene structure analysis. Expression analysis with RNA-seq revealed that most HhNACs were expressed in response to drought and salt stress. Research of quantitative real-time PCR analysis of nine selected HhNACs supported the transcriptome data’s dependability and suggested that HhNAC54 was significantly upregulated under multiple abiotic stresses. Overexpression of HhNAC54 in Arabidopsis thaliana (A. thaliana) significantly increased its tolerance to salt. This study provides a basis for a comprehensive analysis of NAC transcription factor and insight into the abiotic stress response mechanism in H. hamabo.

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Genome-wide Analysis of Basic Helix-Loop-Helix Family Genes and Expression Analysis in Response to Drought and Salt Stresses in Hibiscus hamabo Sieb. et Zucc

Cited by 18 publications

References 39 publications

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

Plant Group II LEA Proteins: Intrinsically Disordered Structure for Multiple Functions in Response to Environmental Stresses

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in Prunus mume

Genome-Wide Identification and Characterization of NAC Family in Hibiscus hamabo Sieb. et Zucc. under Various Abiotic Stresses

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