Genome-Wide Identification and Characterization of NAC Family in Hibiscus hamabo Sieb. et Zucc. under Various Abiotic Stresses

Wang, Zhiquan; Ni, Lei; Yu, Wanwen; Fu, Zekai; Hua, Jianfeng; Lü, Zhiguo; Liu, Liangqin; Yin, Yongguang; Li, Huogen; Gu, Chao

doi:10.3390/ijms23063055

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Plant growth and development, such as seed germination, secondary wall formation and lateral root development, are regulated by NAC transcription factors [ 79 ]. NAC transcription factors are modulated by environmental stimuli, phytohormones and miRNAs and play important regulatory roles in plants subjected to abiotic stresses such as salinity, drought and cold [ 80 ]. Under salt stress, NAC13-overexpressing transgenic poplar plants exhibited enhanced salt tolerance, whereas NAC13-suppressed plants were more sensitive to salt stress [ 81 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Wang,

Jiang,

et al. 2024

BMC Plant Biol

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Seed propagation is the main method of mulberry expansion in China, an important economic forest species. However, seed germination is the most sensitive stage to various abiotic stresses, especially salinity stress. To reveal the molecular regulatory mechanism of mulberry seed germination under salt stress, flavonoid metabolomics and transcriptomics analyses were performed on mulberry seeds germinated under 50 and 100 mmol/L NaCl stress. Analysis of the flavonoid metabolome revealed that a total of 145 differential flavonoid metabolites (DFMs) were classified into 9 groups, 40 flavonols, 32 flavones, 16 chalcones and 14 flavanones. Among them, 61.4% (89) of the DFMs accumulated continuously with increasing salt concentration, reaching the highest level at a 100 mmol/L salt concentration; these DFMs included quercetin-3-O-glucoside (isoquercitrin), kaempferol (3,5,7,4'-tetrahydroxyflavone), quercetin-7-O-glucoside, taxifolin (dihydroquercetin) and apigenin (4',5,7-trihydroxyflavone), indicating that these flavonoids may be key metabolites involved in the response to salt stress. Transcriptional analysis identified a total of 3055 differentially expressed genes (DEGs), most of which were enriched in flavonoid biosynthesis (ko00941), phenylpropanoid biosynthesis (ko00940) and biosynthesis of secondary metabolites (ko01110). Combined analysis of flavonoid metabolomic and transcriptomic data indicated that phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), flavonol synthase (FLS), bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase (DFR) and anthocyanidin reductase (ANR) were the key genes involved in flavonoid accumulation during mulberry seed germination under 50 and 100 mmol/L NaCl stress. In addition, three transcription factors, MYB, bHLH and NAC, were involved in the regulation of flavonoid accumulation under salt stress. The results of quantitative real-time PCR (qRT‒PCR) validation showed that the expression levels of 11 DEGs, including 7 genes involved in flavonoid biosynthesis, under different salt concentrations were consistent with the transcriptomic data, and parallel reaction monitoring (PRM) results showed that the expression levels of 6 key enzymes (proteins) involved in flavonoid synthesis were consistent with the accumulation of flavonoids. This study provides a new perspective for investigating the regulatory role of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress at different concentrations.

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Section: Discussionmentioning

confidence: 99%

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Wang,

Jiang,

et al. 2024

BMC Plant Biol

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“…et Zucc.) in Arabidopsis enhanced salt tolerance, and SlNAC10 (from Suaeda liaotungensis) in Arabidopsis in addition to salt enhanced drought tolerance [1,19,20]. Additionally, the NAC transcription factor ATAF1 (Arabidopsis Transcription Activation Factor 1) was found to respond to carbon starvation by participating in trehalose metabolism.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Investigation of the NAC Transcription Factor Family in Apocynum venetum Revealed Their Synergistic Roles in Abiotic Stress Response and Trehalose Metabolism

