Genome Variability of Russian Potato Cultivars: Aflp-Analysis Data

Dyachenko, E. A.

doi:10.15389/agrobiology.2020.3.499eng

Cited by 2 publications

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“…In addition, AFLP is popular in modern plant breeding and is used to determine pedigrees, variability, homogeneity, and the degree of introgression and hybridity of varieties, as well as to search for molecular markers associated with economically valuable traits (Mba, Tohme, 2005;Swiecicka et al, 2009;Arif et al, 2010). Such studies have been carried out, for example, on wheat (Hassan et al, 2018), barley (El-Esawi et al, 2018a), peas (D'iachenko et al, 2014;El-Esawi et al, 2018b), pepper (Kochieva, Ryzhova, 2009) and potato (McGregor et al, 2002;Jacobs et al, 2008;Bamberg, del Rio, 2014;Bryan et al, 2017;Dyachenko et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genome variability of domestic tomato varieties: data from AFLP analysis

et al. 2022

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Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim of this work was to evaluate the genome variability of 59 cultivars and perspective breeding lines of S. lycopersicum and 11 wild tomato species using the AFLP method. According to the AFLP analysis, four combinations of primers E32/M59, E32/M57, E38/M57, and E41/M59, which had the highest PIC (polymorphism information content) values, were selected. In the process of genotyping a collection of 59 cultivars/lines of S. lycopersicum and 11 wild tomato accessions, the selected primers revealed 391 fragments ranging in size from 80 to 450 bp, of which 114 fragments turned out to be polymorphic and 25 were unique. Analysis of the amplif ication spectra placed wild tomato accessions into separate clades. Sister clades included cultivars of FSCV breeding resistant to drought and/or cold and, in part, to late blight, Alternaria, Septoria, tobacco mosaic virus and blossom end rot, as well as tomato accessions not characterized according to these traits, which suggests that they have resistance to stress factors. In accessions of distant clades, there was clustering on the basis of resistance to Verticillium, cladosporiosis, Fusarium, tobacco mosaic virus, gray rot, and blossom end rot. The combination of accessions according to their origin from the originating organization was shown. The primer combinations E32/M59, E32/M57, E38/M57 and E41/M59 were shown to be perspective for genotyping tomato cultivars to select donors of resistance to various stress factors. The clade-specif ic fragments identif ied in this work can become the basis for the development of AFLP markers for traits of resistance to stress factors.

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Section: Introductionmentioning

confidence: 99%

Genome variability of domestic tomato varieties: data from AFLP analysis

et al. 2022

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Nomenclatural standards and genetic passports of potato cultivars bred in the A.G. Lorkh All-Russian Research Institute of Potato Farming

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Antonova

Chukhina

et al. 2021

Biotehnologiâ i selekciâ rastenij

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In order to preserve the genetic diversity of cultivars, it is recommended to develop documentation approaches through the registration of cultivar nomenclatural standards in accordance with the International Code of Nomenclature for Cultivated Plants. The N.I Vavilov Institute of Plant Genetic Resources keeps developing this approach along with the DNA marker technologies: SSR genotyping and molecular screening with DNA markers of genes controlling valuable traits. In 2018, VIR, together with the leading national centre for potato breeding – the A.G. Lorkh All-Russian Scientific-Research Institute of Potato Farming (now the A.G. Lorkh Russian Potato Research Center), initiated the preparation of nomenclatural standards for potato cultivars bred both at the A.G. Lorkh institute and in co-authorship with other breeding institutions. This paper presents 30 nomenclatural standards which are maintained in the Nomenclature standard fund of the Herbarium of cultivated plants and their wild relatives and weeds (VIR Herbarium, WIR). Also, the paper presents genetic passports of the mentioned 30 cultivars, developed using DNA samples extracted from the plant material of nomenclatural standards. Genetic passports include information about the polymorphism of the 8 chromosome-specific microsatellites and data on the results of molecular screening with 15 markers of 11 genes conferring resistance to the most dangerous pathogens and pests: potato viruses Y and X, late blight and potato cyst nematodes. A comparison of SSR-profiles of nomenclatural standards with those of 66 cultivar samples obtained from various sources made it possible to verify authenticity and homogeneity of the latter.

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Genome Variability of Russian Potato Cultivars: Aflp-Analysis Data

Cited by 2 publications

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Genome variability of domestic tomato varieties: data from AFLP analysis

Genome variability of domestic tomato varieties: data from AFLP analysis

Nomenclatural standards and genetic passports of potato cultivars bred in the A.G. Lorkh All-Russian Research Institute of Potato Farming

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