Genome-Wide Analyses of Amphioxus MicroRNAs Reveal an Immune Regulation via miR-92d Targeting C3

Yang, Rongliang; Zheng, Tingting; Cai, Xingsheng; Yu, Yaoguang; Yu, Cuiling; Guo, Lei; Huang, Shengfeng; Zhu, Wei; Zhu, Ruimin; Yan, Qingyu; Ren, Zhenghua; Chen, Shangwu; Xu, Anlong

doi:10.4049/jimmunol.1200801

Cited by 27 publications

(21 citation statements)

References 52 publications

(71 reference statements)

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“…The complement system plays a key role in lupus pathogenesis, but their regulation and mechanism of action remain incompletely understood. Studies that determine the association of complement proteins and miRNA are limited . This study attempts to determine the connecting links between the complement protein, C5a and miRNA.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional profiling reveals that C5a alters microRNA in brain endothelial cells

et al. 2014

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SummaryBlood-brain barrier (BBB) disturbance is a crucial occurrence in many neurological diseases, including systemic lupus erythematosus (SLE). Our previous studies showed that experimental lupus serum altered the integrity of the mouse brain endothelial layer, an important constituent of the BBB. Complement activation occurs in lupus with increased circulating complement components. Using a genomics approach, we identified the microRNA (miRNA) altered in mouse brain endothelial cells (bEnd3) by lupus serum and the complement protein, C5a. Of the 318 miRNA evaluated, 23 miRNAs were altered by lupus serum and 32 were altered by C5a alone compared with controls. Seven miRNAs (P < 0Á05) were differentially expressed by both treatments: mmu-miR-133a*, mmu-miR-193*, mmu-miR-26b, mmu-miR-28*, mmu-miR-320a, mmu-miR-423-3p and mmu-miR-509-5p. The microarray results were validated by quantitative RT-PCR. In line with the in vitro results, expression of miR-26b and miR-28* were also significantly up-regulated in lupus mouse brain which was reduced by C5a receptor inhibition. Target prediction analysis revealed miR gene targets encoding components involved in inflammation, matrix arrangement, and apoptosis, pathways known to play important roles in central nervous system lupus. Our findings suggest that the miRNAs reported in this study may represent novel therapeutic targets in central nervous system lupus and other similar neuroinflammatory settings.

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Section: Discussionmentioning

confidence: 99%

“…Studies that determine the association of complement proteins and miRNA are limited. 15,53,54 This study attempts to determine the connecting links between the complement protein, C5a and miRNA. To our knowledge, this is the first study demonstrating the miRNA expression profile in brain endothelial cells and its regulation by complement.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling reveals that C5a alters microRNA in brain endothelial cells

et al. 2014

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“…The genome of one of the Asian species ( B. belcheri ) is available on a genome browser (http://mosas.sysu.edu.cn/genome/gbrowser_wel.php, last accessed September 27, 2014) (Huang et al 2012), while sequencing of the genome of the European species ( B. lanceolatum ) is in progress (http://www.cns.fr/spip/-Branchiostoma-lanceolatum,585-.html, last accessed September 27, 2014). Given the conservation between the B. floridae and vertebrate genomes as well as the rather subtle morphological differences among the various species of Brachiostoma , it is perhaps not surprising that such genomic features as microRNAs (miRNAs) are highly conserved between Branchiostoma species (Dai et al 2009; Yang et al 2013). …”

Section: Introductionmentioning

confidence: 99%

The Transcriptome of an Amphioxus, Asymmetron lucayanum, from the Bahamas: A Window into Chordate Evolution

Putnam

Holland

2014

Genome Biology and Evolution

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Cephalochordates, the sister group of tunicates plus vertebrates, have been called “living fossils” due to their resemblance to fossil chordates from Cambrian strata. The genome of the cephalochordate Branchiostoma floridae shares remarkable synteny with vertebrates and is free from whole-genome duplication. We performed RNA sequencing from larvae and adults of Asymmetron lucayanum, a cephalochordate distantly related to B. floridae. Comparisons of about 430 orthologous gene groups among both cephalochordates and 10 vertebrates using an echinoderm, a hemichordate, and a mollusk as outgroups showed that cephalochordates are evolving more slowly than the slowest evolving vertebrate known (the elephant shark), with A. lucayanum evolving even more slowly than B. floridae. Against this background of slow evolution, some genes, notably several involved in innate immunity, stand out as evolving relatively quickly. This may be due to the lack of an adaptive immune system and the relatively high levels of bacteria in the inshore waters cephalochordates inhabit. Molecular dating analysis including several time constraints revealed a divergence time of ∼120 Ma for A. lucayanum and B. floridae. The divisions between cephalochordates and vertebrates, and that between chordates and the hemichordate plus echinoderm clade likely occurred before the Cambrian.

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“…miRNA is a type of untranslated RNA, and 50–75% of them control transcription and translation with help of binding target mRNA (5). MRSA expresses various types of miRNA abnormally, in particular, the markedly upregulated miR-7 (6). …”

Section: Introductionmentioning

confidence: 99%

Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus

Zhang

Liu

et al. 2016

Experimental and Therapeutic Medicine

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The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus (MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmid in vitro. Following transfection of the bacterial strain, the effect of β-lactam antibiotics in minimum inhibitory concentration (MIC) was observed using the microporous dilution method, and antibacterial effects in vitro were observed using the dynamic growth curve method. The expression of miR-7 in sensitive MRSA was upregulated distinctly, with significant difference (P<0.05). MIC and the number of bacteria in the miR-7 overexpression group significantly increased while the inhibition group decreased prominently, with significant difference (P<0.05). The control and null plasmid groups revealed no significant difference. In conclusion, miR-7 upregulated the antimicrobial activity of MRSA, and the intervention of its expression may become a possible antibacterial target.

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Genome-Wide Analyses of Amphioxus MicroRNAs Reveal an Immune Regulation via miR-92d Targeting C3

Cited by 27 publications

References 52 publications

Transcriptional profiling reveals that C5a alters microRNA in brain endothelial cells

Transcriptional profiling reveals that C5a alters microRNA in brain endothelial cells

The Transcriptome of an Amphioxus, Asymmetron lucayanum, from the Bahamas: A Window into Chordate Evolution

Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus

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