Huang

Qiu

Wang

et al. 2023

IJMS

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NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) are one of the most prominent plant-specific TF families and play essential roles in plant growth, development and adaptation to abiotic stress. Although the NAC gene family has been extensively characterized in many species, systematic analysis is still relatively lacking in Apocynum venetum (A. venetum). In this study, 74 AvNAC proteins were identified from the A. venetum genome and were classified into 16 subgroups. This classification was consistently supported by their gene structures, conserved motifs and subcellular localizations. Nucleotide substitution analysis (Ka/Ks) showed the AvNACs to be under the influence of strong purifying selection, and segmental duplication events were found to play the dominant roles in the AvNAC TF family expansion. Cis-elements analysis demonstrated that the light-, stress-, and phytohormone-responsive elements being dominant in the AvNAC promoters, and potential TFs including Dof, BBR-BPC, ERF and MIKC_MADS were visualized in the TF regulatory network. Among these AvNACs, AvNAC58 and AvNAC69 exhibited significant differential expression in response to drought and salt stresses. The protein interaction prediction further confirmed their potential roles in the trehalose metabolism pathway with respect to drought and salt resistance. This study provides a reference for further understanding the functional characteristics of NAC genes in the stress-response mechanism and development of A. venetum.

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“…Some studies have identified and analyzed members of the NAC gene family in plant genomes such as Arabidopsis thaliana ( Hisako et al., 2004 ), Zanthoxylum bungeanum ( Hu et al., 2022 ), potato ( Singh et al., 2013 ), melon ( Wei et al., 2016 ), sunflower ( Bengoa Luoni et al., 2021 ), Asparagus officinalis ( Li C. et al., 2022 ), Brassica juncea var. Tumida ( He et al., 2020 ), Saccharum spontaneum ( Shen et al., 2022 ) and Hibiscus hamabo Sieb ( Wang et al., 2022b ), but there is no relevant research report on the NAC gene family in D. nobile ’s whole genome. In this study, bioinformatics methods were used to conduct a comprehensive analysis of the physicochemical properties, chromosomal positioning, and conserved motifs of the D. nobile NAC gene family (named: DnoNAC ), and the resulting DnoNAC proteins were compared in multiple sequences to construct an phylogenetic tree to analyze their molecular evolutionary relationships, with the goal of laying the groundwork for future research on the functions of the D. nobile NAC .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and molecular evolution of NAC gene family in Dendrobium nobile

Fu,

Liu

2023

Front. Plant Sci.

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NAC transcription factors are an important genes that regulate plant growth and development, and can regulate functions such as fruit ripening in plants. Based on genome data of Dendrobium nobile, the NAC gene family was identified and analyzed by bioinformatics methods. In this study, we identified 85 NAC genes in Dendrobium nobile genome, and systematically analyzed the NAC gene family. We found that they were distributed unevenly in the nineteen chromosomes. The amino acid length of D. nobile NAC gene family (DnoNACs) ranged from 80 to 1065, molecular weight ranged from 22.17 to 119.02 kD, and isoelectric point ranged from 4.61~9.26. Its promoter region contains multiple stress responsive elements, including light responsive, gibberellin-responsive, abscisic acid responsiveness, MeJA-responsiveness and drought-inducibility elements. Phylogenetic analysis indicates that the D. nobile NAC gene family is most closely related to Dendrobium catenatum and Dendrobium chrysotoxum. Analysis of SSR loci indicates that the fraction of mononucleotide repeats was the largest, as was the frequency of A/T. Non-coding RNA analysis showed that these 85 NAC genes contain 397 miRNAs. The collinearity analysis shows that 9 collinear locis were found on the chromosomes of D. nobile with Arabidopsis thaliana, and 75 collinear locis with D.chrysotoxum. QRT-PCR experiment under different salt concentration and temperature conditions verified the response mechanism of DnoNAC gene family under stress conditions. Most DnoNAC genes are sensitive to salt stress and temperature stress. The results of this study provide a reference for further understanding the function of NAC gene in D. nobile.

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Genome-Wide Identification and Characterization of NAC Family in Hibiscus hamabo Sieb. et Zucc. under Various Abiotic Stresses

Cited by 11 publications

References 33 publications

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Genome-Wide Investigation of the NAC Transcription Factor Family in Apocynum venetum Revealed Their Synergistic Roles in Abiotic Stress Response and Trehalose Metabolism

Genome-wide identification and molecular evolution of NAC gene family in Dendrobium nobile

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